In vitro exposure of porcine granulosa cells to the phytoestrogens genistein and daidzein: Effects on the biosynthesis of reproductive steroid hormones

2007 ◽  
Vol 24 (3-4) ◽  
pp. 317-325 ◽  
Author(s):  
U TIEMANN ◽  
F SCHNEIDER ◽  
J VANSELOW ◽  
W TOMEK
Keyword(s):  
Steroid Hormones ◽  
Granulosa Cells ◽  
Porcine Granulosa Cells ◽  
In Vitro Exposure

RGD-mediated adhesion of porcine granulosa cells modulates their differentiation response to FSH in vitro

1995 ◽  
Vol 83 (2-3) ◽  
pp. 169-177 ◽  
Author(s):  
Béatrice Goxe ◽  
Jacques E. Flechon ◽  
Solange Delasalle ◽  
Roland Salesse
Keyword(s):  
Granulosa Cells ◽  
Porcine Granulosa Cells

COMPARISON OF THE STIMULATORY EFFECTS OF OVINE, PORCINE AND HUMAN FOLLICLE-STIMULATING HORMONE AND OF OVINE AND HUMAN LUTEINIZING HORMONE ON THE ACCUMULATION OF CYCLIC AMP BY PORCINE GRANULOSA CELLS

1979 ◽  
Vol 80 (1) ◽  
pp. 9-20 ◽  
Author(s):  
ADA M. LINDSEY ◽  
CORNELIA P. CHANNING
Keyword(s):  
Cyclic Amp ◽  
Granulosa Cells ◽  
Incubation Medium ◽  
Follicle Stimulating Hormone ◽  
Similar Response ◽  
Intracellular Accumulation ◽  
Porcine Granulosa Cells ◽  
Tenfold Increase ◽  
Incubation Periods

The effects of ovine, porcine and human FSH, and ovine and human LH on the accumulation of cyclic AMP by porcine granulosa cells obtained from follicles at various stages of maturation were investigated. During incubation periods of 15 min, 10 μg ovine FSH pretreated with antiserum to LH or 10 μg human FSH resulted in an 11- to 18-fold, five-to ninefold, and less than a twofold increase in intracellular accumulation of cyclic AMP by granulosa cells from small (1–2 mm), medium (3–5 mm) and large (6–12 mm) follicles respectively. Similar patterns of response occurred with addition of porcine FSH. After incubation for 30 and 60 min with ovine, porcine or human FSH, significant accumulation of cyclic AMP in the incubation medium occurred with cells obtained from small and medium-sized follicles. After 60 min of incubation with FSH the accumulation of cyclic AMP in the incubation medium exceeded the intracellular cyclic AMP levels in granulosa cells from small and medium-sized follicles. During incubation periods of 15 min, 1·0 μg ovine LH resulted in less than a twofold, a fourfold and greater than a tenfold increase in intracellular accumulation of cyclic AMP by granulosa cells from small, medium and large follicles respectively. Addition of human LH brought about a similar response. Incubation periods of 30 and 60 min with 1·0 μg ovine or human LH resulted in significant accumulation of cyclic AMP in the incubation medium by granulosa cells from large follicles; cyclic AMP content in the incubation medium was greater after 60 min compared with 30 min of incubation. It was concluded that ovine FSH pretreated with an antiserum to LH had similar effects on cyclic AMP levels as did purified human and porcine FSH, and that the stimulatory effects of the less pure ovine FSH were probably not due to an impurity in the FSH preparation. Porcine granulosa cells obtained from small follicles should be suitable as an in-vitro FSH bioassay while granulosa cells obtained from large follicles should be suitable as an in-vitro LH bioassay.

scholarly journals Does 2-hydroxyflutamide Inhibit Apoptosis in Porcine Granulosa Cells? ^|^mdash; An In Vitro Study

2012 ◽  
Vol 58 (4) ◽  
pp. 438-444 ◽  
Author(s):  
Malgorzata DUDA ◽  
Malgorzata DURLEJ ◽  
Malgorzata KNET ◽  
Katarzyna KNAPCZYK-STWORA ◽  
Zbigniew TABAROWSKI ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Porcine Granulosa Cells

Prolactin, LH, and estradiol-17β in utilization of lipoprotein substrate by porcine granulosa cells in vitro

1988 ◽  
Vol 66 (5) ◽  
pp. 561-566 ◽  
Author(s):  
K. Rajkumar ◽  
P. Klingshorn ◽  
P. J. Chedrese ◽  
B. D. Murphy
Keyword(s):  
Granulosa Cell ◽  
Cell Cultures ◽  
Granulosa Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Porcine Granulosa Cells ◽  
Progesterone Synthesis ◽  
Serum Free Conditions

Porcine granulosa cells cultured under serum free conditions responded by increased progesterone secretion to the addition of the leuteotropic hormones, LH, prolactin, and estradiol. Provision of extracellular substrate for steroidogenesis in the form of porcine high density lipoprotein or low density lipoprotein enhanced progesterone accumulation by granulosa cell cultures. Estradiol, LH, and prolactin all greatly increased progesterone accumulation in the presence of either high or low density lipoproteins. Increases in progesterone accumulation following addition of prolactin or LH in combination with estradiol suggested the presence of a synergistic interaction among leuteotropins. Pre-exposure of granulosa cell cultures to estradiol increased the subsequent stimulatory effect of prolactin on lipoprotein utilization. It is concluded that all three leuteotropins function to enhance and may interact in the utilization of extracellular lipoprotein substrate for progesterone synthesis.

Prostaglandins PGE and PGF in human ovarian follicles: endogenous contents and in vitro formation by theca and granulosa cells

1981 ◽  
Vol 97 (4) ◽  
pp. 543-550 ◽  
Author(s):  
Vasant V. Patwardhan ◽  
André Lanthier
Keyword(s):  
Arachidonic Acid ◽  
Menstrual Cycle ◽  
Granulosa Cells ◽  
Physiological Role ◽  
In Vitro Exposure ◽  
Intrinsic Capacity ◽  
Human Ovaries ◽  
Human Ovarian Follicles

Abstract. The possible role of prostaglandins of the PGE and PGF series in the follicular compartment of human ovaries or the capacity of that tissue to form them is not well defined. In the present experiment we have examined 1) the endogenous concentrations of PGE and PGF in follicles of human ovaries at various stages of the menstrual cycle, 2) the capacity of separated theca and granulosa to form PGE and PGF in vitro in the presence of substrate arachidonic acid and 3) the possible modulation of that capacity by previous in vitro exposure to hCG (10 IU) alone or in combination with hMG (5 IU). PGE and PGF were determined by radioimmunoassay. Follicles from all stages of the cycle were found to contain measurable amounts of PGE (0.10–3.75 ng/follicle) and PGF (0.13–1.11 ng/follicle). These compounds were localized more in the theca than the granulosa cells. On a per follicle basis theca showed more capacity to form PGE and PGF in vitro than the corresponding granulosa cells. However, exposure to gonadotrophins stimulated PGE and PGF formation in the granulosa cells and not in the theca. The presence of PGE and PGF in the follicles indicates a physiological role for these compounds in that tissue. Although thecal tissue showed a greater intrinsic capacity to form PGE and PGF, the contribution of granulosa cells may be more important under acute gonadotrophin stimulation.

scholarly journals Effects of transferrin on aromatase activity in porcine granulosa cells in vitro.

2009 ◽  
Vol 46 (4) ◽  
Author(s):  
Małgorzata Durlej ◽  
Małgorzata Duda ◽  
Katarzyna Knapczyk ◽  
Maria Słomczyńska
Keyword(s):  
Granulosa Cells ◽  
Aromatase Activity ◽  
Porcine Granulosa Cells

scholarly journals Transient Transfection of Porcine Granulosa Cells after 3D Culture in Barium Alginate Capsules

2005 ◽  
Vol 18 (4) ◽  
pp. 677-681 ◽  
Author(s):  
E. Benzoni ◽  
M.L. Torre ◽  
M. Faustini ◽  
S. Stacchezzini ◽  
F. Cremonesi ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Fluorescent Protein ◽  
3D Culture ◽  
Transient Transfection ◽  
Necessary Condition ◽  
In Vitro Toxicity ◽  
Culture Systems ◽  
Porcine Granulosa Cells ◽  
Alginate Capsules

Three-dimensional culture systems in barium alginate capsules can be employed to maintain primary granulosa cells in an undifferentiated state for almost 6 days. This is due to a self-organization of cells in a pseudofollicular structure. The transfection of primary granulosa cells is a necessary condition when employing these culture systems for several purposes, for example as an in vitro toxicity test or the development of oocytes or zygotes. In this work, the feasibility of two transient transfection techniques (liposome-mediated and electroporation) was assessed in primary porcine granulosa cells after a 6-day culture in an artificial extracellular matrix (barium alginate membrane). Human recombinant green fluorescent protein was chosen as a molecular readout, and protein expression was assessed after 48 hours from transfection. Liposome-mediated transfection gave low transfection levels, with increasing yields from 2 to 12 μgDNA/ml of medium; the maximum percentage (85.7%) was reached at 12 μgDNA/ml of medium. Electroporation-mediated transfection yields were higher: the best results (81.7% of transfected cells) were achieved with two 50V pulses and 12 μg/ml DNA. The application of a single or double pulse (50V) at 4 mgDNA/ml gave negligible results. These results indicate that primary granulosa cell cultured in barium alginate capsules can be transfected by electroporation with high transfection yields.

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