Functional Characterization of the 5′-upstream Region of MTT5 Metallothionein Gene from Tetrahymena thermophila

Protist ◽  
2010 ◽  
Vol 161 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Alessia Formigari ◽  
Francesco Boldrin ◽  
Gianfranco Santovito ◽  
Donna Cassidy-Hanley ◽  
Theodore G. Clark ◽  
...  
Keyword(s):  
Tetrahymena Thermophila ◽  
Functional Characterization ◽  
Upstream Region ◽  
Metallothionein Gene

scholarly journals Identification and Functional Characterization of Protest SWI/SNF and ISWI Complexes

2021 ◽  
Author(s):  
Alejandro Saettone Chipana
Keyword(s):  
Chromatin Remodeling ◽  
Tetrahymena Thermophila ◽  
Affinity Purification ◽  
Functional Characterization ◽  
Peptide Array ◽  
Interacting Proteins ◽  
Silent Chromatin ◽  
Lysine Residues ◽  
Chromatin Remodeling Complexes

The thesis aims to identify and initiate functional characterization of the SWI/SNF and ISWI complexes in Tetrahymena thermophila. Through affinity purification of the conserved subunit Snf5 followed by mass spectrometry (AP-MS), I identified the first SWI/SNF complex in protists. One of the subunits I found is a small bromodomain containing protein named Ibd1. Through AP-MS of Ibd1 I found Ibd1 is versatile and interacts with several additional chromatin remodeling complexes. Bromodomains are known to have affinity for acetylated lysine residues within proteins such as histones. A peptide array experiment suggests that Ibd1 also has affinity for acetylated chromatin. Indirect immunofluorescence (IF) of Ibd1 hints at a role in transcription. My analysis of Tetrahymena Iswi1 shows expression during meiosis, vegetative growth and starvation. IF data shows its localization is consistent with Iswi1 function in mitosis/meiosis or maintenance of silent chromatin. AP-MS of ISW1 discovered several interacting proteins of unknown function.

In vivo functional characterization of an ecdysone response enhancer in the proximal upstream region of the Fbp1 gene of D. melanogaster

1993 ◽  
Vol 44 (2-3) ◽  
pp. 123-138 ◽  
Author(s):  
Monique Laval ◽  
Francine Pourrain ◽  
Jean Deutsch ◽  
Jean-Antoine Lepesant
Keyword(s):  
Functional Characterization ◽  
Upstream Region ◽  
Fbp1 Gene

scholarly journals Functional Characterization of a Type 4 Metallothionein Gene (CsMT4) in Cucumber

2019 ◽  
Vol 5 (3) ◽  
pp. 120-128 ◽  
Author(s):  
Ling Duan ◽  
Jingwen Yu ◽  
Li Xu ◽  
Ping Tian ◽  
Xin Hu ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Metallothionein Gene

Expression and functional characterization of a C-7 cholesterol desaturase from Tetrahymena thermophila in an insect cell line

Steroids ◽  
2015 ◽  
Vol 96 ◽  
pp. 132-139 ◽  
Author(s):  
Tomas J. Poklepovich ◽  
Nicolás Urtasun ◽  
María V. Miranda ◽  
Alejandro D. Nusblat ◽  
Clara B. Nudel
Keyword(s):  
Cell Line ◽  
Insect Cell ◽  
Tetrahymena Thermophila ◽  
Functional Characterization ◽  
Insect Cell Line

scholarly journals Structural and functional characterization of the 5' upstream region of a glutamine synthetase gene from Scots pine

2002 ◽  
Vol 59 (5-6) ◽  
pp. 669-673 ◽  
Author(s):  
Concepci�n Avila ◽  
Francisco R. Cant�n ◽  
Pilar Barnestein ◽  
Mar�a-Fernanda Su�rez ◽  
Pierre Marraccini ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Scots Pine ◽  
Functional Characterization ◽  
Upstream Region ◽  
Glutamine Synthetase Gene

Isolation and Functional Characterization of the 5′-Upstream Region of Mouse P/Q-Type Ca2+ Channel α1A Subunit Gene

1999 ◽  
Vol 260 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Eiki Takahashi ◽  
Yoshiyuki Murata ◽  
Tohru Oki ◽  
Norimasa Miyamoto ◽  
Yasuo Mori ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Upstream Region ◽  
Subunit Gene

Cloning and functional characterization of a novel metallothionein gene in Antarctic sea-ice yeast (Rhodotorula mucilaginosa )

2019 ◽  
Vol 59 (9) ◽  
pp. 879-889 ◽  
Author(s):  
Guangfeng Kan ◽  
Yun Ju ◽  
Ying Zhou ◽  
Cuijuan Shi ◽  
Yongping Qiao ◽  
...  
Keyword(s):  
Sea Ice ◽  
Functional Characterization ◽  
Rhodotorula Mucilaginosa ◽  
Metallothionein Gene ◽  
Antarctic Sea Ice
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