Expression and characterization of a lysine rich protein in cow milk

2018 ◽  
Author(s):  
Xin Ma ◽  
Li Su ◽  
Peng Zhang ◽  
Sheng Zhang ◽  
Bo Tang ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Amino Acid ◽  
Expression Vector ◽  
Cow Milk ◽  
Specific Expression ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Negative Controls ◽  
Mammary Gland Expression

The lysine is considered as the most important essential amino acid, because it is the most limiting in the cereals grains. In this study, a lysine-rich (LR) gene, and the expression vector pcDNA3.1-LR and pBC1-LR were constructed. The LR was expressed in 293T cells driven by the vector pcDNA3.1-LR and checked by RT-PCR and WB. The mammary gland tissue-specific expression vector carrying the LR was injected directly into the lactating mammary glands of cows and the milk samples were checked by a complete amino acid analysis. The results showed that the LR protein was expressed successfully in cells and in cow milk; the expression of LR lasted for 6 d, and the lysine level of the injection group was significantly higher than that of negative controls (p Lass Than 0.05). This study provide a better understanding of how mammary gland expression systems increase the lysine content of milk that can be applied to transgenic dairy cow.

scholarly journals Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhipeng Zhang ◽  
Daijie Chen ◽  
Xubin Lu ◽  
Ruifeng Zhao ◽  
Zhi Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Antimicrobial Peptide ◽  
Expression Vector ◽  
Bovine Mastitis ◽  
Specific Expression ◽  
Mammary Gland Tissue ◽  
Gland Tissue

Bovine mastitis is perplexing the dairy industry since the initiation of intensive dairy farming, which has caused a reduction in the productivity of cows and an escalation in costs. The use of antibiotics causes a series of problems, especially the formation of bacterial antimicrobial resistance. However, there are limited antibiotic-free therapeutic strategies that can effectively relieve bacterial infection of bovine mammary glands. Hence, in this study, we constructed a mammary gland tissue-specific expression vector carrying the antimicrobial peptide of bovine-derived tracheal antimicrobial peptide (TAP) and evaluated it in both primary bovine mammary epithelial cells (pBMECs) and mice. The results showed that the vector driven by the β-lactoglobulin gene (BLG) promoter could efficiently direct the expression of TAP in pBMECs and the mammary gland tissue of mice. In addition, significant antibacterial effects were observed in both in vitro and in vivo experiments when introducing this vector to bovine-associated Staphylococcus aureus-treated pBMECs and mice, respectively. This study demonstrated that the mammary gland tissue-specific expression vector could be used to introduce antimicrobial peptide both in in vitro and in vivo and will provide a new therapeutic strategy in the treatment of bovine mastitis.

Spectral Characterization of Fluorescently Labeled Catechol Estrogen 3,4-Quinone-Derived N7 Guanine Adducts and Their Identification in Rat Mammary Gland Tissue

1998 ◽  
Vol 11 (11) ◽  
pp. 1339-1345 ◽  
Author(s):  
Ryszard Jankowiak ◽  
Dan Zamzow ◽  
Douglas E. Stack ◽  
Rosa Todorovic ◽  
Ercole L. Cavalieri ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Spectral Characterization ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Guanine Adducts ◽  
Catechol Estrogen ◽  
Rat Mammary Gland

scholarly journals Characterization of microRNA profiles in the mammary gland tissue of dairy goats at the late lactation, dry period and late gestation stages

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0234427
Author(s):  
Rong Xuan ◽  
Tianle Chao ◽  
Aili Wang ◽  
Fuhong Zhang ◽  
Ping Sun ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Late Gestation ◽  
Dairy Goats ◽  
Dry Period ◽  
Mammary Gland Tissue ◽  
Gland Tissue

scholarly journals CHARACTERIZATION OF MAMMARY GLAND TISSUE USING JOINT ESTIMATORS OF MINKOWSKI FUNCTIONALS

2011 ◽  
Vol 26 (1) ◽  
pp. 13 ◽  
Author(s):  
Torsten Mattfeldt ◽  
Daniel Meschenmoser ◽  
Ursa Pantle ◽  
Volker Schmidt
Keyword(s):  
Mammary Gland ◽  
Image Data ◽  
Estimation Procedure ◽  
Asymptotic Covariance Matrix ◽  
Real Image ◽  
Minkowski Functionals ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Least Squares Approach

A theoretical approach to estimate the Minkowski functionals, i.e., area fraction, specifc boundary length and specifc Euler number in 2D, and their asymptotic covariance matrix proposed by Spodarev and Schmidt (2005) and Pantle et al. (2006a;b) is applied to real image data. These two-dimensional images show mammary gland tissue and should be classifed automatically as tumor-free or mammary cancer, respectively. The estimation procedure is illustrated step-by-step and the calculations are described in detail. To reduce dependencies from chosen parameters, a least-squares approach is considered as recommended by Klenk et al. (2006). Emphasis is placed on the detailed description of the estimation procedure and the application of the theory to real image data.

PROTEOME OF MILK FAT GLOBULE MEMBRANE AND MAMMARY GLAND TISSUE IN GOAT FED DIFFERENT LIPID SUPPLEMENTATION

2021 ◽  
pp. 106378
Author(s):  
Iolly Tábata Oliveira Marques ◽  
Fábio Roger Vasconcelos ◽  
Juliana Paula Martins Alves ◽  
Assis Rubens Montenegro ◽  
César Carneiro Linhares Fernandes ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Milk Fat ◽  
Milk Fat Globule Membrane ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Milk Fat Globule ◽  
Lipid Supplementation ◽  
Fat Globule

Cloning and characterization of sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) from crayfish axial muscle. Sarco/Endoplasmic Reticulum Ca(2+)-ATPase

2000 ◽  
Vol 203 (22) ◽  
pp. 3411-3423 ◽  
Author(s):  
Z. Zhang ◽  
D. Chen ◽  
M.G. Wheatly
Keyword(s):  
Endoplasmic Reticulum ◽  
Amino Acid ◽  
Procambarus Clarkii ◽  
Muscle Growth ◽  
Abdominal Muscle ◽  
Northern Analysis ◽  
Specific Expression ◽  
Loop Region ◽  
Moulting Cycle

The discontinuous pattern of muscle growth during the moulting cycle of a freshwater crustacean (the crayfish Procambarus clarkii) was used as a model system to examine the regulation of the expression of Sarco/Endoplasmic Reticulum Ca(2+)-ATPase (SERCA). We describe the cloning, sequencing and characterization of a novel SERCA cDNA (3856 bp) obtained from crayfish axial abdominal muscle by reverse transcription/polymerase chain reaction (RT-PCR) followed by rapid amplification of cDNA ends (RACE). This complete sequence contains a 145 base pair (bp) noncoding region at the 5′ end, a 3006 bp open reading frame coding for 1002 amino acid residues with a molecular mass of 110 kDa and 705 bp of untranslated region at the 3′ end. This enzyme contains all the conserved domains found in ‘P’-type ATPases, and the hydropathy profile suggests a transmembrane organization typical of other SERCAs. It exhibits 80% amino acid identity with Drosophila melanogaster SERCA, 79% identity with Artemia franciscana SERCA, 72% identity with rabbit fast-twitch muscle neonatal isoform SERCA1b, 71% identity with slow-twitch muscle isoform SERCA2 and 67% identity with SERCA3. Sequence alignment revealed that regions anchoring the cytoplasmic domain in the membrane were highly conserved and that most differences were in the NH(2) terminus, the central loop region and the COOH terminus. Northern analysis of total RNA from crayfish tissues probed with the 460 bp fragment initially isolated showed four bands (7.6, 7.0, 5.8 and 4.5 kilobases) displaying tissue-specific expression. SERCA was most abundant in muscle (axial abdominal, cardiac and stomach), where it is involved in Ca(2+) resequestration during relaxation, and in eggs, where it may be implicated in early embryogenesis. The level of SERCA mRNA expression in axial abdominal muscle varied during the moulting cycle as determined by slot-blot analysis. SERCA expression was greatest during intermoult and decreased to approximately 50% of this level during pre- and postmoult. Patterns of gene expression for SERCA and other sarcomeric proteins during the crustacean moulting cycle may be regulated by ecdysteroids and/or mechanical stimulation.

Sign in / Sign up

Export Citation Format

Share Document