scholarly journals Environmental RNAi-based reverse genetics in the predatory mite Neoseiulus californicus: Towards improved methods of biological control

2021 ◽  
pp. 104993
Author(s):  
Noureldin Abuelfadl Ghazy ◽  
Takeshi Suzuki
Keyword(s):  
Biological Control ◽  
Reverse Genetics ◽  
Predatory Mite ◽  
Neoseiulus Californicus ◽  
Improved Methods

scholarly journals Environmental RNAi-based reverse genetics in the predatory mite Neoseiulus californicus: towards improved methods of biological control

2021 ◽  
Author(s):  
Noureldin Abuelfadl Ghazy ◽  
Takeshi Suzuki
Keyword(s):  
Applied Research ◽  
Reverse Genetics ◽  
Adult Stage ◽  
Experimental System ◽  
Spider Mites ◽  
Predatory Mite ◽  
Neoseiulus Californicus ◽  
Endogenous Gene ◽  
Adult Females ◽  
Improved Methods

The predatory mite Neoseiulus californicus (McGregor) (Mesostigmata: Phytoseiidae) has been commercialized by manufacturers in the pest control industry and is used worldwide as a natural enemy of spider mites. However, because its genome has not been sequenced, reverse genetics techniques that could be used to analyze gene function have not been established. Here we partially sequenced the gene that encodes the vacuolar-type H+-ATPase (V-ATPase), an ATP-dependent proton pump, in N. californicus (NcVATPase) and then conducted a functional analysis using environmental RNA interference (eRNAi) by orally administering sequence-specific exogenous dsRNA (dsRNA-NcVATPase) to larvae and adult females. The larvae treated with dsRNA-NcVATPase took longer to develop and had lower survivorship, fecundity, and offspring viability at the adult stage than those treated with a control dsRNA. Adult females treated with dsRNA-NcVATPase showed significant reductions in survival, fecundity, and prey consumption, and their endogenous gene expression level of NcVATPase was reduced by approximately 65% compared with the control. Our findings suggest that the NcVATPase gene, silencing of which inhibits feeding and reproduction, is an excellent biomarker for investigating the eRNAi mechanism in N. californicus. The highly efficient experimental system of eRNAi established in this study paves the way for applied research using eRNAi to enhance the predatory ability of N. californicus.

scholarly journals Life history of Neoseiulus californicus (McGregor, 1954) (Acari: Phytoseiidae) fed with castor bean (Ricinus communisL.) pollen in laboratory conditions

2014 ◽  
Vol 74 (3) ◽  
pp. 691-697 ◽  
Author(s):  
PP Marafeli ◽  
PR Reis ◽  
EC. da Silveira ◽  
GC Souza-Pimentel ◽  
MA. de Toledo
Keyword(s):  
Life History ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Intrinsic Rate ◽  
Predatory Mite ◽  
Neoseiulus Californicus ◽  
Intrinsic Rate Of Increase ◽  
Ricinus Communis L ◽  
Rate Of Increase ◽  
History Of

The predatory mite, Neoseiulus californicus(McGregor, 1954) (Acari: Phytoseiidae) is one of the principal natural enemies of tetranychid mites in several countries, promoting efficient control of those mites in several food and ornamental crops. Pest attacks such as that of the spider mite, Tetranychus urticaeKoch, 1836 (Acari: Tetranychidae), is one of the problems faced by farmers, especially in the greenhouse, due to the difficulty of its control with the use of chemicals because of the development of fast resistance making it hard to control it. The objective of this work was to study the life history of the predatory mite N. californicus as a contribution to its mass laboratory rearing, having castor bean plant [Ricinus communis L. (Euphorbiaceae)] pollen as food, for its subsequent use as a natural enemy of T. urticae on a cultivation of greenhouse rosebushes. The studies were carried out in the laboratory, at 25 ± 2°C of temperature, 70 ± 10% RH and a 14 hour photophase. The biological aspects and the fertility life table were appraised. Longevity of 32.9 days was verified for adult females and 40.4 days for males. The intrinsic rate of increase (rm) was 0.2 and the mean generation time (T) was 17.2 days. The population doubled every 4.1 days. The results obtained were similar to those in which the predatory mite N. californicus fed on T. urticae.

Diverse non-crop vegetation assemblages as banker plants for predatory mites in strawberry crop

2021 ◽  
pp. 1-10
Author(s):  
Fernando Teruhiko Hata ◽  
Pedro Henrique Togni ◽  
Maurício Ursi Ventura ◽  
José Eduardo Poloni da Silva ◽  
Nilson Zacarias Ferreira ◽  
...  
Keyword(s):  
Biological Control ◽  
Predatory Mites ◽  
Predatory Mite ◽  
Plant Composition ◽  
Field Crops ◽  
Banker Plant ◽  
Solanum Americanum ◽  
Leonurus Sibiricus ◽  
Plant Inventory ◽  
Diverse Plant

Abstract Non-crop plant diversity plays a fundamental role in the conservation of predatory mite (PM) and can be proposed as a banker plant system (BPS). BPSs provide plants that host natural enemies in greenhouses or field crops and may improve the efficiency of biological control. The aim of this study was to investigate if a diverse plant composition could be a suitable BPS for PMs in strawberry crops. A plant inventory characterized 22 species of non-crop plants harboring PMs. The most abundant PMs, in decreasing order, were Neoseiulus californicus, Neoseiulus anonymus, Euseius citrifolius, and Euseius concordis. PMs were randomly distributed among plants. We also found specific associations of Phytoseiidae species and phytophagous or generalist mites on plants. Due to this, four species were deemed suitable as banker plants: Capsicum sp., Leonurus sibiricus, Solanum americanum, and Urochloa mutica. Moreover, these plants combined a high PMs density and a low occurrence or absence of pest-mites. This study suggests shifting the traditional view that BPSs are composed of a limited number of species to use plant assemblages. This contributes to both conservation and augmentative biological control.

Biological Control by Neoseiulus Californicus (Mcgregor) (Acari: Phytoseiidae)

2006 ◽  
pp. 303-304
Author(s):  
Norma E. Sánchez ◽  
Nancy M. Greco ◽  
Claudia V. Cédola
Keyword(s):  
Biological Control ◽  
Neoseiulus Californicus
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