High-yield production and characterization of biologically active GST-tagged human topoisomerase IIα protein in insect cells for the development of a high-throughput assay

2011 ◽  
Vol 76 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Praveen K. Singh ◽  
Pan F. Chan ◽  
Martin J. Hibbs ◽  
Maria-Jesus Vazquez ◽  
Delfina C. Segura ◽  
...  
Keyword(s):  
High Throughput ◽  
Insect Cells ◽  
Biologically Active ◽  
High Yield ◽  
Topoisomerase Iiα ◽  
Yield Production ◽  
High Throughput Assay ◽  
High Yield Production

High-yield production and characterization of biologically active recombinant aprotinin expressed in Saccharomyces cerevisiae

2009 ◽  
Vol 66 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Akihiro Meta ◽  
Hiroshi Nakatake ◽  
Takayuki Imamura ◽  
Chikateru Nozaki ◽  
Kazuhisa Sugimura
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biologically Active ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

scholarly journals Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes

2020 ◽  
Author(s):  
Ching-Lin Hsieh ◽  
Jory A. Goldsmith ◽  
Jeffrey M. Schaub ◽  
Andrea M. DiVenere ◽  
Hung-Che Kuo ◽  
...  
Keyword(s):  
Room Temperature ◽  
Protein A ◽  
Public Health Emergency ◽  
High Yield ◽  
The Novel ◽  
Yield Production ◽  
Novel Coronavirus ◽  
Serological Diagnostics ◽  
High Yield Production

ABSTRACTThe COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has led to accelerated efforts to develop therapeutics, diagnostics, and vaccines to mitigate this public health emergency. A key target of these efforts is the spike (S) protein, a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large quantities. Here, we designed and expressed over 100 structure-guided spike variants based upon a previously determined cryo-EM structure of the prefusion SARS-CoV-2 spike. Biochemical, biophysical and structural characterization of these variants identified numerous individual substitutions that increased protein yields and stability. The best variant, HexaPro, has six beneficial proline substitutions leading to ∼10-fold higher expression than its parental construct and is able to withstand heat stress, storage at room temperature, and multiple freeze-thaws. A 3.2 Å-resolution cryo-EM structure of HexaPro confirmed that it retains the prefusion spike conformation. High-yield production of a stabilized prefusion spike protein will accelerate the development of vaccines and serological diagnostics for SARS-CoV-2.

scholarly journals Review: Managing sheep and goats for sustainable high yield production

animal ◽  
2021 ◽  
pp. 100293
Author(s):  
J. Simões ◽  
J.A. Abecia ◽  
A. Cannas ◽  
J.A. Delgadillo ◽  
D. Lacasta ◽  
...  
Keyword(s):  
High Yield ◽  
Sheep And Goats ◽  
Yield Production ◽  
High Yield Production
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