Label-free fluorescence assay for rapid detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates

2017 ◽  
Vol 9 (20) ◽  
pp. 3055-3060 ◽  
Author(s):  
Kefeng Wu ◽  
Changbei Ma ◽  
Haisheng Liu ◽  
Hailun He ◽  
Weimin Zeng ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Rnase H ◽  
Fluorescence Assay ◽  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex

A simple fluorescence based biosensor for label-free detection of RNase H activity based on Tb3+-induced G-quadruplex conjugates is reported.

A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Label Free ◽  
Activity Assay ◽  
G Quadruplex ◽  
Catalytic Hairpin Assembly ◽  
Amplification Strategy

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.

scholarly journals Comparison of DNAzyme activity for the development of an immobilized heme sensor

MRS Advances ◽  
2018 ◽  
Vol 3 (26) ◽  
pp. 1491-1496
Author(s):  
Natalie Hughes ◽  
Nancy Nguyen ◽  
Deanna-Kaye Daley ◽  
Justin Grennell ◽  
Amira Gee ◽  
...  
Keyword(s):  
Self Assembly ◽  
Point Of Care ◽  
Label Free ◽  
Detection Range ◽  
Chemical Complexity ◽  
Detection Strategy ◽  
Detection Approach ◽  
Label Free Detection ◽  
G Quadruplex ◽  
Care Systems

ABSTRACTPoint-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

scholarly journals Label Free Detection of Adenosine Based on Hemin/G-Quadruplex Peroxidase-Mimicking DNAzyme as Bioelectrocatalyst

2013 ◽  
Vol 25 (18) ◽  
pp. 10541-10545 ◽  
Author(s):  
Yu Li ◽  
Sen Li ◽  
Ge-Qing Xia ◽  
Yuansheng Wang
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex

Cationic-perylene-G-quadruplex complex based fluorescent biosensor for label-free detection of Pb 2+

Talanta ◽  
2016 ◽  
Vol 149 ◽  
pp. 98-102 ◽  
Author(s):  
Xu-Hua Zhao ◽  
Liang Gong ◽  
Yuan Wu ◽  
Xiao-Bing Zhang ◽  
Jun Xie
Keyword(s):  
Label Free ◽  
Fluorescent Biosensor ◽  
Label Free Detection ◽  
G Quadruplex

scholarly journals Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules

2020 ◽  
Vol 12 (21) ◽  
pp. 2674-2681
Author(s):  
Fatemeh Shafiei ◽  
Kathleen McAuliffe ◽  
Yousef Bagheri ◽  
Zhining Sun ◽  
Qikun Yu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Rapid Detection ◽  
Rna Aptamer ◽  
Label Free ◽  
Target Analytes ◽  
Label Free Detection

A paper-based portable fluorogenic RNA sensor for the selective, sensitive, and rapid detection of target analytes.

scholarly journals Label-free Detection of Zn2+ Based on G-quadruplex

2015 ◽  
Vol 31 (10) ◽  
pp. 1041-1045 ◽  
Author(s):  
Yahui GUO ◽  
Yan SUN ◽  
Xiaoqiang SHEN ◽  
Kunchi ZHANG ◽  
Jiming HU ◽  
...  
Keyword(s):  
Label Free ◽  
Label Free Detection ◽  
G Quadruplex
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