Molecular characterization and evaluation of the antibacterial activity of a plant defensin peptide derived from a gene of oat (Avena sativa L.)

2021 ◽  
Vol 181 ◽  
pp. 112586
Author(s):  
Sara Emamifar ◽  
Shamsozoha Abolmaali ◽  
Seyyed Mohsen Sohrabi ◽  
Mohsen Mohammadi ◽  
Maasume Shahmohammadi
Keyword(s):  
Antibacterial Activity ◽  
Molecular Characterization ◽  
Avena Sativa ◽  
Plant Defensin

scholarly journals Plant defensin antibacterial mode of action against Pseudomonas species

2020 ◽  
Author(s):  
Andrew Edward Sathoff ◽  
Shawn Lewenza ◽  
Deborah A. Samac
Keyword(s):  
Gene Expression ◽  
Antibacterial Activity ◽  
Outer Membrane ◽  
Pseudomonas Syringae ◽  
Mode Of Action ◽  
Fluorescent Microscopy ◽  
Insertion Site ◽  
Membrane Damage ◽  
Plant Defensin ◽  
Plant Defensins

Abstract Background: Though many plant defensins exhibit antibacterial activity, little is known about their antibacterial mode of action (MOA). Antimicrobial peptides with a characterized MOA induce the expression of multiple bacterial outer membrane modifications, which are required for resistance to these membrane-targeting peptides. Mini-Tn5-lux mutant strains of Pseudomonas aeruginosa with Tn insertions disrupting outer membrane protective modifications were assessed for sensitivity against plant defensin peptides. These transcriptional lux reporter strains were also evaluated for lux gene expression in response to sublethal plant defensin exposure. Also, a plant pathogen, Pseudomonas syringae pv. syringae was modified through transposon mutagenesis to create mutants that are resistant to in vitro MtDef4 treatments.Results: Plant defensins displayed specific and potent antibacterial activity against strains of P. aeruginosa. A defensin from Medicago truncatula, MtDef4, induced dose-dependent gene expression of the aminoarabinose modification of LPS and surface polycation spermidine production operons. The ability for MtDef4 to damage bacterial outer membranes was also verified visually through fluorescent microscopy. Another defensin from M. truncatula, MtDef5, failed to induce lux gene expression and limited outer membrane damage was detected with fluorescent microscopy. The transposon insertion site on MtDef4 resistant P. syringae pv. syringae mutants was sequenced, and modifications of ribosomal genes were identified to contribute to enhanced resistance to plant defensin treatments. Conclusions: MtDef4 damages the outer membrane similar to polymyxin B, which stimulates antimicrobial peptide resistance mechanisms to plant defensins. MtDef5, appears to have a different antibacterial MOA. Additionally, the MtDef4 antibacterial mode of action may also involve inhibition of translation.

scholarly journals Plant Defensin Peptides have Antifungal and Antibacterial Activity Against Human and Plant Pathogens

2019 ◽  
Vol 109 (3) ◽  
pp. 402-408 ◽  
Author(s):  
Andrew E. Sathoff ◽  
Siva Velivelli ◽  
Dilip M. Shah ◽  
Deborah A. Samac
Keyword(s):  
Antibacterial Activity ◽  
Plant Pathogens ◽  
Crown Rot ◽  
Plant Defensin ◽  
Core Motif ◽  
Potential Control ◽  
Fungal Plant Pathogens ◽  
Plant Defensins ◽  
Animal Pathogens

Plant defensins are small, cysteine-rich antimicrobial peptides. These peptides have previously been shown to primarily inhibit the growth of fungal plant pathogens. Plant defensins have a γ-core motif, defined as GXCX3-9C, which is required for their antifungal activity. To evaluate plant defensins as a potential control for a problematic agricultural disease (alfalfa crown rot), short, chemically synthesized peptides containing γ-core motif sequences were screened for activity against numerous crown rot pathogens. These peptides showed both antifungal and, surprisingly, antibacterial activity. Core motif peptides from Medicago truncatula defensins (MtDef4 and MtDef5) displayed high activity against both plant and human bacterial pathogens in vitro. Full-length defensins had higher antimicrobial activity compared with the peptides containing their predictive γ-core motifs. These results show the future promise for controlling a wide array of economically important fungal and bacterial plant pathogens through the transgenic expression of a plant defensin. They also suggest that plant defensins may be an untapped reservoir for development of therapeutic compounds for combating human and animal pathogens.

scholarly journals Evaluation of antibacterial activity and molecular characterization of bacteria from Holothuria atra intestine collected from anthropogenic and non-anthropogenic region in Karimunjawa, Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Bambang Sulardiono ◽  
NINIEK WIDYORINI ◽  
DJOKO SUPRAPTO ◽  
DIAH AYUNINGRUM ◽  
ARIF RAHMAN
Keyword(s):  
Antibacterial Activity ◽  
Molecular Characterization ◽  
Molecular Identification ◽  
Sea Cucumber ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Agar Plug ◽  
16S Rdna Pcr ◽  
Holothuria Atra

Abstract. Sulardiono B, Widyorini N, Suprapto D, Ayuningrum D, Rahman A. 2020. Evaluation of antibacterial activity and molecular characterization of bacteria from Holothuria atra intestine collected from anthropogenic and non-anthropogenic region in Karimunjawa, Indonesia. Biodiversitas 21: 3149-3155. Evaluation of antibacterial activity and molecular characterization of bacteria from the intestine of Holothuria atra is needed in the anthropogenic region of Menjangan Besar, Karimunjawa. The research aims to evaluate antibacterial activity and molecular characterization of bacteria from intestine of Holothuria atra collected from anthropogenic and non-anthropogenic regions in Karimunjawa, Indonesia. Sea cucumber samples were collected at Menjangan Besar waters as anthropogenic region (code of HM) and Alang-alang waters as non-anthropogenics region (code of HA), Karimunjawa National Park, Indonesia. The H. atra sample collection was using purposive sampling method. Examination of the bacteria from isolation until molecular characterisation was done at the Tropical Marine Biotechnology Laboratory, Uiversitas Diponegoro. The isolation process was conducted using spread plate method, followed by bacterial isolates purification using streak plate method. Screening of antibacterial activity using the agar plug method, as well as molecular identification was conducted by 16S rDNA PCR amplification. The results of this study indicated that a total of 26 bacterial strains were successfully isolated from sea cucumber intestine, consisted of eleven bacterial isolates from H. atra in Menjangan Besar waters and the rest fifteen isolates from H. atra in Alang-alang waters. The preliminary assay of antibacterial test showed H. atra in both waters have potential bacterial isolates. Those bacterial isolates with antibacterial activity were HM1.2 and HA1.1, which based on molecular identification, showed isolates HM1.2 and HA1.1 had the closest similarity with Bacillus paramcoides and Vibrio alginolyticus consecutively, with BLAST homology 98% and 99%. The accession number for both isolates were LC550090 and LC550089, respectively.

Molecular characterization of peptide fractions of a Tilapia (Oreochromis niloticus) by-product hydrolysate and in vitro evaluation of antibacterial activity

2015 ◽  
Vol 50 (3) ◽  
pp. 487-492 ◽  
Author(s):  
Marie Robert ◽  
Céline Zatylny-Gaudin ◽  
Vincent Fournier ◽  
Erwan Corre ◽  
Gildas Le Corguillé ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Molecular Characterization ◽  
Oreochromis Niloticus ◽  
In Vitro Evaluation ◽  
Peptide Fractions

Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum

2017 ◽  
Vol 65 ◽  
pp. 17-24 ◽  
Author(s):  
Dinglong Yang ◽  
Qing Wang ◽  
Lizhu Chen ◽  
Yongliang Liu ◽  
Ruiwen Cao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Molecular Characterization ◽  
Phage Type ◽  
Ruditapes Philippinarum ◽  
Manila Clam

scholarly journals Plant defensin antibacterial mode of action against Pseudomonas species

2020 ◽  
Author(s):  
Andrew E. Sathoff ◽  
Shawn Lewenza ◽  
Deborah A. Samac
Keyword(s):  
Gene Expression ◽  
Antibacterial Activity ◽  
Outer Membrane ◽  
Pseudomonas Syringae ◽  
Mode Of Action ◽  
Fluorescent Microscopy ◽  
Insertion Site ◽  
Membrane Damage ◽  
Plant Defensin ◽  
Plant Defensins

Abstract Background Though many plant defensins exhibit antibacterial activity, little is known about their antibacterial mode of action (MOA). Antimicrobial peptides with a characterized MOA induce the expression of multiple bacterial outer membrane modifications, which are required for resistance to these membrane-targeting peptides. Mini-Tn 5-lux mutant strains of Pseudomonas aeruginosa with Tn insertions disrupting outer membrane protective modifications were assessed for sensitivity against plant defensin peptides. These transcriptional lux reporter strains were also evaluated for lux gene expression in response to sublethal plant defensin exposure. Also, a plant pathogen, Pseudomonas syringae pv. syringae was modified through transposon mutagenesis to create mutants that are resistant to in vitro MtDef4 treatments. Results Plant defensins displayed specific and potent antibacterial activity against strains of P. aeruginosa . A defensin from Medicago truncatula , MtDef4, induced dose-dependent gene expression of the aminoarabinose modification of LPS and surface polycation spermidine production operons. The ability for MtDef4 to damage bacterial outer membranes was also verified visually through fluorescent microscopy. Another defensin from M. truncatula , MtDef5, failed to induce lux gene expression and limited outer membrane damage was detected with fluorescent microscopy. The transposon insertion site on MtDef4 resistant P. syringae pv. syringae mutants was sequenced, and modifications of ribosomal genes were identified to contribute to enhanced resistance to plant defensin treatments. Conclusions MtDef4 damages the outer membrane similar to polymyxin B, which stimulates antimicrobial peptide resistance mechanisms to plant defensins. MtDef5, appears to have a different antibacterial MOA. Additionally, the MtDef4 antibacterial mode of action may also involve inhibition of translation.

Molecular characterization of a novel hepcidin (HepcD) from Camelus dromedarius . Synthetic peptide forms exhibit antibacterial activity

2014 ◽  
Vol 20 (9) ◽  
pp. 680-688 ◽  
Author(s):  
Mohamed Boumaiza ◽  
Aymen Ezzine ◽  
Maryse Jaouen ◽  
Marie-Agnes Sari ◽  
Mohamed Nejib Marzouki
Keyword(s):  
Antibacterial Activity ◽  
Molecular Characterization ◽  
Synthetic Peptide ◽  
Camelus Dromedarius

scholarly journals Plant defensin antibacterial mode of action against Pseudomonas species

2020 ◽  
Author(s):  
Andrew E. Sathoff ◽  
Shawn Lewenza ◽  
Deborah A. Samac
Keyword(s):  
Gene Expression ◽  
Antibacterial Activity ◽  
Outer Membrane ◽  
Pseudomonas Syringae ◽  
Mode Of Action ◽  
Fluorescent Microscopy ◽  
Insertion Site ◽  
Membrane Damage ◽  
Plant Defensin ◽  
Plant Defensins

Abstract Background: Though many plant defensins exhibit antibacterial activity, little is known about their antibacterial mode of action (MOA). Antimicrobial peptides with a characterized MOA induce the expression of multiple bacterial outer membrane modifications, which are required for resistance to these membrane-targeting peptides. Mini-Tn 5-lux mutant strains of Pseudomonas aeruginosa with Tn insertions disrupting outer membrane protective modifications were assessed for sensitivity against plant defensin peptides. These transcriptional lux reporter strains were also evaluated for lux gene expression in response to sublethal plant defensin exposure. Also, a plant pathogen, Pseudomonas syringae pv. syringae was modified through transposon mutagenesis to create mutants that are resistant to in vitro MtDef4 treatments. Results: Plant defensins displayed specific and potent antibacterial activity against strains of P. aeruginosa . A defensin from Medicago truncatula , MtDef4, induced dose-dependent gene expression of the aminoarabinose modification of LPS and surface polycation spermidine production operons. The ability for MtDef4 to damage bacterial outer membranes was also verified visually through fluorescent microscopy. Another defensin from M. truncatula , MtDef5, failed to induce lux gene expression and limited outer membrane damage was detected with fluorescent microscopy. The transposon insertion site on MtDef4 resistant P. syringae pv. syringae mutants was sequenced, and modifications of ribosomal genes were identified to contribute to enhanced resistance to plant defensin treatments. Conclusions: MtDef4 damages the outer membrane similar to polymyxin B, which stimulates antimicrobial peptide resistance mechanisms to plant defensins. MtDef5, appears to have a different antibacterial MOA. Additionally, the MtDef4 antibacterial mode of action may also involve inhibition of translation.

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