Gene expression profiling of virulence-associated proteins in planta during net blotch disease of barley

Bioluminescence is a powerful biological signal that scientists have repurposed as a reporter for gene expression in plants and animals. However, there are downsides associated with the need to provide a substrate to these reporters, including its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using a composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for future construction of programmable auto-luminescent plant traits, such as light driven plant-pollinator interactions or light emitting plant-based sensors.

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Building customizable auto-luminescent luciferase-based reporters in plants

10.1101/809533 ◽

2019 ◽

Cited By ~ 3

Author(s):

Arjun Khakhar ◽

Colby Starker ◽

James Chamness ◽

Nayoung Lee ◽

Sydney Stokke ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Expression Profiling ◽

Plant Traits ◽

Low Cost ◽

In Planta ◽

Tissue Penetration ◽

Light Emitting ◽

Plant Pollinator ◽

Substrate Addition

AbstractBioluminescence is a powerful biological signal that scientists have repurposed to design reporters for gene expression in plants and animals. However, there are some downsides associated with the need to provide a substrate to these reporters, such as its high cost and non-uniform tissue penetration. In this work we reconstitute a fungal bioluminescence pathway (FBP) in planta using an easily composable toolbox of parts. We demonstrate that the FBP can create luminescence across various tissues in a broad range of plants without external substrate addition. We also show how our toolbox can be used to deploy the FBP in planta to build auto-luminescent reporters for the study of gene-expression and hormone fluxes. A low-cost imaging platform for gene expression profiling is also described. These experiments lay the groundwork for the future construction of programmable auto-luminescent plant traits, such as creating light driven plant-pollinator interactions or light emitting plant-based sensors.

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Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling

Biochemical Society Symposium ◽

10.1042/bss0690135 ◽

2002 ◽

Vol 69 ◽

pp. 135-142 ◽

Cited By ~ 12

Author(s):

Elena M. Comelli ◽

Margarida Amado ◽

Steven R. Head ◽

James C. Paulson

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Expression Profiling ◽

Affymetrix Genechip ◽

Microarray Technology ◽

Gene Arrays ◽

Glycosyltransferase Gene

The development of microarray technology offers the unprecedented possibility of studying the expression of thousands of genes in one experiment. Its exploitation in the glycobiology field will eventually allow the parallel investigation of the expression of many glycosyltransferases, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis. While numerous gene arrays are available on the market, e.g. the Affymetrix GeneChip® arrays, glycosyltransferases are not adequately represented, which makes comprehensive surveys of their gene expression difficult. This chapter describes the main issues related to the establishment of a custom glycogenes array.

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