Compared with the G-quadruplex/ThT fluorescent system, parallel [TG(GA)3]n-dsDNA/ThT is a stable and strong fluorescent indicator for label-free biosensing.
A simple fluorescence based biosensor for label-free detection of uracil DNA glycosylase activity based on G-quadruplex formation using a thioflavin T probe is reported.
Fluorometric detection of microRNA using Rolling Circle Amplification generating tandem G-quadruplex (GQ-RCA). Target miRNA triggers the GQ-RCA reaction generating tandem repeats of the G-quadruplex, resulting in enhanced Thioflavin T fluorescence.