scholarly journals Ethylene-regulated gene expression in tomato fruit: characterization of novel ethylene-responsive and ripening-related genes isolated by differential display

1999 ◽  
Vol 18 (6) ◽  
pp. 589-600 ◽  
Author(s):  
Hicham Zegzouti ◽  
Brian Jones ◽  
Pierre Frasse ◽  
Christel Marty ◽  
Beatrice Maitre ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Display ◽  
Tomato Fruit ◽  
Regulated Gene Expression

scholarly journals 238.Determination of differentially displayed oxygen-sensitive genes in bovine blastocysts

2004 ◽  
Vol 16 (9) ◽  
pp. 238
Author(s):  
A. J. Harvey ◽  
M. Kirstein ◽  
A. Navarrete-Santos ◽  
K. L. Kind ◽  
B. Fischer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Display ◽  
Target Genes ◽  
Hypoxia Inducible Factor ◽  
Developmental Competence ◽  
Myelogenous Leukemia ◽  
Bovine Embryo ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Regulated Gene Expression

Oxygen-regulated gene expression in the bovine embryo contrasts markedly with that observed in the mouse. Under low (2%) oxygen moderate changes in gene expression are observed in the bovine blastocyst, compared with 3- to 4-fold increases in the mouse. We have determined that these moderate gene expression changes are most likely regulated by Hypoxia-Inducible Factor (HIF)-2 transcription factor activity in the bovine, in the absence of HIF1, although HIF2 target genes are largely unknown. The aim of this study was to screen, by differential display RT-PCR, for putative oxygen-regulated transcripts that might confer developmental competence in blastocysts cultured under varying oxygen atmospheres post compaction. In vitro-produced bovine blastocysts were generated using standard protocols. Compact morulae were randomly allocated to treatments under either 2%, 7% or 20% oxygen for 72 h from Day 5. Blastocyst RNA was isolated using TriReagent and samples were reverse transcribed using Superscript II. cDNA was amplified using 10-mer primers in reactions containing 32Pα-labelled dCTP. Resulting bands were detected by autoradiography, excised, purified and ligated into pGEMT vectors for transformation and sequencing. Seven clones were identified as having high homology with known sequences in GenBank. Real-time PCR was undertaken to confirm oxygen-regulation using Sybr green master mix. Myotrophin mRNA was significantly increased following 2% oxygen culture, compared with 20% cultured blastocysts (P�<�0.01), as was GLUT1 (P�<�0.01). The expression of anaphase-promoting complex showed a significant association with oxygen, being higher in 2% cultured blastocysts (P�<�0.05). Acetyl-coA-acetyltransferase I, chronic myelogenous leukemia tumor antigen (CML66), cyclin I, NADH dehydrogenase subunit 2 and ribonucleotide reductase M1, genes identified using differential display, were not altered by post compaction oxygen concentration. This study has identified potentially HIF2-specific regulated genes, and supports the hypothesis that reduced oxygen concentrations post-compaction may influence bovine embryo development through oxygen-regulated changes in gene expression.

Characterization of tomato fruit ripening and analysis of gene expression in F1 hybrids of the ripening inhibitor (rin) mutant

2005 ◽  
Vol 123 (3) ◽  
pp. 331-338 ◽  
Author(s):  
Mamiko Kitagawa ◽  
Hirotaka Ito ◽  
Takeo Shiina ◽  
Nobutaka Nakamura ◽  
Takahiro Inakuma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
F1 Hybrids ◽  
Tomato Fruit Ripening ◽  
Ripening Inhibitor ◽  
Rin Mutant

UVB-Regulated Gene Expression in Human Keratinocytes: Analysis by Differential Display

2003 ◽  
pp. 347-366 ◽  
Author(s):  
Harry Frank Abts ◽  
Thomas Welss ◽  
Kai Breuhahn ◽  
Thomas Ruzicka
Keyword(s):  
Gene Expression ◽  
Differential Display ◽  
Human Keratinocytes ◽  
Regulated Gene Expression

scholarly journals Development and Characterization of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression by Addition of Subtilin

2005 ◽  
Vol 71 (12) ◽  
pp. 8818-8824 ◽  
Author(s):  
Roger S. Bongers ◽  
Jan-Willem Veening ◽  
Maarten Van Wieringen ◽  
Oscar P. Kuipers ◽  
Michiel Kleerebezem
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Fluorescent Protein ◽  
Expression System ◽  
Functional Characterization ◽  
Industrial Applications ◽  
Wild Type ◽  
Control Of Gene Expression ◽  
Regulated Gene Expression

ABSTRACT A system for subtilin-regulated gene expression (SURE) in Bacillus subtilis that is based on the regulatory module involved in cell-density-dependent control of the production of subtilin is described. An integration vector for introduction of the essential sensor-regulator couple spaRK into the amyE locus of the B. subtilis chromosome and a B. subtilis 168-derived production host in which the spaRK genes were functionally introduced were constructed. Furthermore, several expression plasmids harboring the subtilin-inducible wild-type spaS promoter or a mutated derivative of this promoter were constructed, which facilitated both transcriptional and translational promoter-gene fusions. Functional characterization of both spaS promoters and the cognate expression host could be performed by controlled overproduction of the β-glucuronidase (GusA) and green fluorescent protein (GFP) reporters. Both spaS promoters exhibited very low levels of basal expression, while extremely high levels of expression were observed upon induction with subtilin. Moreover, the level of expression depended directly on the amount of inducer (subtilin) used. The wild-type spaS promoter appeared to be more strictly controlled by the addition of subtilin, while the highest levels of expression were obtained when the mutated spaS promoter was used. Induction by subtilin led to 110- and 80-fold increases in GusA activity for the spaS promoter and its mutant derivative, respectively. Since the SURE system has attractive functional characteristics, including promoter silence under noninducing conditions and a controlled and high level of expression upon induction, and since it is not subject to catabolite control, we anticipate that it can provide a suitable expression system for various scientific and industrial applications.

scholarly journals 347 ETHYLENE-INDUCED GENE EXPRESSION DURING FLOWER PETAL ABSCISSION OF ZONAL GERANIUM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 480d-480
Author(s):  
David G. Clark ◽  
Kathleen B. Evensen
Keyword(s):  
Gene Expression ◽  
Cdna Library ◽  
Plant Development ◽  
Differential Screening ◽  
Flower Petal ◽  
Pelargonium Xhortorum ◽  
Ethylene Action ◽  
Regulated Gene Expression ◽  
Identification And Characterization

Ethylene-regulated gene expression is being studied in several plant systems, but the exact mechanism of ethylene action during plant development and senescence is poorly understood. When geranium (Pelargonium Xhortorum) flowers are exposed to 1 μ1/L of ethylene gas for 1 hour, petals begin to abscise within 60-90 minutes from the start of treatment, The rapidity of the response implies that it must be very direct. We now demonstrate that ethylene acts at the level of message accumulation. We have constructed a cDNA library from mRNA isolated from ethylene-treated geranium gynoecia. Ethylene-induced clones have been isolated by differential screening of this library with cDNA probes synthesized from ethylene-treated and untreated geranium gynoecia mRNA. Identification and characterization of these clones will be discussed.

Down-regulated gene expression of cold acclimated Poncirus trifoliata

2005 ◽  
Vol 85 (2) ◽  
pp. 417-424 ◽  
Author(s):  
Can-Kui Zhang ◽  
Ping Lang ◽  
Robert C. Ebel ◽  
Fenny Dane ◽  
Narendra K Singh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cold Acclimation ◽  
Differential Display ◽  
Regulation Of Gene Expression ◽  
Freeze Tolerance ◽  
Poncirus Trifoliata ◽  
Close Relative ◽  
Rt Pcr ◽  
Control Of Gene Expression ◽  
Regulated Gene Expression

Citrus sp. are important commercial fruit crops throughout the world that are occasionally devastated by subfreezing temperatures. Poncirus trifoliata (maximum freeze tolerance of -26°C) is a close relative of commercial Citrus sp. (maximum freeze tolerance of -10°C) that has been used in breeding programs to develop more cold-hardy genotypes and as a rootstock to enhance freeze tolerance of the scion. Species with greater freeze tolerance vary in gene expression during cold acclimating temperatures. mRNA differential display (DDRT-PCR) and quantitative relative RT-PCR were used to study down regulation of gene expression in intact P. trifoliata exposed to a gradual cold acclimation regime to enhance our understanding of the mechanism that makes this specie so freeze tolerant. Six down-regulated genes were isolated and sequenced. These down-regulated genes showed high homology to the following known genes: chlorophyll a/b binding protein, photosystem II OEC 23, carbonic anhydrase, tumor related protein, pyrrolidone-carboxylate peptid ase and β-galactosidase. Photoprotection and the global control of gene expression related to photosynthesis appear to be important mechanisms for cold acclimation of P. trifoliata. Key words: Differential display, down-regulated genes, Poncirus trifoliata, cold acclimation and quantitative relative RT-PCR

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