Molecular cloning and characterization of the genes encoding an auxin efflux carrier and the auxin influx carriers associated with the adventitious root formation in mango (Mangifera indica L.) cotyledon segments

2012 ◽  
Vol 55 ◽  
pp. 33-42 ◽  
Author(s):  
Yun-He Li ◽  
Ming-Hong Zou ◽  
Bi-Hong Feng ◽  
Xia Huang ◽  
Zhi Zhang ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Adventitious Root ◽  
Root Formation ◽  
Mangifera Indica ◽  
Adventitious Root Formation ◽  
Genes Encoding ◽  
Auxin Efflux Carrier

scholarly journals Molecular Cloning and Characterization of Auxin-regulated Genes from Mungbean Hypocotyls during Adventitious Root Formation

1996 ◽  
Vol 121 (3) ◽  
pp. 393-398 ◽  
Author(s):  
Jun Chen ◽  
Dengru Wu ◽  
Francis H. Witham ◽  
Charles W. Heuser ◽  
Richard N. Arteca
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Basal Expression ◽  
Molecular Events ◽  
Expression Studies ◽  
Genes Expression ◽  
High Degree ◽  
Indole 3 Acetic Acid

Adventitious root formation (rooting) in `Berken' mungbean [Vigna radiata (L.) Rwiclz.] cuttings is stimulated by indole-3-acetic acid (IAA). To understand the molecular events that occur during IAA-induced adventitious root initiation, a λgt11 cDNA library was made from mungbean hypocotyls treated with 500 μm IAA for 3 hours and differentially screened. Two cDNAs MII-3 and MII-4 were isolated. Southern analysis revealed that both cDNAs are encoded by different genes. Expression studies showed different patterns for both genes. Both MII-3 and MII-4 were highly expressed in IAA treated hypocotyls, whereas MII-4 was also induced in IAA treated epicotyls. There was no expression of either MII-3 or MII-4 in control or IAA treated leaves. With increasing concentrations of IAA from 100 to 1000 μm there was an increase in the average root number per cutting as well as a stimulation in MII-3 and MII-4. Both MII-3 and MII-4 showed a stimulation in expression 4 hours following treatment with 500 μm IAA reaching a maximum from 4 to 8 hours followed by a decline thereafter. Basal expression of MII-3 was evident between 2 and 8 hours, whereas, a high degree of basal expression was found with MII-4 from 1 to 8 hours followed by a sharp decline. Cycloheximide (50 μm) dramatically reduced rooting and MII-3 expression, whereas MII-4 was only slightly affected.

Histological and Biochemical Characterization of Adventitious Root Formation in Walnut Cotyledon Fragments

1997 ◽  
pp. 75-84 ◽  
Author(s):  
Laurent Duroux ◽  
Fabienne Fontaine ◽  
Christian Breton ◽  
Jean-Paul Charpentier ◽  
Patrick Doumas ◽  
...  
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Biochemical Characterization ◽  
Adventitious Root Formation
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