High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system

2021 ◽  
pp. 106025
Author(s):  
Muhammad Ashir Zia ◽  
Muhammad Salahuddin Shah ◽  
Rai Shafqat Ali Khan ◽  
Umer Farooq ◽  
Jamila Shafi ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Fusion System ◽  
Structural Protein ◽  
Expression And Purification ◽  
High Level Expression ◽  
Mouth Disease Virus ◽  
Sumo Fusion ◽  
High Level

scholarly journals Foot-and-mouth disease virus non-structural protein 3A modulates cellular innate immune responses to influence host tropism

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Soumendu Chakravarti ◽  
Caroline Wright ◽  
Emma Howes ◽  
Richard Kock ◽  
Terry Jackson ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Economic Losses ◽  
Structural Protein ◽  
Host Tropism ◽  
C Terminus ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Species Specific

The picornavirus foot-and-mouth disease virus (FMDV) is responsible for one of the most significant diseases of livestock, leading to large economic losses due to reduced productivity and trade embargoes for areas not certified as disease-free. The picornavirus non-structural protein 3A is involved in replication of the viral RNA genome and is implicated in host tropism of several picornaviruses. Deletions in the C-terminus of 3A have been observed in FMDV outbreaks specific for swine and such viruses are non-pathogenic in cattle. The mechanism for species specific attenuation of FMDV is unknown. We have shown that FMDV containing a C-terminal deletion in 3A is attenuated in bovine cell culture and that the attenuated phenotype can be reversed by the JAK1/2 inhibitor Ruxolitinib (Rux), identifying a role for the induction of interferon stimulated genes (ISGs) in the restricted bovine tropism of the 3A-deleted virus.

scholarly journals Early origin and global colonisation of foot-and-mouth disease virus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Pakorn Aiewsakun ◽  
Nakarin Pamornchainavakul ◽  
Chaidate Inchaisri
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Epidemiological Data ◽  
Mouth Disease ◽  
Recent Common Ancestor ◽  
Structural Protein ◽  
Most Recent Common Ancestor ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Early Origin

Abstract In this study, we compiled 84-year worth (1934–2017) of genomic and epidemiological data of foot-and-mouth disease virus (FMDV), and performed comprehensive analyses to determine its early origin and transmission route. We found that recombination is a key feature of FMDV, and that the genomic regions coding for structural and non-structural proteins have markedly different evolutionary histories, and evolve at different rates. Despite all of these differences, analyses of both structural and non-structural protein coding regions consistently suggested that the most recent common ancestor of FMDV could be dated back to the Middle Age, ~ 200 to 300 years earlier than previously thought. The ancestors of the Euro-Asiatic and SAT strains could be dated back to the mid-seventeenth century, and to the mid-fifteenth to mid-sixteenth century, respectively. Our results implicated Mediterranean counties as an early geographical origin of FMDV before spreading to Europe and subsequently to Asia and South America.

scholarly journals Generation of monoclonal antibodies against non-structural protein 3AB of foot-and-mouth disease virus

2012 ◽  
Vol 27 (5) ◽  
pp. 316-319 ◽  
Author(s):  
Tong Lin ◽  
Junjun Shao ◽  
Huiyun Chang ◽  
Shandian Gao ◽  
Guozheng Cong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth

High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts

2005 ◽  
Vol 25 (4) ◽  
pp. 329-333 ◽  
Author(s):  
Yinü Li ◽  
Meng Sun ◽  
Jixing Liu ◽  
Zongqi Yang ◽  
Zhifang Zhang ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
High Expression ◽  
Structural Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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