Expression responses of nine cytochrome P450 genes to xenobiotics in the cotton bollworm Helicoverpa armigera

2010 ◽  
Vol 97 (3) ◽  
pp. 209-213 ◽  
Author(s):  
Xiaojie Zhou ◽  
Chengfa Sheng ◽  
Mei Li ◽  
Hua Wan ◽  
Dong Liu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Helicoverpa Armigera ◽  
Cotton Bollworm ◽  
Cytochrome P450 Genes ◽  
Helicoverpa Armígera

Effects of dietary quercetin on performance and cytochrome P450 expression of the cotton bollworm, Helicoverpa armigera

2015 ◽  
Vol 105 (6) ◽  
pp. 771-777 ◽  
Author(s):  
D. Liu ◽  
Y. Yuan ◽  
M. Li ◽  
X. Qiu
Keyword(s):  
Cytochrome P450 ◽  
Helicoverpa Armigera ◽  
Adult Emergence ◽  
Cotton Bollworm ◽  
Molecular Response ◽  
Terrestrial Plants ◽  
P450 Expression ◽  
Helicoverpa Armígera

AbstractQuercetin is ubiquitous in terrestrial plants. The cotton bollworm Helicoverpa armigera as a highly polyphagous insect has caused severe crop losses. Until now, interactions between this pest and quercetin are poorly understood at the biochemical and molecular levels. In this study, we investigated the in vivo effects of quercetin on performance of cotton bollworm and on cytochrome P450 (P450) expression. Deleterious effects of quercetin on the performance of the cotton bollworm, including growth, survival, pupation and adult emergence were observed after oral administration of 3 and 10 mg g−1 quercetin to larvae since the third instar, whereas no significant toxic effect was found at 0.1 mg g−1 quercetin treatment. Piperonyl butoxide treatment enhanced the toxicity of quercetin. In vitro metabolism studies showed that quercetin was rapidly transformed by gut enzymes of fifth instar larvae of the cotton bollworm. qRT–PCR results revealed that the effect of quercetin on P450 expression was tissue- and dose-specific. Quercetin regulated P450 expression in a mild manner, and it could serve as P450 inducer (CYP337B1, CYP6B6) or repressor (CYP337B1, CYP6B7, CYP6B27, CYP9A14, CYP6AE11, and CYP4M7). These findings are important for advancing our understanding of the biochemical and molecular response of insects to plant toxins and have implications for a smart pest control.

scholarly journals Overexpression of Cytochrome P450 CYP6B7 Mediated Pyrethroid Resistance in Indian Strains of the Cotton Bollworm, Helicoverpa Armigera (Hübner)

2014 ◽  
Vol 54 (3) ◽  
pp. 287-293 ◽  
Author(s):  
Ashraf Oukasha Abd El-Latif ◽  
Keshav Raj Kranthi ◽  
Sandhya Kranthi ◽  
Ali Sarwar ◽  
Bhattiprolu Subrahmanyam
Keyword(s):  
Cytochrome P450 ◽  
Helicoverpa Armigera ◽  
Pyrethroid Resistance ◽  
Cytochrome P450 Monooxygenase ◽  
Susceptible Strain ◽  
Cotton Bollworm ◽  
Strong Positive Correlation ◽  
P450 Monooxygenase ◽  
Monooxygenase Activity ◽  
Helicoverpa Armígera

Abstract The role of cytochrome P450 monooxygenase in pyrethroid resistance was studied in different strains of the cotton bollworm, Helicoverpa armigera, from India. Filed collected strains of Nagpur and Delhi were compared to the laboratory reared population. The results showed a high resistance to deltamethrin, α-cypermethrin, and β-cyfluthrin. The results also showed that this resistance could be reduced by using piperonyl butoxide (PBO). The Nagpur and Delhi strains were found to have a 2.40 and 1.79 fold higher monooxygenase activity compared to a susceptible strain. A strong, positive correlation between monooxygenase activity and pyrethroid resistance was observed (r = 0.86 - 0.98). The relative expression of the housekeeping gene, EF-1α, and three P450 genes, was studied in the 5th instar larval midgut of the three strains. Out of the three P450 genes examined, expression of CYP6B7 mRNA was not detected in the midgut of the susceptible strain though it was highly expressed in the resistant strains. The midgut of the Nagpur strain had a 2.60 fold overexpression of CYP6B7 mRNA compared to the moderately resistant, Delhi strain. The mRNA of CYP4G8 and CYP6B2 were not overexpressed in either the Nagpur or Delhi strain. The results indicated that the elevated cytochrome P450 monooxygenase activity is associated with pyrethroid resistance in Indian strains of H. armigera, and CYP6B7 could be the P450 form responsible for pyrethroid resistance.

Identification and expression patterns of Halloween genes encoding cytochrome P450s involved in ecdysteroid biosynthesis in the cotton bollworm Helicoverpa armigera

2016 ◽  
Vol 107 (1) ◽  
pp. 85-95 ◽  
Author(s):  
J. Zheng ◽  
K. Tian ◽  
Y. Yuan ◽  
M. Li ◽  
X. Qiu
Keyword(s):  
Cytochrome P450 ◽  
Helicoverpa Armigera ◽  
Expression Patterns ◽  
Cotton Bollworm ◽  
Molecular Entity ◽  
Cytochrome P450 Enzymes ◽  
Steroid Biosynthesis ◽  
Ecdysteroid Biosynthesis ◽  
Helicoverpa Armígera ◽  
Specific Regulation

Abstract20-Hydroxyecdysone (20E) is a key hormone which regulates growth, development and reproduction in insects. Although cytochrome P450 enzymes (P450s) participating in the ecdysteroid biosynthesis of 20E have been characterized in a few model insects, no work has been published on the molecular entity of their orthologs in the cotton bollworm Helicoverpa armigera, a major pest insect in agriculture worldwide. In this study, four cytochrome P450 homologs, namely HarmCYP302A1, HarmCYP306A1, HarmCYP314A1 and HarmCYP315A1 from H. armigera, were identified and evolutional conservation of these Halloween genes were revealed among lepidopteran. Expression analyses showed that HarmCYP302A1 and HarmCYP315A1 were predominantly expressed in larval prothoracic glands, whereas this predominance was not always observed for HarmCYP306A1 and CYP314A1. The expression patterns of Halloween genes indicate that the fat bodies may play an important role in the conversion of ecdysone into 20E in larval–larval molt and in larval–pupal metamorphosis, and raise the possibility that HarmCYP315A1 plays a role in tissue-specific regulation in the steroid biosynthesis in H. armigera. These findings represent the first identification and expression characterization of four steriodogenic P450 genes and provide the groundwork for future functional and evolutionary study of steroid biosynthesis in this agriculturally important pest.

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