Multi-target plasmid controls for conventional and real-time PCR-based serotyping of Streptococcus pneumoniae

Plasmid ◽  
2018 ◽  
Vol 98 ◽  
pp. 45-51 ◽  
Author(s):  
Jack Schembri ◽  
Hayley D. Gillis ◽  
Amanda L.S. Lang ◽  
Michelle Warhuus ◽  
Irene Martin ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Target Plasmid

Detection of Streptococcus pneumoniae in Sputum Samples by Real- Time PCR.

2020 ◽  
Vol 18 ◽  
Author(s):  
Pegah Shakib ◽  
Mohammad Reza Zolfaghari
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
False Negative ◽  
Cross Sectional Study ◽  
Laboratory Culture ◽  
Cross Sectional ◽  
Negative Results ◽  
False Negative Results ◽  
Pcr Method

Background: Conventional laboratory culture-based methods for diagnosis of Streptococcus pneumoniae are time-consuming and yield false negative results. Molecular methods including real-time (RT)-PCR rapid methods and conventional PCR due to higher sensitivity and accuracy have been replaced instead traditional culture assay. The aim of the current study was to evaluate lytA gene for detection of Streptococcus pneumoniae in the cerebrospinal fluid of human patients with meningitis using real-time PCR assay. Material and Methods: In this cross-sectional study, a total of 30 clinical specimens were collected from patients in a period from September to December 2018. In order to evaluate the presence of lytA gene, conventional and real-time PCR methods were used without culture. Results: From 30 sputum samples five (16.66%) isolates were identified as S. pneumoniae by lytA PCR and sequencing. Discussion: In this research, an accurate and rapid real-time PCR method was used, which is based on lytA gene for diagnosis of bacteria so that it can be diagnosed. Based on the sequencing results, the sensitivity for detection of lytA gene was 100% (5/5).

Identification of Streptococcus pneumoniae lytA , plyA and psaA genes in pleural fluid by multiplex real-time PCR

2018 ◽  
Vol 36 (7) ◽  
pp. 428-430 ◽  
Author(s):  
Juan Carlos Sanz ◽  
Esther Ríos ◽  
Iciar Rodríguez-Avial ◽  
Belén Ramos ◽  
Mercedes Marín ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Pleural Fluid ◽  
Real Time Pcr

scholarly journals Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR

2011 ◽  
Vol 10 (60) ◽  
pp. 12826-12832 ◽  
Author(s):  
Nomanpour Bizhan ◽  
Ghodousi Arash ◽  
Babaei Toraj ◽  
AliJavad Mousavi Seyd ◽  
Asadi Soroor ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Iranian Patients ◽  
Detection And Quantification

scholarly journals Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Henry M Wu ◽  
Soraia M Cordeiro ◽  
Brian H Harcourt ◽  
Maria da Gloria S Carvalho ◽  
Jailton Azevedo ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Real Time ◽  
Real Time Pcr ◽  
Gram Stain

Evaluation of lytB Gene for Detection of Streptococcus pneumoniae in Isolates and Clinical Specimens by Real-Time PCR

2017 ◽  
Vol 10 (6) ◽  
Author(s):  
Mohammad Azarsa ◽  
Seyed Alireza Salami ◽  
Mohammad Reza Pourmand ◽  
Abbas Rahimi Forushani ◽  
Hamid Kazemian
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Specimens

Detection of parC mutations in Streptococcus pneumoniae by Real-time PCR and Taqman–MGB probes

2007 ◽  
Vol 69 (1) ◽  
pp. 214-217 ◽  
Author(s):  
Rodrigo Alonso ◽  
Estibaliz Mateo ◽  
Ramón Cisterna
Keyword(s):  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Real Time Pcr
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