Ethylene biosynthesis is involved in regulating chilling tolerance and SlCBF1 gene expression in tomato fruit

In a 2-year study (1993-1994), `New Yorker' tomato (Lycopersicon esculentum Mill.) plants grown in field lysimeters were subjected to four watertable depth (WTD) treatments (0.3, 0.6, 0.8, and 1.0 m from the soil surface) factorially combined with 5 potassium/calcium fertilization combinations. Mature-green fruit from four replicates of each treatment were stored at 5C for 21 days, and fruit color was monitored with a tristimulus colorimeter. Fruit were subsequently allowed to ripen at 20C for 10 days, at which time chilling injury was assessed on the basis of delayed ripening and area of lesions. Potassium and calcium applied in the field had no effect on chilling tolerance of the fruit. In the drier year (1993), shallower WTD treatments generally yielded fruit that changed color less during chilling and were more chilling-sensitive based on delayed ripening. In the wetter year, differences in color change and chilling tolerance between WTD, if any, were small. Over both years, lesion area varied with WTD, but not in a consistent manner. Based on these results, we suggest that differences in water availability should be considered when studying tomato fruit chilling.

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SlMYC2 Involved in Methyl Jasmonate-Induced Tomato Fruit Chilling Tolerance

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.8b00299 ◽

2018 ◽

Vol 66 (12) ◽

pp. 3110-3117 ◽

Cited By ~ 16

Author(s):

Dedong Min ◽

Fujun Li ◽

Xinhua Zhang ◽

Xixi Cui ◽

Pan Shu ◽

...

Keyword(s):

Methyl Jasmonate ◽

Tomato Fruit ◽

Chilling Tolerance

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NAC transcription factors play an important role in ethylene biosynthesis, reception and signaling of tomato fruit ripening

Molecular Genetics and Genomics ◽

10.1007/s00438-016-1177-0 ◽

2016 ◽

Vol 291 (3) ◽

pp. 1205-1217 ◽

Cited By ~ 30

Author(s):

Xiaohong Kou ◽

Chen Liu ◽

Lihua Han ◽

Shuang Wang ◽

Zhaohui Xue

Keyword(s):

Transcription Factors ◽

Fruit Ripening ◽

Tomato Fruit ◽

Ethylene Biosynthesis ◽

Nac Transcription Factors ◽

Tomato Fruit Ripening

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Induction and Repression of Gene Expression Mediating Ethylene Biosynthesis and Sodium/Proton Exchange in Rice Seedlings Under Submergence Stress

Biotechnology & Biotechnological Equipment ◽

10.5504/bbeq.2012.0043 ◽

2012 ◽

Vol 26 (3) ◽

pp. 2945-2951

Author(s):

Hien Thi Thu Vu ◽

Oliver Escano Manangkil ◽

Naoki Mori ◽

Shinya Yoshida ◽

Chiharu Nakamura

Keyword(s):

Gene Expression ◽

Ethylene Biosynthesis ◽

Proton Exchange ◽

Rice Seedlings ◽

Submergence Stress ◽

Sodium Proton Exchange

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Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

Journal of Experimental Botany ◽

10.1093/jxb/eraa131 ◽

2020 ◽

Vol 71 (12) ◽

pp. 3560-3574 ◽

Cited By ~ 7

Author(s):

Ying Gao ◽

Wei Wei ◽

Zhongqi Fan ◽

Xiaodan Zhao ◽

Yiping Zhang ◽

...

Keyword(s):

Fruit Ripening ◽

Tomato Fruit ◽

Pectate Lyase ◽

Ethylene Biosynthesis ◽

Fruit Softening ◽

Wild Type ◽

Gain Of Function ◽

Carotenoid Accumulation ◽

Acid Protein ◽

Tomato Fruit Ripening

Abstract The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be a gain-of-function mutant. Here, we provide more evidence to support this view and answer the open question of whether the NAC-NOR gene is important in fruit ripening. Overexpression of NAC-NOR in the nor mutant did not restore the full ripening phenotype. Further analysis showed that the truncated NOR186 protein is located in the nucleus and binds to but does not activate the promoters of 1-aminocyclopropane-1-carboxylic acid synthase2 (SlACS2), geranylgeranyl diphosphate synthase2 (SlGgpps2), and pectate lyase (SlPL), which are involved in ethylene biosynthesis, carotenoid accumulation, and fruit softening, respectively. The activation of the promoters by the wild-type NOR protein can be inhibited by the mutant NOR186 protein. On the other hand, ethylene synthesis, carotenoid accumulation, and fruit softening were significantly inhibited in CR-NOR (CRISPR/Cas9-edited NAC-NOR) fruit compared with the wild-type, but much less severely affected than in the nor mutant, while they were accelerated in OE-NOR (overexpressed NAC-NOR) fruit. These data further indicated that nor is a gain-of-function mutation and NAC-NOR plays a significant role in ripening of wild-type fruit.

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