Multi-omics analysis reveals specific modifications associated with reduced chilling injury in bell pepper fruit by methyl jamonate

An emerging theory contends that chilling injury is due to oxidative damage resulting from the metabolic generation of active oxygen species. Mitochondria were isolated from chilling-sensitive (CS) and from conditioned chilling-resistant (CR) bell pepper fruit and their ability to generate superoxide determined by measuring the formation of adrenochrome from epinephrine. Mitochondria from CS fruit were sensitive to cyanide and produced superoxide when supplied with NADH, succinate, or malate-pyruvate. Mitochondria from CR fruit were insensitive to cyanide and sensitive to SHAM and produced little superoxide when supplied with respiratory substrates. ATP enhanced the production of superoxide and ADP reduced the production. Results suggest that the mitochondria are a major source of superoxide in CS plant tissue and the presence of the alternative pathway reduces the production of superoxide.

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Diphenylamine reduces chilling injury of green bell pepper fruit

Postharvest Biology and Technology ◽

10.1016/s0925-5214(01)00144-2 ◽

2002 ◽

Vol 25 (1) ◽

pp. 41-48 ◽

Cited By ~ 25

Author(s):

Albert C. Purvis

Keyword(s):

Chilling Injury ◽

Bell Pepper ◽

Pepper Fruit

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Growth-regulator-induced Alleviation of Chilling Injury in Green and Red Bell Pepper Fruit during Storage

HortScience ◽

10.21273/hortsci.30.3.558 ◽

1995 ◽

Vol 30 (3) ◽

pp. 558-559 ◽

Cited By ~ 6

Author(s):

Susan Lurie ◽

Reuven Ronen ◽

Beny Aloni

Keyword(s):

Growth Regulators ◽

Low Temperatures ◽

Chilling Injury ◽

Bell Pepper ◽

Fruit Morphology ◽

Full Bloom ◽

Affected Plant ◽

Pepper Fruit ◽

Red Peppers ◽

Whole Plants

Bell pepper (Capsicum annuum L.) plants were sprayed at full bloom with paclobutrazol (50 and 100 mg·liter–1), uniconazole (20 and 50 mg·liter–1), or mefluidide (20 and 50 mg·liter–1). Mature-green and red fruit were harvested 4 and 8 weeks after spraying, respectively. Paclobutrazol and uniconazole, but not mefluidide, affected plant growth and fruit morphology. All three growth regulators alleviated chilling injury that developed on green and red peppers after 28 days at 2C. Potassium leakage was lower from tissue disks, and weight loss less, from treated fruit than from control fruit. Ethylene and CO2 production at 20C were similar in control and paclobutrazol-treated fruit after 28 days at 2C. These results are consistent with previous findings that showed these growth regulators are able to increase tolerance to low temperatures in whole plants. Chemical names used: N-[2,4-dimethyl-5-trifluoromethyl sulfonyl amino phenyl acetamide] (mefluidide); (2RS, 3RS) 1-(4-chlorophenyl)-4,4 dimethyl-2-(1,2,4-triazol-1-yl)-pentan-3-ol (paclobutrazol); (E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4 triazol-1-yl)-pentan-3-ol (uniconazole).

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