scholarly journals Direct mass measurement of bare short-lived 44V, 48Mn, 41Ti and 45Cr ions with isochronous mass spectrometry

2004 ◽  
Vol 586 (1-2) ◽  
pp. 27-33 ◽  
Author(s):  
J Stadlmann ◽  
M Hausmann ◽  
F Attallah ◽  
K Beckert ◽  
P Beller ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Measurement ◽  
Direct Mass Measurement ◽  
Isochronous Mass Spectrometry

Detection and identification of Catalpol metabolites in the rat plasma, urine and faeces using ultra-high performance liquid chromatography-Q Exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry

2020 ◽  
Vol 21 ◽  
Author(s):  
Zedong Xiang ◽  
Shaoping Wang ◽  
Haoran Li ◽  
Pingping Dong ◽  
Fan Dong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Performance ◽  
Mass Measurement ◽  
Neutral Loss ◽  
Rat Plasma ◽  
Accurate Mass ◽  
Chromatographic Retention ◽  
Rat Urine ◽  
Q Exactive

Background:: Catalpol, an iridoid glycoside, is one of the richest bioactive components present in Rehmannia glutinosa. More and more metabolites of drugs have exhibit various pharmacological effects, thus providing guidance for clinical application. However, few researches have paid attention on the metabolism of catalpol. Objective:: This study aimed to establish a rapid and effective method to identify catalpol metabolites and evaluate the biotransformation pathways of catalpol in rats. Methods:: In this study, catalpol metabolites in rat urine, plasma and faeces were analyzed by UHPLC-Q-Exactive MS for the characterization of metabolism of catalpol. Based on high-resolution extracted ion chromatograms (HREICs) and parallel reaction monitoring mode (PRM), metabolites of catalpol were identified by comparing the diagnostic product ions (DPIs), chromatographic retention times, neutral loss fragments (NLFs) and accurate mass measurement with those of catalpol reference standard. Results: A total of 29 catalpol metabolites were detected and identified in both negative and positive ion modes. Nine metabolic reactions including deglycosylation, hydroxylation, dihydroxylation, hydrogenation, dehydrogenation, oxidation of methylene to ketone, glucuronidation, glycine conjugation and cysteine conjugation were proposed. Conclusion:: A rapid and effective method based on UHPLC-Q-Exactive MS was developed to mine the metabolism information of catalpol. Results of metabolites and biotransformation pathways of catalpol suggested that when orally administrated, catalpol was firstly metabolized into catalpol aglycone, after which phase Ⅰ and phase Ⅱ reactions occurred. However, hydrophilic chromatography-mass spectrometry still needed to further find the polar metabolites of catalpol.

Study of projectile fragmentation reaction with isochronous mass spectrometry

2015 ◽  
Vol T166 ◽  
pp. 014009 ◽  
Author(s):  
X L Tu ◽  
B Mei ◽  
Y H Zhang ◽  
H S Xu ◽  
Yu A Litvinov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Projectile Fragmentation ◽  
Fragmentation Reaction ◽  
Isochronous Mass Spectrometry

Multiply Charged Cation Attachment to Facilitate Mass Measurement in Negative-Mode Native Mass Spectrometry

2022 ◽  
Author(s):  
Anthony M. Pitts-McCoy ◽  
Abdirahman M. Abdillahi ◽  
Kenneth W. Lee ◽  
Scott A. McLuckey
Keyword(s):  
Mass Spectrometry ◽  
Mass Measurement ◽  
Native Mass Spectrometry ◽  
Negative Mode ◽  
Multiply Charged
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