AlthoughR. glutinosaroots are currently the only organ source in clinics, its leaves are a potential supplement for the roots especially in extraction of some important bioactive compounds. Our early work found that the contents of catalpol and total iridoid glycosides varied among different developmental stages ofR. glutinosaleaves. Aucubin and geniposidic acid, the abundant major bioactive compounds inEucommia ulmoidesandGardenia jasminoides, respectively, were found present inR. glutinosaroots, however, and have not been analyzed in its leaves. In this paper, we aimed to determine contents of these three iridoid glycosides in different developmental stages ofR. glutinosaleaves using the optimized HPLC-UV conditions. Our results showed that aucubin and GPA inR. glutinosaleaves were much lower than catalpol and showed the increasing trend with the leaf development, which was different from catalpol. This work provided the important information for future exploitation ofR. glutinosaleaves as a potential supplement for its roots in extraction of some important bioactive compounds and studying the relationship of aucubin and catalpol metabolism.
Thermospray Liquid Chromatographic/Mass Spectrometric Analysis of Iridoid Glycosides from Gardenia jasminoides.
