The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples

Chilling injury poses a serious threat to seed emergence of spring-sowing maize in China, which has become one of the main climatic limiting factors affecting maize production in China. It is of great significance to mine the key genes controlling low-temperature tolerance during seed germination and study their functions for breeding new maize varieties with strong low-temperature tolerance during germination. In this study, 176 lines of the intermated B73 × Mo17 (IBM) Syn10 doubled haploid (DH) population, which comprised 6618 bin markers, were used for QTL analysis of low-temperature germination ability. The results showed significant differences in germination related traits under optimum-temperature condition (25 °C) and low-temperature condition (10 °C) between two parental lines. In total, 13 QTLs were detected on all chromosomes, except for chromosome 5, 7, 10. Among them, seven QTLs formed five QTL clusters on chromosomes 1, 2, 3, 4, and 9 under the low-temperature condition, which suggested that there may be some genes regulating multiple germination traits at the same time. A total of 39 candidate genes were extracted from five QTL clusters based on the maize GDB under the low-temperature condition. To further screen candidate genes controlling low-temperature germination, RNA-Seq, in which RNA was extracted from the germination seeds of B73 and Mo17 at 10 °C, was conducted, and three B73 upregulated genes and five Mo17 upregulated genes were found by combined analysis of RNA-Seq and QTL located genes. Additionally, the variations of Zm00001d027976 (GLABRA2), Zm00001d007311 (bHLH transcription factor), and Zm00001d053703 (bZIP transcription factor) were found by comparison of amino sequence between B73 and Mo17. This study will provide a theoretical basis for marker-assisted breeding and lay a foundation for further revealing molecular mechanism of low-temperature germination tolerance in maize.

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FvbHLH9 Functions as a Positive Regulator of Anthocyanin Biosynthesis by Forming a HY5–bHLH9 Transcription Complex in Strawberry Fruits

Plant and Cell Physiology ◽

10.1093/pcp/pcaa010 ◽

2020 ◽

Vol 61 (4) ◽

pp. 826-837 ◽

Cited By ~ 4

Author(s):

Yang Li ◽

Pengbo Xu ◽

Guanqun Chen ◽

Jun Wu ◽

Zhongchi Liu ◽

...

Keyword(s):

Transcription Factor ◽

Promoter Region ◽

Anthocyanin Biosynthesis ◽

Anthocyanin Synthesis ◽

Bhlh Transcription Factor ◽

Anthocyanin Accumulation ◽

Positive Regulator ◽

Key Enzyme ◽

Synergistic Regulation ◽

Transient Overexpression

Abstract Anthocyanin accumulation is transcriptionally regulated by the MYB–bHLH–WD40 complex. Light is indispensable for anthocyanin accumulation, and light-inducible MYB and HY5 were considered to promote anthocyanin accumulation in many fruits. Whether and how light-inducible bHLH transcription factor and HY5 regulate anthocyanin synthesis in strawberry is unknown. In this study, we identified a bHLH transcription factor, FvbHLH9, which was induced by light as well as FvHY5, and found that, similar to FvHY5, the transient overexpression and interference FvbHLH9 in strawberry fruits can promote and decrease anthocyanin accumulation, respectively, indicating FvbHLH9 functions as a positive regulator of anthocyanin biosynthesis. Furthermore, we confirmed that both FvHY5 and FvbHLH9 specifically bind to the promoter region of some key enzyme genes, including FvDFR, and the expression of FvDFR was activated through the heterodimer formation between FvHY5 and FvbHLH9. Finally, we confirmed that FvbHLH9-promoted anthocyanin accumulation is dependent on HY5–bHLH heterodimerisation in Arabidopsis. Our findings provide insights into a mechanism involving the synergistic regulation of light-dependent coloration and anthocyanin biosynthesis via a HY5–bHLH heterodimer formed by the interaction of FvHY5 and FvbHLH9 in strawberry fruits.

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Expression of the subgroup IIIf bHLH transcription factor CpbHLH1 from Chimonanthus praecox (L.) in transgenic model plants inhibits anthocyanin accumulation

Plant Cell Reports ◽

10.1007/s00299-020-02537-9 ◽

2020 ◽

Vol 39 (7) ◽

pp. 891-907

Author(s):

Rong Zhao ◽

Xiaoxi Song ◽

Nan Yang ◽

Longqing Chen ◽

Lin Xiang ◽

...

Keyword(s):

Transcription Factor ◽

Bhlh Transcription Factor ◽

Anthocyanin Accumulation ◽

Transgenic Model ◽

Chimonanthus Praecox

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Faculty Opinions recommendation of bHLH transcription factor Olig1 is required to repair demyelinated lesions in the CNS.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1023116.264810 ◽

2005 ◽

Author(s):

Magdalena Goetz

Keyword(s):

Transcription Factor ◽

Bhlh Transcription Factor

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Faculty Opinions recommendation of Mutation of a bHLH transcription factor allowed almond domestication.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.735980063.793561372 ◽

2019 ◽

Author(s):

François Parcy

Keyword(s):

Transcription Factor ◽

Bhlh Transcription Factor

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Postharvest temperature and light treatments induce anthocyanin accumulation in peel of ‘Akihime’ plum (Prunus salicina Lindl.) via transcription factor PsMYB10.1

Postharvest Biology and Technology ◽

10.1016/j.postharvbio.2021.111592 ◽

2021 ◽

Vol 179 ◽

pp. 111592

Author(s):

Zhizhen Fang ◽

Kui Lin-Wang ◽

Cuicui Jiang ◽

Danrong Zhou ◽

Yanjuan Lin ◽

...

Keyword(s):

Transcription Factor ◽

Anthocyanin Accumulation ◽

Prunus Salicina

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Conserved and non-conserved functions of the rice homologs of the Arabidopsis trichome initiation-regulating MBW complex proteins

BMC Plant Biology ◽

10.1186/s12870-021-03035-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Kaijie Zheng ◽

Xutong Wang ◽

Yating Wang ◽

Shucai Wang

Keyword(s):

Transcription Factor ◽

Anthocyanin Biosynthesis ◽

Transcriptional Activator ◽

Myb Transcription Factor ◽

Bhlh Transcription Factor ◽

Seed Color ◽

Transcription Activator ◽

Root Hair Formation ◽

Protoplast Transfection ◽

Mbw Complex

Abstract Background Trichome initiation in Arabidopsis is regulated by a MYB-bHLH-WD40 (MBW) transcriptional activator complex formed by the R2R3 MYB transcription factor GLABRA1 (GL1), MYB23 or MYB82, the bHLH transcription factor GLABRA3 (GL3), ENHANCER OF GLABRA3 (EGL3) or TRANSPARENT TESTA8 (TT8), and the WD40-repeat protein TRANSPARENT TESTA GLABRA1 (TTG1). However, the functions of the rice homologs of the MBW complex proteins remained uncharacterized. Results Based on amino acid sequence identity and similarity, and protein interaction prediction, we identified OsGL1s, OsGL3s and OsTTG1s as rice homologs of the MBW complex proteins. By using protoplast transfection, we show that OsGL1D, OsGL1E, OsGL3B and OsTTG1A were predominantly localized in the nucleus, OsGL3B functions as a transcriptional activator and is able to interact with GL1 and TTG1. By using yeast two-hybrid and protoplast transfection assays, we show that OsGL3B is able to interact with OsGL1E and OsTTG1A, and OsGL1E and OsTTG1A are also able to interact with GL3. On the other hand, we found that OsGL1D functions as a transcription activator, and it can interact with GL3 but not OsGL3B. Furthermore, our results show that expression of OsTTG1A in the ttg1 mutant restored the phenotypes including alternations in trichome and root hair formation, seed color, mucilage production and anthocyanin biosynthesis, indicating that OsTTG1A and TTG1 may have similar functions. Conclusion These results suggest that the rice homologs of the Arabidopsis MBW complex proteins are able to form MBW complexes, but may have conserved and non-conserved functions.

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