A novel formaldehyde metabolic pathway plays an important role during formaldehyde metabolism and detoxification in tobacco leaves under liquid formaldehyde stress

Low temperature x-ray microanalysis (LTXM) of solid biological materials has been documented for Lemna minor L. root tips. This discussion will be limited to a demonstration of LTXM for measuring relative elemental distributions of P,S,Cl and K species within whole cells of tobacco leaves.Mature Wisconsin-38 tobacco was grown in the greenhouse at the University of California, Berkeley and picked daily from the mid-stalk position (leaf #9). The tissue was excised from the right of the mid rib and rapidly frozen in liquid nitrogen slush. It was then placed into an Amray biochamber and maintained at 103K. Fracture faces of the tissue were prepared and carbon-coated in the biochamber. The prepared sample was transferred from the biochamber to the Amray 1000A SEM equipped with a cold stage to maintain low temperatures at 103K. Analyses were performed using a tungsten source with accelerating voltages of 17.5 to 20 KV and beam currents from 1-2nA.

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Simultaneous HPLC determination of four key metabolites in the metabolic pathway for production of 1,3-propanediol from glycerol

Chromatographia ◽

10.1016/s0009-5893(07)82072-8 ◽

2007 ◽

Vol 65 (9-10) ◽

pp. 629-632

Author(s):

H CHEN ◽

B FANG ◽

Z HU

Keyword(s):

Metabolic Pathway ◽

Hplc Determination

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Glucose regulation of secretory and metabolic pathway genes in human pancreatic islets

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-2005-862882 ◽

2005 ◽

Vol 113 (S 1) ◽

Author(s):

BJ Manfras ◽

P Hengster ◽

BO Böhm

Keyword(s):

Pancreatic Islets ◽

Metabolic Pathway ◽

Glucose Regulation ◽

Human Pancreatic Islets

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PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

Jurnal Rekayasa Lingkungan ◽

10.29122/jrl.v5i1.1875 ◽

2018 ◽

Vol 5 (1) ◽

Author(s):

Mahyudin Abdul Rachman

Keyword(s):

Metabolic Pathway ◽

Acid Production ◽

Double Mutant ◽

Enterobacter Aerogenes ◽

H2 Production ◽

Hydrogen Gas ◽

Survival Ratio ◽

Ethyl Methane Sulfonate ◽

Methane Sulfonate ◽

Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

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