Detection of biosynthetic gene and phytohormone production by endophytic actinobacteria associated with Solanum lycopersicum and their plant-growth-promoting effect

Root-knot nematodes (RKN), Meloidogyne sp. hinders functioning of crops and causes global losses in terms of productivity and yield. Meloidogyne sp. are microscopic, obligatory endoparasites with ubiquitous distribution in different parts of the world. Taking into consideration these aspects, the present study was conducted to explore nematicidal activity of the Streptomyces hydrogenans strain DH-16 against M. incognita to regulate its pathogenicity in plants. In-vitro experimentation revealed that pretreated seeds with solvent and culture supernatant lowered root galls in infested plants and promoted growth of Solanum lycopersicum seedlings, revealed through the morphological analysis. Additionally, antioxidative defense responses were induced with microbes. However, oxidative stress markers were considerably reduced after microbial inoculations. Apart from this, secondary metabolites were assessed and modulated in RKN infested plants on microbial supplementations. Confocal studies evaluated glutathione accumulation within root apices and its enhancement was directly proportional to defense responses. Therefore, the current study concluded the role of S. hydrogenans in stimulating antioxidant potential against RKN along with growth promoting aids. Thus, the outcome of the current study endorses that metabolites produced by S. hydrogenans can be used as safe biocontrol agents against M. incognita and also as plant growth promoting agents.

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Effect of Azospirillum brasilense and mycorrhizal soil fungi on topinambur grown in a greenhouse

Journal of Applied Biotechnology & Bioengineering ◽

10.15406/jabb.2021.08.00261 ◽

2021 ◽

Vol 8 (4) ◽

pp. 104-110

Author(s):

Di Barbaro Gabriela ◽

Andrada Horacio ◽

Batallan Morales Silvana ◽

Espeche Acosta Eliana ◽

Rizo Melisa ◽

...

Keyword(s):

Plant Growth ◽

Mycorrhizal Fungi ◽

Azospirillum Brasilense ◽

Soil Fungi ◽

Jerusalem Artichoke ◽

Promoting Effect ◽

Greenhouse Production ◽

Plant Growth Promoting ◽

Growth Promoting ◽

The Moment

To determine the effect of Azospirillum brasilense and soil mycorrhizal fungi on the nutrition of the Jerusalem artichoke crop (Helianthus tuberosus L.), evaluations of agronomic parameters and the health status of the plants were carried out, under greenhouse conditions. The tests were carried out, at the moment of the implantation of the culture: the tubers were inoculated with A. brasilense and with native mycorrhizal fungi, generating four treatments including the control and the co-inoculation of the consortium of the microorganisms under study (T0: control or control without inoculation; T1: inoculation with native A. brasilense; T2: inoculation with native mycorrhizal fungi and T3: joint inoculation with A. brasilense and native mycorrhizal fungi. The results indicate that co-inoculation with A. brasilense and with native mycorrhizal fungi increased plant growth in height, leaf area, biomass, dry matter, and yields significantly in greenhouse production. It was determined that the application of the selected microorganisms has a plant growth-promoting effect, increasing the productivity of cultivated topinambur in the greenhouse

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