Construction and in vivo evaluation of a mammary gland-specific expression vector for human lysozyme

Plasmid ◽  
2014 ◽  
Vol 76 ◽  
pp. 47-53 ◽  
Author(s):  
Guocai Li ◽  
Weiqing Shi ◽  
Gang Chen ◽  
Hongju Chen ◽  
Hongmei Jiao ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Expression Vector ◽  
Human Lysozyme ◽  
In Vivo Evaluation ◽  
Specific Expression

scholarly journals Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhipeng Zhang ◽  
Daijie Chen ◽  
Xubin Lu ◽  
Ruifeng Zhao ◽  
Zhi Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Antimicrobial Peptide ◽  
Expression Vector ◽  
Bovine Mastitis ◽  
Specific Expression ◽  
Mammary Gland Tissue ◽  
Gland Tissue

Bovine mastitis is perplexing the dairy industry since the initiation of intensive dairy farming, which has caused a reduction in the productivity of cows and an escalation in costs. The use of antibiotics causes a series of problems, especially the formation of bacterial antimicrobial resistance. However, there are limited antibiotic-free therapeutic strategies that can effectively relieve bacterial infection of bovine mammary glands. Hence, in this study, we constructed a mammary gland tissue-specific expression vector carrying the antimicrobial peptide of bovine-derived tracheal antimicrobial peptide (TAP) and evaluated it in both primary bovine mammary epithelial cells (pBMECs) and mice. The results showed that the vector driven by the β-lactoglobulin gene (BLG) promoter could efficiently direct the expression of TAP in pBMECs and the mammary gland tissue of mice. In addition, significant antibacterial effects were observed in both in vitro and in vivo experiments when introducing this vector to bovine-associated Staphylococcus aureus-treated pBMECs and mice, respectively. This study demonstrated that the mammary gland tissue-specific expression vector could be used to introduce antimicrobial peptide both in in vitro and in vivo and will provide a new therapeutic strategy in the treatment of bovine mastitis.

Expression and characterization of a lysine rich protein in cow milk

2018 ◽  
Author(s):  
Xin Ma ◽  
Li Su ◽  
Peng Zhang ◽  
Sheng Zhang ◽  
Bo Tang ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Amino Acid ◽  
Expression Vector ◽  
Cow Milk ◽  
Specific Expression ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Negative Controls ◽  
Mammary Gland Expression

The lysine is considered as the most important essential amino acid, because it is the most limiting in the cereals grains. In this study, a lysine-rich (LR) gene, and the expression vector pcDNA3.1-LR and pBC1-LR were constructed. The LR was expressed in 293T cells driven by the vector pcDNA3.1-LR and checked by RT-PCR and WB. The mammary gland tissue-specific expression vector carrying the LR was injected directly into the lactating mammary glands of cows and the milk samples were checked by a complete amino acid analysis. The results showed that the LR protein was expressed successfully in cells and in cow milk; the expression of LR lasted for 6 d, and the lysine level of the injection group was significantly higher than that of negative controls (p Lass Than 0.05). This study provide a better understanding of how mammary gland expression systems increase the lysine content of milk that can be applied to transgenic dairy cow.

scholarly journals Development of a heavy metal-inducible fish-specific expression vector for gene transfer in vitro and in vivo

Aquaculture ◽  
1993 ◽  
Vol 111 (1-4) ◽  
pp. 215-226 ◽  
Author(s):  
Yunhan Hong ◽  
Christoph Winkler ◽  
Gottfried Brem ◽  
Manfred Schartl
Keyword(s):  
Heavy Metal ◽  
Gene Transfer ◽  
Expression Vector ◽  
Specific Expression

scholarly journals In vivo evaluation of GG2–GG1/A2 element activity in the insulin promoter region using the CRISPR–Cas9 system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hirofumi Noguchi ◽  
Chika Miyagi-Shiohira ◽  
Takao Kinjo ◽  
Issei Saitoh ◽  
Masami Watanabe
Keyword(s):  
Promoter Region ◽  
In Vivo Studies ◽  
Pancreatic Β Cell ◽  
Β Cell ◽  
In Vivo Evaluation ◽  
Specific Expression ◽  
Insulin Promoter ◽  
Element Activity

AbstractThe insulin promoter is regulated by ubiquitous as well as pancreatic β-cell-specific transcription factors. In the insulin promoter, GG2–GG1/A2–C1 (bases − 149 to − 116 in the human insulin promoter) play important roles in regulating β-cell-specific expression of the insulin gene. However, these events were identified through in vitro studies, and we are unaware of comparable in vivo studies. In this study, we evaluated the activity of GG2–GG1/A2 elements in the insulin promoter region in vivo. We generated homozygous mice with mutations in the GG2–GG1/A2 elements in each of the Ins1 and Ins2 promoters by CRISPR–Cas9 technology. The mice with homozygous mutations in the GG2–GG1/A2 elements in both Ins1 and Ins2 were diabetic. These data suggest that the GG2–GG1/A2 element in mice is important for Ins transcription in vivo.

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