Differentiation of Cherry leaf roll virus isolates from walnut based on molecular analyses and reaction of herbaceous hosts

2020 ◽  
Vol 109 ◽  
pp. 101456
Author(s):  
Mirosława Cieślińska
Keyword(s):  
Leaf Roll ◽  
Molecular Analyses ◽  
Cherry Leaf Roll Virus ◽  
Virus Isolates

scholarly journals Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 574
Author(s):  
Evanthia Xylogianni ◽  
Paolo Margaria ◽  
Dennis Knierim ◽  
Kyriaki Sareli ◽  
Stephan Winter ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Leaf Roll ◽  
Virus Infections ◽  
Northern Greece ◽  
Coat Protein Region ◽  
Cherry Leaf Roll Virus ◽  
Leaf Yellowing ◽  
Olive Trees ◽  
Olive Varieties ◽  
Viral Sequences

Field surveys were conducted in Greek olive orchards from 2017 to 2020 to collect information on the sanitary status of the trees. Using a high-throughput sequencing approach, viral sequences were identified in total RNA extracts from several trees and assembled to reconstruct the complete genomes of two isolates of a new viral species of the genus Tepovirus (Betaflexiviridae), for which the name olive virus T (OlVT) is proposed. A reverse transcription–polymerase chain reaction assay was developed which detected OlVT in samples collected in olive growing regions in Central and Northern Greece, showing a virus prevalence of 4.4% in the olive trees screened. Sequences of amplified fragments from the movement–coat protein region of OlVT isolates varied from 75.64% to 99.35%. Three olive varieties (Koroneiki, Arbequina and Frantoio) were infected with OlVT via grafting to confirm a graft-transmissible agent, but virus infections remained latent. In addition, cucumber mosaic virus, olive leaf yellowing-associated virus and cherry leaf roll virus were identified.

scholarly journals Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RT-PCR

2009 ◽  
Vol 45 (No. 4) ◽  
pp. 140-143 ◽  
Author(s):  
S. Kumari
Keyword(s):  
Leaf Roll ◽  
Rt Pcr ◽  
Spot Virus ◽  
Cherry Leaf Roll Virus ◽  
One Step ◽  
Polymerase Chain ◽  
The One ◽  
Ring Spot ◽  
Screen House ◽  
Xiphinema Diversicaudatum

A one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of <i>Cherry leaf roll virus</i> (CLRV) and <i>Strawberry latent ring spot virus</i> (SLRSV). The protocol was used to test infected screen house plants and also plants from orchards and vineyards where the vector (<i>Xiphinema diversicaudatum</i>) of SLRSV was detected from the soil. The one-step RT-PCR protocol is rapid and sensitive and has the potential to be used for the diagnosis of CLRV and SLRSV in routine diagnostic laboratories.

Serological Comparison of Isolates of Cherry Leaf Roll Virus from Diseased Beech and Birch Trees in a Forest Decline Area in Germany with Other Isolates of the Virus

1990 ◽  
Vol 129 (4) ◽  
pp. 339-344 ◽  
Author(s):  
A. T. Jones ◽  
Renate Koenig ◽  
D.-E. Lesemann ◽  
J. Hamacher ◽  
F. Nienhaus ◽  
...  
Keyword(s):  
Forest Decline ◽  
Leaf Roll ◽  
Cherry Leaf Roll Virus ◽  
Birch Trees

Complete nucleotide sequence of Cherry leaf roll virus (CLRV), a subgroup C nepovirus

2012 ◽  
Vol 163 (2) ◽  
pp. 678-683 ◽  
Author(s):  
Susanne von Bargen ◽  
Juliane Langer ◽  
Jenny Robel ◽  
Artemis Rumbou ◽  
Carmen Büttner
Keyword(s):  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Leaf Roll ◽  
Cherry Leaf Roll Virus

Tests for transmission of cherry leaf roll virus using Longidorus, Paralongidorus and Xiphinema nematodes

1981 ◽  
Vol 99 (2) ◽  
pp. 143-150 ◽  
Author(s):  
A. T. JONES ◽  
F. D. McELROY ◽  
D. J. F. BROWN
Keyword(s):  
Leaf Roll ◽  
Cherry Leaf Roll Virus
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