PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Mahyudin Abdul Rachman
Keyword(s):  
Metabolic Pathway ◽  
Acid Production ◽  
Double Mutant ◽  
Enterobacter Aerogenes ◽  
H2 Production ◽  
Hydrogen Gas ◽  
Survival Ratio ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Mahyudin Abdul Rachman
Keyword(s):  
Metabolic Pathway ◽  
Acid Production ◽  
Double Mutant ◽  
Enterobacter Aerogenes ◽  
H2 Production ◽  
Hydrogen Gas ◽  
Survival Ratio ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12,13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase defficient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methanesludge

scholarly journals PENINGKATAN KAPASITAS PRODUKSI GAS HIDROGEN (H2) DENGAN SUBSTRAT LIMBAH BIODIESEL OLEH MUTAN GANDA Enterobacter aerogenes AD-H43 DI BATCH STIRRED TANK REACTOR (BSTR)

2019 ◽  
Vol 6 (1) ◽  
pp. 21
Author(s):  
Iwan Hidayat ◽  
Mahyudin A.R. ◽  
Srikandi Srikandi
Keyword(s):  
Lactic Acid ◽  
Enterobacter Aerogenes ◽  
Hydrogen Gas ◽  
Stirred Tank ◽  
Chemical Physics ◽  
Ethyl Methane Sulfonate ◽  
Hydrogen Atoms ◽  
Methane Sulfonate ◽  
Ethyl Methane ◽  
Tank Reactor

Enhancement of Hydrogen Gas Production Capacity (H2) With Substrate of Biodiesel Waste By Double Multiles Enterobacter aerogenes Ad-H43 In Batch Stirred Tank Reactor (Bstr)Hydrogen is the simplest element consisting of only one proton and one electron. Almost all components inside the cell contain hydrogen atoms. Hydrogen gas (H2) consists of two binding hydrogen atoms. H2 can be producted by chemical / physics method and biological method. The production of H2 by chemical / physics method is done thermochemically and electrolyzed water, while biologically done by microorganisms through direct and indirect biofotolysis as well as light and dark fermentation. The results showed that H2 production using a double Enterobacter aerogenes AD-H43 mutant on the BSTR fermentor scale occurred an increase in H2 capacity followed by decreased production of lactic acid due to mutation with Ethyl Methane Sulfonate (EMS). On the glycerol substrate E. aerogenes AD-H43 produces H2 of 3.14 mol / mol glycerol while E. aerogenes AY-2 produces only H2 of 2.65 mol / mol glycerol, or an increase of 18% compared to E. aerogenes AY-2 whereas for production lactic acid decreased 33% while in biodiesel waste E. aerogenes AD-H43 yield H2 0.98 mol / mol glycerol and E. aerogenes AY-2 only 0.85 mol / mol glycerol or about 15% increase and in purified biodiesel waste resulting in a higher yield of H2 from biodiesel waste of 0.89 mol / mol glycerol at E. aerogenes AY-2 and 0.98 mol / mol glycerol in E. aerogenes AD-H43.Keywords: Hydrogen, Enterobacter aerogenes, BSTR fermentorABSTRAKHidrogen adalah unsur paling sederhana yang hanya terdiri dari satu proton dan satu elektron.  Hampir semua komponen di dalam sel mengandung atom hidrogen.  Gas hidrogen (H2) terdiri atas dua atom hidrogen yang berikatan.  Pembuatan H2 dapat dilakukan dengan metode kimia/fisika dan metode biologis.  Produksi H2 dengan metode kimia/fisika dilakukan secara termokimia dan elektrolisis air, sedangkan secara biologis dilakukan oleh mikroorganisme melalui biofotolisis langsung dan tidak langsung serta fermentasi terang dan gelap. Hasil penelitian menunjukkan bahwa produksi H2 menggunakan mutan ganda Enterobacter aerogenes AD-H43 pada skala fermentor BSTR terjadi peningkatan kapasitas H2 dengan diikuti penurunan produksi asam laktat akibat mutasi dengan Ethyl Methane Sulfonate (EMS). Pada substrat gliserol E. aerogenes AD-H43 memproduksi H2 sebesar 3.14 mol/mol gliserol sedangkan E. aerogenes AY-2 hanya  memproduksi H2 sebesar 2.65 mol/mol gliserol,atau mengalami kenaikan sebesar 18 % dibandingkan E. aerogenes AY-2 sedangkan untuk produksi asam laktatnya terjadi penurunan 33% sedangkan pada limbah biodiesel E. aerogenes AD- H43 menghasilkan  yield H2 0.98 mol/mol gliserol dan E. aerogenes AY-2 hanya 0.85 mol/mol gliserol atau terjadi kenaikan sekitar 15 % dan pada limbah biodiesel yang di purifikasi menghasilkan yield H2 yang lebih tinggi dari limbah biodiesel yaitu 0.89 mol/mol gliserol pada E. aerogenes AY-2 dan 0.98 mol/mol gliserol  pada E. aerogenes AD-H43. Kata Kunci: Hidrogen, Enterobacter aerogenes, fermentor BSTR

Effectiveness and efficiency of electron beam in comparison with gamma rays and ethyl methane sulfonate mutagens in cowpea

2021 ◽  
Vol 171 ◽  
pp. 109640
Author(s):  
Vijayakumar Eswaramoorthy ◽  
Thangaraj Kandasamy ◽  
Kalaimagal Thiyagarajan ◽  
Chockalingam Vanniarajan ◽  
Souframanien Jegadeesan
Keyword(s):  
Electron Beam ◽  
Gamma Rays ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane ◽  
Effectiveness And Efficiency

scholarly journals EXTRACHROMOSOMAL ELEMENT DELTA IN DROSOPHILA MELANOGASTER. IX. INDUCTION OF DELTA-RETAINING CHROMOSOME LINES BY MUTATION AND GENE MAPPING

Genetics ◽  
1973 ◽  
Vol 74 (3) ◽  
pp. 477-487
Author(s):  
Sumio Minamori ◽  
Kinue Sugimoto
Keyword(s):  
Drosophila Melanogaster ◽  
Crossing Over ◽  
Chromosomal Gene ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Multiple Alleles ◽  
Ethyl Methane ◽  
Extrachromosomal Element ◽  
Polygenic System ◽  
Killing Action

ABSTRACT [Delta b], symbolized as [δb], is retained by Sb chromosome lines and transmitted through the females to their progeny. Transmission through the males is not directly demonstrable (Minamori 1969a). [delta r], symbolized as [δr], is retained by Sr chromosome lines and transmitted biparentally (Minamori 1971). The multiplication of delta is suppressed at low temperature. All descendant lines derived from Sb-carrying or Sr-carrying flies in which the presence of delta cannot be demonstrated gradually accumulate their specific delta factors over many generations (Minamori 1969b, 1972). The delta factors and the sensitive chromosomes are inseparably associated. This observation led to the assumption that delta may be a copy of a chromosomal gene or a certain agent integrated into the chromosome (Minamori 1972). This assumption was examined in the present study by experiments designed to induce delta-retaining sensitive chromosomes, and to map the gene(s) responsible for delta-retention and/or for sensitivity to the killing action of delta factor. One sensitive chromosome which retained [δb] (Sb chromosome) was obtained in the presence of [δb] out of 2492 insensitive chromosomes which retained no delta; in addition one Sb chromosome was obtained in the presence of [δr] out of 2131 insensitives. The latter finding suggests that Sb might be induced by a mutation caused by [δb] or [δr], but not by integration of either delta into the chromosome. Four Sb chromosomes and one sensitive chromosome which retained [δr] (Sr chromosome) were obtained out of 1970 insensitives when males carrying the chromosome were fed an alkylating mutagen, ethyl methane sulfonate (EMS). The location of delta-retaining genes was examined by crossing-over experiments employing eight Sb and five Sr chromosomes. The genes on these chromosomes were found to be located in the same region or near one another. The gene for [δb], symbolized as Dab, and the gene for [δr], symbolized as Dar, are assumed to be multiple alleles of a locus at 2-24.9. The sensitivity of the chromosomes was modified appreciably by recombination; hence, the genes controlling this trait are assumed to be a polygenic system. The findings obtained in this study lead to the hypothesis that delta may be produced by a chromosomal gene (Da) and transmitted extrachromosomally.

Enhanced production of extracellular lipase by ethyl methane sulfonate from soil fungal strain in submerged and solid-state fermentation condition

2021 ◽  
Vol 16 (7) ◽  
pp. 64-70
Author(s):  
Priya Chaudhary ◽  
Arun Kumar Sharma ◽  
Pracheta Janmeda
Keyword(s):  
Solid State ◽  
Enzymatic Activity ◽  
Solid State Fermentation ◽  
Wild Strain ◽  
Fungal Strain ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Random Mutation ◽  
Ethyl Methane ◽  
Time Period

Enhancement in the production of enzyme by utilizing different strains of microbe is one of the main prospects in biotechnology. In the present work, ethyl methane sulfonate (EMF) was selected as the chemical mutagen for inducing mutagenesis in fungi. It is a cheap method to induce random mutation as compared to other methods of recombinant technologies. Strain improvement was done by incubating the fungal spore suspension at variable concentrations of EMS i.e. 4% (v/v) and 10% (v/v) for the time period of 60, 90, and 120 min respectively. The set of control was treated with distilled water only. The fungal colonies were found to be maximum in control plate as compared to the EMF exposed plates. The number of fungal colonies was reduced as we raised the exposure time of EMF. Specific activity and the lipase activity of wild strain and hyperproducer were evaluated under the submerged (SmF) and solid-state fermentation (SSF). The wild strain denoted the 3.2 U/ml/min of enzymatic activity under SmF and 15.87 U/g/min of activity under SSF. In contrast, the best enzymatic activity was represented by S2St1 at 10% of EMS after the time period of 60 min i.e. 11.7 U/ml/min under SmF and 99.35 U/g/min under SSF after the time period of 72 hrs. Statistical analysis by using one-way ANOVA determined that the value of F calculated was lower than the F tabulated. So, there was a significant relation between the EMS percentage and time of exposure among the mutated strains. In conclusion, this soil fungal strain can be utilized to produce lipase enzyme for numerous industrial applications.

scholarly journals Curly Stem – an Induced Mutation in Flax (Linum usitatissimum L.)

2012 ◽  
Vol 38 (No. 3-4) ◽  
pp. 125-128 ◽  
Author(s):  
E. Tejklová
Keyword(s):  
Linum Usitatissimum ◽  
Wild Type Allele ◽  
Ethyl Methane Sulfonate ◽  
Wild Type ◽  
Methane Sulfonate ◽  
Ethyl Methane ◽  
Straight Stem ◽  
Linum Usitatissimum L ◽  
Factors Influencing ◽  
Stem Shape

After ethyl methane sulfonate (EMS) treatment of two flax lines, curly stem mutations appeared in both, besides of other mutations. Genetic analysis of one CS mutant line confirmed a monogenic inheritance of the changed stem shape. The curly stem allele is partially dominant over the wild type allele for straight stem. Homozygotic mutants have a curly stem, heterozygotic plants have a flexuous stem, while the stem of homozygotic recessive plants is straight. The expression of the curly stem character is affected by factors influencing plant growth. The utilisation of this mutation for ornamental and other purposes is considered.

scholarly journals Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia

2001 ◽  
Vol 43 (6) ◽  
Author(s):  
Carlos Alvarez-Moya ◽  
Anne Santerre-Lucas ◽  
Guillermo Zúñiga-González ◽  
Olivia Torres-Bugarín ◽  
Eduardo Padilla-Camberos ◽  
...  
Keyword(s):  
Maleic Hydrazide ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane ◽  
Genotoxic Activity

scholarly journals Registration of six upland cotton germplasm lines with improved fiber quality through ethyl methane sulfonate treatments and selection

2020 ◽  
Vol 14 (2) ◽  
pp. 159-164
Author(s):  
Efrem Bechere ◽  
Dick L. Auld ◽  
C. Wayne Smith ◽  
Roy G. Cantrell ◽  
Eric F. Hequet ◽  
...  
Keyword(s):  
Upland Cotton ◽  
Fiber Quality ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane
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