Characterization and functional analysis of LoUDT1, a bHLH transcription factor related to anther development in the lily oriental hybrid Siberia (Lilium spp.)

2021 ◽  
Author(s):  
Guozhen Yuan ◽  
Ze Wu ◽  
Xinyue Liu ◽  
Ting Li ◽  
Nianjun Teng
Keyword(s):  
Functional Analysis ◽  
Transcription Factor ◽  
Anther Development ◽  
Bhlh Transcription Factor

Characterization and Functional Analysis of Transcription Factor LoMYB80 Related to Anther Development in Lily (Lilium Oriental Hybrids)

2015 ◽  
Vol 34 (3) ◽  
pp. 545-557 ◽  
Author(s):  
Juanjuan Sui ◽  
Junna He ◽  
Jian Wu ◽  
Benhe Gong ◽  
Xing Cao ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transcription Factor ◽  
Anther Development ◽  
Lilium Oriental Hybrids

scholarly journals Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ruimin Zhang ◽  
Jingjing Chang ◽  
Jiayue Li ◽  
Guangpu Lan ◽  
Changqing Xuan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Male Sterility ◽  
Molecular Mechanisms ◽  
Citrullus Lanatus ◽  
Anther Development ◽  
Dose Effect ◽  
Luciferase Reporter ◽  
Bhlh Transcription Factor ◽  
Transcriptional Level ◽  
Hybrid Seed Production

AbstractAlthough male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production, its underlying molecular mechanisms in Cucurbitaceae species are still largely unclear. Here, a spontaneous male-sterile watermelon mutant, Se18, was reported to have abnormal tapetum development, which resulted in completely aborted pollen grains. Map-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1 (ClATM1) encodes a basic helix-loop-helix (bHLH) transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional (BIF) domain in Se18 plants. qRT–PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6–8a of anther development. The genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated mutagenesis. Moreover, ClATM1 was significantly downregulated in the Se18 mutant, displaying a clear dose effect at the transcriptional level. Subsequent dual-luciferase reporter, β-glucuronidase (GUS) activity, and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter binding. Collectively, ClATM1 is the first male sterility gene cloned from watermelon, and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants.

scholarly journals A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

2021 ◽  
Author(s):  
Junping Yu ◽  
Guolong Zhao ◽  
Wei Li ◽  
Ying Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Male Sterility ◽  
Bulked Segregant Analysis ◽  
Soybean Protein ◽  
Anther Development ◽  
Hybrid Breeding ◽  
Myb Transcription Factor ◽  
Male Sterile ◽  
R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

scholarly journals Conserved and non-conserved functions of the rice homologs of the Arabidopsis trichome initiation-regulating MBW complex proteins

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Kaijie Zheng ◽  
Xutong Wang ◽  
Yating Wang ◽  
Shucai Wang
Keyword(s):  
Transcription Factor ◽  
Anthocyanin Biosynthesis ◽  
Transcriptional Activator ◽  
Myb Transcription Factor ◽  
Bhlh Transcription Factor ◽  
Seed Color ◽  
Transcription Activator ◽  
Root Hair Formation ◽  
Protoplast Transfection ◽  
Mbw Complex

Abstract Background Trichome initiation in Arabidopsis is regulated by a MYB-bHLH-WD40 (MBW) transcriptional activator complex formed by the R2R3 MYB transcription factor GLABRA1 (GL1), MYB23 or MYB82, the bHLH transcription factor GLABRA3 (GL3), ENHANCER OF GLABRA3 (EGL3) or TRANSPARENT TESTA8 (TT8), and the WD40-repeat protein TRANSPARENT TESTA GLABRA1 (TTG1). However, the functions of the rice homologs of the MBW complex proteins remained uncharacterized. Results Based on amino acid sequence identity and similarity, and protein interaction prediction, we identified OsGL1s, OsGL3s and OsTTG1s as rice homologs of the MBW complex proteins. By using protoplast transfection, we show that OsGL1D, OsGL1E, OsGL3B and OsTTG1A were predominantly localized in the nucleus, OsGL3B functions as a transcriptional activator and is able to interact with GL1 and TTG1. By using yeast two-hybrid and protoplast transfection assays, we show that OsGL3B is able to interact with OsGL1E and OsTTG1A, and OsGL1E and OsTTG1A are also able to interact with GL3. On the other hand, we found that OsGL1D functions as a transcription activator, and it can interact with GL3 but not OsGL3B. Furthermore, our results show that expression of OsTTG1A in the ttg1 mutant restored the phenotypes including alternations in trichome and root hair formation, seed color, mucilage production and anthocyanin biosynthesis, indicating that OsTTG1A and TTG1 may have similar functions. Conclusion These results suggest that the rice homologs of the Arabidopsis MBW complex proteins are able to form MBW complexes, but may have conserved and non-conserved functions.

scholarly journals The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples

2012 ◽  
Vol 35 (11) ◽  
pp. 1884-1897 ◽  
Author(s):  
XING-BIN XIE ◽  
SHEN LI ◽  
RUI-FEN ZHANG ◽  
JING ZHAO ◽  
YING-CHUN CHEN ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Low Temperature ◽  
Bhlh Transcription Factor ◽  
Anthocyanin Accumulation ◽  
Fruit Colouration

scholarly journals An Atypical bHLH Transcription Factor Regulates Early Xylem Development Downstream of Auxin

2013 ◽  
Vol 54 (3) ◽  
pp. 398-405 ◽  
Author(s):  
Kyoko Ohashi-Ito ◽  
Manami Matsukawa ◽  
Hiroo Fukuda
Keyword(s):  
Transcription Factor ◽  
Bhlh Transcription Factor ◽  
Xylem Development

scholarly journals Involvement of c-Myc in the proliferation of MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2

2014 ◽  
Vol 35 (3) ◽  
pp. 815-820 ◽  
Author(s):  
YUNYAN WU ◽  
HIDENOBU SATO ◽  
TAKAHIRO SUZUKI ◽  
TADASHI YOSHIZAWA ◽  
SATOKO MOROHASHI ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Bhlh Transcription Factor ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7
Sign in / Sign up

Export Citation Format

Share Document