Riboswitches: small-molecule recognition by gene regulatory RNAs

2007 ◽  
Vol 17 (3) ◽  
pp. 273-279 ◽  
Author(s):  
Thomas E Edwards ◽  
Daniel J Klein ◽  
Adrian R Ferré-D’Amaré
Keyword(s):  
Small Molecule ◽  
Molecule Recognition ◽  
Gene Regulatory ◽  
Regulatory Rnas

scholarly journals Development and Implementation of an HTS-Compatible Assay for the Discovery of Selective Small-Molecule Ligands for Pre-microRNAs

2017 ◽  
Vol 23 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Daniel A. Lorenz ◽  
Steve Vander Roest ◽  
Martha J. Larsen ◽  
Amanda L. Garner
Keyword(s):  
Small Molecules ◽  
Natural Product ◽  
Small Molecule ◽  
High Throughput ◽  
High Throughput Screening ◽  
Two Dimensional ◽  
Small Molecule Ligands ◽  
Gene Regulatory ◽  
Regulatory Rnas ◽  
Target Rna

microRNAs (miRNAs) are small gene regulatory RNAs, and their expression has been found to be dysregulated in a number of human diseases. To facilitate the discovery of small molecules capable of selectively modulating the activity of a specific miRNA, we have utilized new high-throughput screening technology targeting Dicer-mediated pre-miRNA maturation. Pilot screening of ~50,000 small molecules and ~33,000 natural product extract libraries against pre-miR-21 processing indicated the potential of our assay for this goal, yielding a campaign Z′ factor of 0.52 and an average plate signal-to-background (S/B) ratio of 13. Using two-dimensional screening against a second pre-miRNA, pre-let-7d, we evaluated the selectivity of confirmed hits. The results presented demonstrate how high-throughput screening can be used to identify selective small molecules for a target RNA.

Graphene Oxide/Perylene-Aniline Electrochemiluminescence Platform for Protein Detection Based on Molecule Recognition

2021 ◽  
Author(s):  
Peng Yin ◽  
Wei Zhang ◽  
Lei Shang ◽  
Rong-Na Ma ◽  
Liping Jia ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Folic Acid ◽  
Small Molecule ◽  
Folate Receptor ◽  
Protein Detection ◽  
Single Strand ◽  
Molecule Recognition

Most biosensors for protein folate receptor(FR) detection based on small molecule folic acid(FA) recognition usually introduced FA linked single strand DNA(FA-ssDNA) and nuclease to promote sensitivity, which increased expenses and...

scholarly journals Interplay of cysteine exposure and global protein dynamics in small-molecule recognition by a regulator of G-protein signaling protein

2018 ◽  
Vol 87 (2) ◽  
pp. 146-156 ◽  
Author(s):  
Mohammadjavad Mohammadi ◽  
Hossein Mohammadiarani ◽  
Vincent S. Shaw ◽  
Richard R. Neubig ◽  
Harish Vashisth
Keyword(s):  
Protein Dynamics ◽  
G Protein ◽  
Small Molecule ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Signaling Protein ◽  
Molecule Recognition

scholarly journals A Platform for the Detection of Trypanosomes via Selective Small Molecule Recognition

2011 ◽  
Vol 2 (7) ◽  
pp. 555-558 ◽  
Author(s):  
Ellen D. Beaulieu ◽  
Lori L. Olson ◽  
Mary J. Tanga
Keyword(s):  
Small Molecule ◽  
Molecule Recognition

scholarly journals Molecular Recognition of the Hybrid-Type G-Quadruplexes in Human Telomeres

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1578 ◽  
Author(s):  
Guanhui Wu ◽  
Luying Chen ◽  
Wenting Liu ◽  
Danzhou Yang
Keyword(s):  
Small Molecule ◽  
Solution Structure ◽  
Structural Information ◽  
Rational Drug Design ◽  
Hybrid Type ◽  
G4 Dna ◽  
Rational Drug ◽  
G Quadruplex ◽  
Molecule Recognition ◽  
Dna Secondary Structures

G-quadruplex (G4) DNA secondary structures formed in human telomeres have been shown to inhibit cancer-specific telomerase and alternative lengthening of telomere (ALT) pathways. Thus, human telomeric G-quadruplexes are considered attractive targets for anticancer drugs. Human telomeric G-quadruplexes are structurally polymorphic and predominantly form two hybrid-type G-quadruplexes, namely hybrid-1 and hybrid-2, under physiologically relevant solution conditions. To date, only a handful solution structures are available for drug complexes of human telomeric G-quadruplexes. In this review, we will describe two recent solution structural studies from our labs. We use NMR spectroscopy to elucidate the solution structure of a 1:1 complex between a small molecule epiberberine and the hybrid-2 telomeric G-quadruplex, and the structures of 1:1 and 4:2 complexes between a small molecule Pt-tripod and the hybrid-1 telomeric G-quadruplex. Structural information of small molecule complexes can provide important information for understanding small molecule recognition of human telomeric G-quadruplexes and for structure-based rational drug design targeting human telomeric G-quadruplexes.

Membrane Activation:  Selective Vesicle Fusion via Small Molecule Recognition

2006 ◽  
Vol 128 (45) ◽  
pp. 14430-14431 ◽  
Author(s):  
Yun Gong ◽  
Yumei Luo ◽  
Dennis Bong
Keyword(s):  
Small Molecule ◽  
Vesicle Fusion ◽  
Molecule Recognition

scholarly journals Small Molecule Recognition and Tools to Study Modulation of r(CGG)exp in Fragile X-Associated Tremor Ataxia Syndrome

2016 ◽  
Vol 11 (9) ◽  
pp. 2456-2465 ◽  
Author(s):  
Wang-Yong Yang ◽  
Fang He ◽  
Rita L. Strack ◽  
Seok Yoon Oh ◽  
Michelle Frazer ◽  
...  
Keyword(s):  
Small Molecule ◽  
Fragile X ◽  
Molecule Recognition ◽  
Ataxia Syndrome

scholarly journals Native Serotonin Membrane Receptors Recognize 5-Hydroxytryptophan-Functionalized Substrates: Enabling Small-Molecule Recognition

2010 ◽  
Vol 1 (7) ◽  
pp. 495-504 ◽  
Author(s):  
Amit Vaish ◽  
Mitchell J. Shuster ◽  
Sarawut Cheunkar ◽  
Yogesh S. Singh ◽  
Paul S. Weiss ◽  
...  
Keyword(s):  
Small Molecule ◽  
Membrane Receptors ◽  
Molecule Recognition
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