Differential phosphatidic acid metabolism in barley leaves and roots induced by chilling temperature

2018 ◽  
Vol 132 ◽  
pp. 174-182 ◽  
Author(s):  
Micaela Peppino Margutti ◽  
Virginia L. Gaveglio ◽  
Matias Reyna ◽  
Susana J. Pasquaré ◽  
Graciela E. Racagni ◽  
...  
2019 ◽  
Vol 10 ◽  
Author(s):  
Karel Klem ◽  
Albert Gargallo-Garriga ◽  
Wutthida Rattanapichai ◽  
Michal Oravec ◽  
Petr Holub ◽  
...  

1987 ◽  
Author(s):  
J M Cascar ◽  
J L Kavarro

Arachidonlc acid (AA) netabolisn has been extensively studied in fresh platelets, but there Is little infomation available for stored platelets . we stored platelets in CLI bags far five days at 22+/-2°C and, on days 0, 3 and 5, six si of platelet concentrate were revved from the container and platelets were labeled with (C-14J-AA. Both incorporation and distribution of radiotracer sere studied in rest and thrcnfcln stivlated platelets.Total uptake of, (C-14)-AA dropped fron day 0 to 5 (p 0.01). Distribution oh day 0 was sinilar to fresh platelets. Incorporation of (C-14)-AA on phosphatidyl inositol (FI) decayed fron 12.4+/-1.5 on day 0, to 7.9+/-0.9 on day 3 (p 0.001), While the percentage attached on phosphatidylserine (PS),increased fron 5.3+/-0.9 to 8.8+/-1.5 (p 0.001). There were not any changes fron day 3 to 5.On day 0,17.7+/-5.2X of radiactlvity was released fron phospholipids by thronbin. This anount decreased to 7.3+/-2.5X (p 0.01) on day 5. I^xaiment in breakdown of both PI and phosphatidylcholine (PC) was detected. Generation of phosphatidic acid (PA) by thronbin, decreased fron 2.6+/-0.4X of total radiactlvity on day 0 to 1.4+/-0.3X on day 3 (p 0.001) and 0.9+/-0.2X on day 5 (p 0.01). Ve did not find changes in TxB2 and HHT, but HETE decayed fron 7.2+/-2.9X on day 0, to 2.3t/-0.9% on day 5 (p 0.01).We concluded that both activities of phospholipases A-2 and C are affected by storage.


2012 ◽  
Vol 133 ◽  
pp. 40-46 ◽  
Author(s):  
Lin-Tong Yang ◽  
Li-Song Chen ◽  
Hao-Yang Peng ◽  
Peng Guo ◽  
Ping Wang ◽  
...  

2011 ◽  
Vol 2011 ◽  
pp. 1-18 ◽  
Author(s):  
Susana J. Pasquaré ◽  
Virginia L. Gaveglio ◽  
Norma M. Giusto

This paper explores the way ceramide, sphingosine, ceramide 1-phosphate, and sphingosine 1-phosphate modulate the generation of second lipid messengers from phosphatidic acid in two experimental models of the central nervous system: in vertebrate rod outer segments prepared from dark-adapted retinas as well as in rod outer segments prepared from light-adapted retinas and in rat cerebral cortex synaptosomes under physiological aging conditions. Particular attention is paid to lipid phosphate phosphatase, diacylglycerol lipase, and monoacylglycerol lipase. Based on the findings reported in this paper, it can be concluded that proteins related to phototransduction phenomena are involved in the effects derived from sphingosine 1-phosphate/sphingosine or ceramide 1-phosphate/ceramide and that age-related changes occur in the metabolism of phosphatidic acid from cerebral cortex synaptosomes in the presence of either sphingosine 1-phosphate/sphingosine or ceramide 1-phosphate/ceramide. The present paper demonstrates, in two different models of central nervous system, how sphingolipids influence phosphatidic acid metabolism under different physiological conditions such as light and aging.


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