FhuA deletion variant Δ1-159 overexpression in inclusion bodies and refolding with Polyethylene-Poly(ethylene glycol) diblock copolymer

2011 ◽  
Vol 77 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Tamara Dworeck ◽  
Anne-Kathrin Petri ◽  
Noor Muhammad ◽  
Marco Fioroni ◽  
Ulrich Schwaneberg
Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 195 ◽  
Author(s):  
Abdullah M Alswieleh ◽  
Abeer M Beagan ◽  
Bayan M Alsheheri ◽  
Khalid M Alotaibi ◽  
Mansour D Alharthi ◽  
...  

This paper introduces the synthesis of well-defined 2-(tert-butylamino)ethyl methacrylate-b-poly(ethylene glycol) methyl ether methacrylate diblock copolymer, which has been grafted onto mesoporous silica nanoparticles (PTBAEMA-b-PEGMEMA-MSNs) via atom transfer radical polymerization (ATRP). The ATRP initiators were first attached to the MSN surfaces, followed by the ATRP of 2-(tert-butylamino)ethyl methacrylate (PTBAEMA). CuBr2/bipy and ascorbic acid were employed as the catalyst and reducing agent, respectively, to grow a second polymer, poly(ethylene glycol) methyl ether methacrylate (PEGMEMA). The surface structures of these fabricated nanomaterials were then analyzed using Fourier Transform Infrared (FTIR) spectroscopy. The results of Thermogravimetric Analysis (TGA) show that ATRP could provide a high surface grafting density for polymers. Dynamic Light Scattering (DLS) was conducted to investigate the pH-responsive behavior of the diblock copolymer chains on the nanoparticle surface. In addition, multifunctional pH-sensitive PTBAEMA-b-PEGMEMA-MSNs were loaded with doxycycline (Doxy) to study their capacities and long-circulation time.


2016 ◽  
Vol 4 (5) ◽  
pp. 857-862 ◽  
Author(s):  
Liangliang Shen ◽  
Rui Qu ◽  
Hejin Shi ◽  
Fan Huang ◽  
Yingli An ◽  
...  

Herein, a complex micelle as an oxygen nano-carrier is constructed through the hierarchical assembly of the diblock copolymer poly(ethylene glycol)-block-poly(l-lysine) (PEG-b-PLys), tetrakis(4-sulfonatophenyl)porphinato cobalt(ii) (Co(ii)TPPS), a heptapeptide (Cys-His-His-His-His-His-His) and heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin (TM-β-CD).


2009 ◽  
Vol 009 (12) ◽  
pp. 1232-1237 ◽  
Author(s):  
Guolin WU ◽  
Zheng WANG ◽  
Hai LIN ◽  
Jie ZHANG ◽  
Pingchuan SUN ◽  
...  

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