The effects of hydrogen sulfide on electrical field stimulation-induced neurogenic contractile responses in isolated rabbit lower esophageal sphincter: Contribution of nitrergic and non-adrenergic non-cholinergic transmission

2016 ◽  
Vol 68 (6) ◽  
pp. 1350-1357
Author(s):  
Halil Kara ◽  
Fatma Isli ◽  
Gokce Sevim Ozturk Fincan ◽  
Seniz Yildirim ◽  
Sevim Ercan ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Lower Esophageal Sphincter ◽  
Electrical Field Stimulation ◽  
Field Stimulation ◽  
Cholinergic Transmission ◽  
Electrical Field ◽  
Contractile Responses ◽  
Esophageal Sphincter

Biphasic relaxation of the opossum lower esophageal sphincter: roles of NO ⋅, VIP, and CGRP

1999 ◽  
Vol 277 (3) ◽  
pp. G548-G554 ◽  
Author(s):  
Aliye Uc ◽  
S. T. Oh ◽  
Joseph A. Murray ◽  
Eugene Clark ◽  
Jeffrey L. Conklin
Keyword(s):  
Nitric Oxide ◽  
Lower Esophageal Sphincter ◽  
Vasoactive Intestinal Polypeptide ◽  
Electrical Field Stimulation ◽  
Field Stimulation ◽  
Electrical Field ◽  
Calcitonin Gene ◽  
Esophageal Sphincter ◽  
Cgrp Antagonist ◽  
Transverse Muscle

Vasoactive intestinal polypeptide (VIP) and nitric oxide (NO ⋅) are thought to mediate lower esophageal sphincter (LES) relaxation. Transverse muscle strips from the opossum LES were used to test this hypothesis. Electrical field stimulation (EFS) produced a biphasic LES relaxation: a rapid component during the stimulus was more prominent at lower stimulus frequencies, and a sustained component was more prominent at higher frequencies. N ω-nitro-l-arginine and hemoglobin inhibited the rapid component but affected the sustained component less. Exogenous VIP decreased LES tone. A number of purported VIP antagonists blocked neither VIP-induced nor EFS-induced relaxation of the LES. The calcitonin gene-related peptide (CGRP) antagonist CGRP-(8—37) did not alter EFS-induced LES relaxation. EFS-induced relaxation of opossum LES muscle is biphasic, and the initial, rapid component of the relaxation is mediated primarily by NO ⋅. The mediator of the sustained component was not identified.

scholarly journals Effects of 5-hydroxytryptamine Receptor on the Reaction of the Lower Esophageal Sphincter Under the Electrical Field Stimulation

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Wei Li ◽  
Hefei Li ◽  
Zhenqing Sun ◽  
Ben Li ◽  
Qiang Guo ◽  
...  
Keyword(s):  
Lower Esophageal Sphincter ◽  
Muscle Tension ◽  
Electrical Field Stimulation ◽  
Measurement Technology ◽  
Field Stimulation ◽  
Electrical Field ◽  
Receptor Agonists ◽  
Esophageal Sphincter ◽  
Systolic And Diastolic Function

In the first and second parts of this study, 5-hydroxytryptamine (5HT) receptors, including 5-HT3 and 5-HT4 with the highest expression level, were found in clasp and sling fibres of the lower esophageal sphincter (LES). Specific 5-HT3 and 5-HT4 receptor agonists can induce the contraction effect of clasp and sling fibres of the LES while specific 5-HT7 receptor agonists showed no effects. In the study of this part, the in-vitro muscle tension measurement technology and EFS methods were used to detect the effect of the selective 5-HT receptor antagonist on the clasp and sling fibres of the in-vitro LES under the electrical field stimulation (EFS), and further to ensure the effect of 5-HT receptor in the LES neuroregulatory pathway, and deeply explore the effect of 5-HT receptor in the systolic and diastolic function regulation of the LES.

Control of neurotransmission by prostaglandins in canine trachealis smooth muscle

1984 ◽  
Vol 57 (1) ◽  
pp. 129-134 ◽  
Author(s):  
E. H. Walters ◽  
P. M. O'Byrne ◽  
L. M. Fabbri ◽  
P. D. Graf ◽  
M. J. Holtzman ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Prostaglandin E2 ◽  
Electrical Field Stimulation ◽  
Tracheal Smooth Muscle ◽  
Dependent Manner ◽  
Field Stimulation ◽  
Electrical Field ◽  
Similar Time ◽  
Concentration Dependent Manner ◽  
Contractile Responses

Contractile responses of canine tracheal smooth muscle to electrical field stimulation diminished over a 2-h period of incubation. However, addition of indomethacin (10(-5) M) for a similar time not only prevented this inhibition of contractile response, but actually markedly increased the response to electrical field stimulation, suggesting that prostaglandins were responsible for the time-dependent inhibition. Measured prostaglandin E2 increased in the tissue bath over 2 h in control tissues. Addition of prostaglandin E2 to the tissue produced similar inhibition of contractile responses to electrical field stimulation in a concentration-dependent manner. In contrast, incubation alone, treatment with indomethacin, or addition of prostaglandin E2 had little, if any, effect on contractions induced by acetylcholine. We conclude that the release of prostaglandins from canine tracheal smooth muscle that occurs with time has a predominantly inhibitory effect on cholinergic neurotransmission at a prejunctional site.

Influence of Endothelial Nitric Oxide on Adrenergic Contractile Responses of Human Cerebral Arteries

1996 ◽  
Vol 16 (4) ◽  
pp. 623-628 ◽  
Author(s):  
Martín Aldasoro ◽  
Carmen Martínez ◽  
José M. Vila ◽  
Pascual Medina ◽  
Salvador Lluch
Keyword(s):  
Nitric Oxide ◽  
Contractile Response ◽  
Cerebral Arteries ◽  
Electrical Field Stimulation ◽  
Field Stimulation ◽  
Oxide Formation ◽  
Electrical Field ◽  
Contractile Responses ◽  
Nitric Oxide Formation ◽  
Endothelial Nitric Oxide

The present study was designed to investigate the influence of the endothelium and that of the L-arginine pathway on the contractile responses of isolated human cerebral arteries to electrical field stimulation (EFS) and norepinephrine. Rings of human middle cerebral artery were obtained during autopsy of 19 patients who had died 3–8 h before. EFS (1–8 Hz) induced frequency-dependent contractions that were abolished by tetrodotoxin, prazosin, and guanethidine (all at 10-6 M). The increases in tension were of greater magnitude in arteries denuded of endothelium. NG-monomethyl L-arginine (L-NMMA 10-4 M) potentiated the contractile response to EFS in artery rings with endothelium but did not influence responses of endothelium-denuded arteries. L-arginine (10-4 M) reversed the potentiating effects of L-NMMA on EFS-induced contractions. Norepinephrine induced concentration-dependent contractions, which were similar in arteries with and without endothelium or in arteries treated with L-NMMA. Indomethacin (3 × 10−6 M) had no significant effect on the contractile response to EFS or on the inhibition by L-NMMA of acetylcholine-induced relaxation. These results suggest that the contractile response of human cerebral arteries to EFS is modulated by nitric oxide mainly derived from endothelial cells; although adrenergic nerves appear to be responsible for the contraction, the transmitter involved in the release of nitric oxide does not appear to be norepinephrine. The effects of L-NMMA in this preparation appear to be due to inhibition of nitric oxide formation rather than caused by cyclooxygenase activation.

Sign in / Sign up

Export Citation Format

Share Document