X-ray microbeam measurement with radiophotoluminescent glass plate for single cell irradiation

This article describes carbon-nanotube based X-ray technologies for medical research and clinical applications, including an X-ray source, microfocus X-ray tube, microcomputed tomography scanner, stationary digital breast tomosynthesis, microradiotherapy system, and single-cell irradiation system. It first examines electron field emission from carbon nanotubes before discussing carbon-nanotube field emission electron and X-ray technologies in greater detail. It highlights the enormous promise of these systems in commercial and research application for the future in diagnostic medical imaging; in-vivo imaging of small-animal modelsfor pre-clinical cancer studies; security screening; industrial inspection; cancer radiotherapy of small-animal models for pre-clinical cancer studies; and basic cancer research using single-cell irradiation.

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Microdosimetric Characteristics of Micro X-Ray Beam for Single Cell Irradiation

IEEE Nuclear Science Symposium Conference Record, 2005 ◽

10.1109/nssmic.2005.1596963 ◽

2006 ◽

Author(s):

T. Kuchimaru ◽

F. Sato ◽

Y. Higashino ◽

K. Shimizu ◽

Y. Kato ◽

...

Keyword(s):

Single Cell ◽

X Ray ◽

Cell Irradiation

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Simulation of single cell irradiation by proton beams using TILDA-V

Journal of Physics Conference Series ◽

10.1088/1742-6596/1412/21/212004 ◽

2020 ◽

Vol 1412 ◽

pp. 212004

Author(s):

M E Alcocer-Ávila ◽

M A Quinto ◽

J M Monti ◽

P F Weck ◽

O A Fojón ◽

...

Keyword(s):

Single Cell ◽

Proton Beams ◽

Cell Irradiation

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Determination of carbon-to-nitrogen ratio in the filamentous and heterocystous cyanobacterium Anabaena sp. PCC 7120 with single-cell soft X-ray imaging

Journal of Physics Conference Series ◽

10.1088/1742-6596/849/1/012005 ◽

2017 ◽

Vol 849 ◽

pp. 012005

Author(s):

T Teramoto ◽

M Yoshimura ◽

C Azai ◽

K Terauchi ◽

T Ohta

Keyword(s):

Single Cell ◽

Heterocystous Cyanobacterium ◽

X Ray ◽

Carbon To Nitrogen Ratio ◽

X Ray Imaging ◽

Cyanobacterium Anabaena ◽

Anabaena Sp ◽

Nitrogen Ratio ◽

Pcc 7120

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Single-cell resolution in high-resolution synchrotron X-ray CT imaging with gold nanoparticles

Journal of Synchrotron Radiation ◽

10.1107/s1600577513029007 ◽

2013 ◽

Vol 21 (1) ◽

pp. 242-250 ◽

Cited By ~ 17

Author(s):

Elisabeth Schültke ◽

Ralf Menk ◽

Bernd Pinzer ◽

Alberto Astolfo ◽

Marco Stampanoni ◽

...

Keyword(s):

Gold Nanoparticles ◽

Single Cell ◽

Brain Tumour ◽

Ex Vivo ◽

Small Animal ◽

C6 Glioma Cells ◽

X Ray ◽

Local Tomography ◽

The Brain

Gold nanoparticles are excellent intracellular markers in X-ray imaging. Having shown previously the suitability of gold nanoparticles to detect small groups of cells with the synchrotron-based computed tomography (CT) technique bothex vivoandin vivo, it is now demonstrated that even single-cell resolution can be obtained in the brain at leastex vivo. Working in a small animal model of malignant brain tumour, the image quality obtained with different imaging modalities was compared. To generate the brain tumour, 1 × 105C6 glioma cells were loaded with gold nanoparticles and implanted in the right cerebral hemisphere of an adult rat. Raw data were acquired with absorption X-ray CT followed by a local tomography technique based on synchrotron X-ray absorption yielding single-cell resolution. The reconstructed synchrotron X-ray images were compared with images obtained by small animal magnetic resonance imaging. The presence of gold nanoparticles in the tumour tissue was verified in histological sections.

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