Callus induction and plant regeneration from cotyledonary explants of ash gourd (Benincasa hispida L.)

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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Callus induction from protocorm-like body segments and plant regeneration inCymbidium(Orchidaceae)

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.2004.11511781 ◽

2004 ◽

Vol 79 (3) ◽

pp. 406-410 ◽

Cited By ~ 16

Author(s):

Le Van Tuong Huan ◽

M. Tanaka

Keyword(s):

Plant Regeneration ◽

Callus Induction ◽

Body Segments

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Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)

Grassland Science ◽

10.1111/j.1744-697x.2010.00195.x ◽

2010 ◽

Vol 56 (4) ◽

pp. 198-204 ◽

Cited By ~ 2

Author(s):

Xun Wang ◽

Yoichiro Hoshino ◽

Toshihiko Yamada

Keyword(s):

Plant Regeneration ◽

Callus Induction ◽

Zoysia Japonica

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In vitro shoot regeneration through anther culture of Brassica spp.

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v35i2.5896 ◽

1970 ◽

Vol 35 (2) ◽

pp. 331-341 ◽

Cited By ~ 3

Author(s):

MA Sayem ◽

M Maniruzzaman ◽

SS Siddique ◽

M Al-Amin

Keyword(s):

Plant Regeneration ◽

Anther Culture ◽

Shoot Regeneration ◽

Callus Induction ◽

Maximum Rate ◽

Media Composition ◽

Shoot Initiation ◽

The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

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