The effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species

2010 ◽  
Vol 102 (1) ◽  
pp. 79-86 ◽  
Author(s):  
K. Głowacka ◽  
S. Jeżowski ◽  
Z. Kaczmarek
Keyword(s):  
Plant Regeneration ◽  
Developmental Stage ◽  
Callus Induction ◽  
Induction Medium

scholarly journals Efficient Plant Regeneration from Cotyledon and Midrib Derived Callus in Eggplant (Solanum melongena L.)

1970 ◽  
Vol 14 ◽  
pp. 31-38 ◽  
Author(s):  
M Rahman ◽  
M Asaduzzaman ◽  
N Nahar ◽  
MA Bari
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Key Words ◽  
Somatic Embryo ◽  
Callus Induction ◽  
Somatic Embryos ◽  
Solanum Melongena ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium

Somatic embryos were obtained from cotyledon and midrib explants of Solanum melongena L., cultivar Loda. For callus induction, medium was supplemented with different concentrations of auxin singly or in combination with BAP. The best callusing 83-85% was obtained from both of the explants cultured on MS medium containing 2.0 mgl-1NAA + 0.05 mgl-1BAP. Somatic embryogenesis and shoot regeneration was achieved after transferring the calli to MS medium supplemented with BAP, GA3, NAA and Zeatin. Cotyledon derived calli showed better performance (87%) for regeneration than that of midrib (82%) when sub cultured on MS medium having 2.0 mgl-1 Zeatin + 1.0 mgl-1 BAP. For root induction, MS + 3.0 mgl-1 IBA was proved to be better treatment for average number (14-15) and mean length (12 cm) of roots than those of other treatments. Key words: Eggplant; cotyledon; midrib; callus induction; somatic embryo J. bio-sci. 14: 1-9, 2006

scholarly journals Plant Regeneration from Protocorm-derived Callus of Five Paphiopedilum Hybrids

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1101D-1101
Author(s):  
Michael Compton
Keyword(s):  
Plant Regeneration ◽  
Growth Regulator ◽  
Callus Induction ◽  
Callus Formation ◽  
Induction Medium ◽  
Regeneration Medium ◽  
Petri Dishes ◽  
Callus Induction Medium

Callus was induced from protocorms of five Paphiopedilum hybrids (Paph. 03-1, Paph. 03-4, Paph. 03-5, Paph. 03-6, and Paph. 03-7) on callus induction medium [MS inorganics (412.5 mg NH4NO3 instead of 1650 mg and 475 mg KNO3 instead of 1900 mg) and vitamins plus (per liter) 0.1 g myo-inositol, 30 g sucrose, and 2.5 g Gelrite; pH 5.5] containing various concentrations and combinations of thidiazuron (TDZ; 4.5 and 45 μm) and 2,4-D (4.5 and 45 μm). Callus formation was greatest for protocorms of Paph. 03-1, Paph. 03-4, Paph. 03-6, and Paph. 03-7. Among the most competent hybrids, callus formation was greatest among protocorms induced in medium containing 4.5 μm 2,4-D and 4.5 to 45 μm TDZ. Induced calli were transferred to 100 × 15 mm petri dishes containing 25 mL of PLB and plant regeneration medium (similar to callus induction medium) containing various concentrations of either benzyladenine (BA; 0.5, 5, or 10 μm), TDZ (0.25, 2.5, or 5 μm) or no growth regulator (control). PLB and plant formation was greatest on medium containing BA.

The Effects of Cytokinins on Plant Regeneration of Vetiver Grass (Vetiveria nemoralis A. Camus cv. Roiet)

2015 ◽  
Vol 804 ◽  
pp. 259-262
Author(s):  
Chonnikarn Khunchuay ◽  
Kanokporn Sompornpailin
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Regeneration Medium ◽  
Shoot Number ◽  
Induction Experiment

The optimum ratios of auxin and cytokinin are necessary for callus induction and plant regeneration. This ratio is a key function involving in the promoting cell division and proliferation in tissue culture. The axillary buds of in vitro plantlets fromVetiveria nemoralisA. Camuscv. Roiet were used as explants for the callus induction experiment. These explants were cultured on Murashige & Skoog (MS) medium [1] supplemented with various combinations of auxins and cytokinins. Under this experimental study, the highest frequency of callus induction was found on MS medium supplemented with 2 mgL-1α-naphthalene acetic acid (NAA) and 1 mgL-12-furanylmethyl-1H-purine-6-amine (kinetin) (62.5%). On the other hand the combination of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (BAP) was toxicity to this explants. All culturing explants were dead and no calli appearance. The calli derived from each medium were transferred into the same regeneration medium (MS with 1 mgL-1NAA and 2 mgL-1BAP). After culturing on regeneration medium, calli induced from the highest callus induction medium have shown high frequencies of regeneration and also shoot number per callus (58.33% and 7.1 shoots).

scholarly journals Effect of Silver Nitrate and Amino Acids on High Frequency Plants Regeneration in Barley (Hordeum vulgare L.)

2015 ◽  
Vol 25 (1) ◽  
pp. 37-50 ◽  
Author(s):  
Mozidul Haque ◽  
Abu Baker Siddique ◽  
SM Shahinul Islam
Keyword(s):  
Amino Acids ◽  
Plant Regeneration ◽  
Callus Induction ◽  
High Frequency ◽  
Fold Increase ◽  
Negative Influence ◽  
Induction Medium ◽  
Hordeum Vulgare L ◽  
Regeneration Efficiency ◽  
Barley Genotypes

Three Bangladeshi barley genotypes viz. BARI barley?1, 3 and 6 were selected for this study to evaluate the efficiency of callus induction and plant regeneration. The effect of five doses of AgNO3 singly, and combined with amino acids (Lproline, L?glutamine) on callus induction and plant regeneration efficiency was evaluated using BARI barley?3 and 6. The maximum values of callus induction were recorded at 49.20 and 32.66% for BARI barley?6 and 3, respectively when 2.0 mg/l AgNO3 and 200 mg/l L?glutamine were added to the callus induction medium. Moreover, plant regeneration remarkably increased on MS + 1.0 mg/l BAP + 1.5 mg/l AgNO3 + 150 mg/l L?glutamine as 37.20% in BARI barley?6 and 16.13% in BARI barley?3. For rooting AgNO3 singly affect positively, whereas negative influence was observed in combinations with any amino acids. However, by using AgNO3 and amino acids, around < 4, < 27 and < 5 fold increase in callus induction were obtained. Regeneration and rooting were also found to increase considerably.Plant Tissue Cult. & Biotech. 25(1): 37-50, 2015 (June)

scholarly journals Kiwiberry (Actinidia Arguta) Hexaploid Plant Regeneration Through Culture of Endosperm Isolated From Fresh and Year-old Dry Seeds

2021 ◽  
Author(s):  
Mohib Abdullah ◽  
Elwira Sliwinska ◽  
Grzegorz Góralski ◽  
Piotr Latocha ◽  
Monika Tuleja ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nuclear Dna ◽  
Callus Formation ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
Plant Production ◽  
Induction Medium ◽  
Root Induction ◽  
Shoot Buds

Abstract Endosperm, an ephemeral and storage tissue, serves as a source of nutrition and protection during embryo development and germination. It can be used for the cultivation of polyploid plants in vitro. Here, a protocol of plant regeneration and acclimatization from the endosperm-derived calli of Actinidia arguta has been developed. Seeds excised from fresh fruit and dry seeds stored for one year served as the sources of endosperm explants of selected tetraploid cultivars of A. arguta. Callus Induction Medium (CIM; containing 0.25, 0.5, or 1 mg/l of TDZ) and Actinidia Endosperm Medium (AEM; containing 2 mg/l of 2,4-D and 5 mg/l of kinetin) were used to study the organogenic responses of the calli. On AEM, the source of explant did not significantly affect the rate of callus induction for any of the tested cultivars. Similarly, no organogenic events were observed. In contrast, on CIM both the source of explants and the cultivar origin caused significant differences in callus formation and subsequent organogenic events. Histological and ultrastructural analyses revealed the adventitious nature of shoot bud formation on these media. The most efficient elongation of shoot buds was achieved after transferring organogenic calli with adventitious shoot buds to a medium supplemented with zeatin or meta-topolin. Robust root induction with minimal basal callus formation occurred on the medium with indole-3-acetic acid. Flow cytometric analysis revealed that the nuclear DNA content in the leaves of some regenerants (4.5 pg/2C) was approximately 50% higher than that in the tetraploid seedlings (3.1 pg). This finding confirmed that those regenerants originated from the endosperm. The regeneration of hexaploid plants was more efficient when endosperm from fresh seeds served as an explant; therefore, fresh rather than dry seeds are recommended for endosperm-derived plant production. The hexaploid plants of A. arguta can serve as an important source of breeding material.

scholarly journals Induksi Kalus dan Regenerasi Beberapa Genotipe Gandum (Triticum aestivum L.) secara In Vitro

2016 ◽  
Vol 6 (2) ◽  
pp. 57
Author(s):  
Atmitri Sisharmini ◽  
Aniversari Apriana ◽  
Sustiprijatno Sustiprijatno
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Basal Medium ◽  
Triticum Aestivum L ◽  
Induction Medium ◽  
In Vitro Plant Regeneration ◽  
Regeneration Medium ◽  
Wheat Genotypes

<p>Callus Induction and In Vitro Plant Regeneration of<br />Wheat Genotypes (Triticum aestivum L.). Atmitri<br />Sisharmini, Aniversari Apriana, and Sustiprijatno. Development<br />of a reliable in vitro plant regeneration procedure for<br />wheat is a prerequisite for its improvement by genetic transformation.<br />The purpose of this study was to obtain methods<br />of callus induction and regeneration of wheat genotypes.<br />This experiment was conducted at ICABIOGRAD. Immature<br />embryos from four wheat genotypes, ie Perdix, Naxos Wew,<br />Combi and Fasan were used to induce callus formation and<br />regeneration rate of callus. For the preparation of callus<br />induction medium, MS-L7 basal medium was supplemented<br />with combination of growth regulators 2,4 dichlorophenoxy<br />acetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid<br />(picloram). While, plant regeneration medium was prepared<br />using MS basal medium supplemented with combination of<br />three growth regulators i.e. IAA, BAP and kinetin. The results<br />showed that genotype, in vitro culture medium and growth<br />regulators played a dominant role in callus induction and<br />plantlet regeneration. All the 4 genotypes responded positively<br />to callus induction, however, variability was observed<br />not only among the genotypes but also within callus<br />induction medium used. The best induction medium was<br />the MS-L7 basal medium supplemented with combination of<br />phytohormon 4 mg/l 2,4-D + 2 mg/l picloram (GIK-3) which<br />showed 100% callus induction frequency. Whereas, the best<br />regeneration medium was shown by MS basal medium with<br />combination of phytohormon 1.5 mg/l BAP dan 0.5 mg/l<br />kinetin (RG3). Regarding plant regeneration, Perdix was the<br />most responsive genotype to be regenerated with regeneration<br />frequency of 57.33%. The successfully acclimatized<br />planlets in greenhouse were obtained from Perdix and<br />Naxos Wew genotypes. These results will potentially facilitate<br />genetic transformation research of wheat in Indonesia.</p>

scholarly journals Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion

2015 ◽  
Vol 33 (4) ◽  
pp. 441-447 ◽  
Author(s):  
Iyyakkannu Sivanesan ◽  
Kim E Kyoung ◽  
Kim M Kyoung ◽  
Ko E Young ◽  
Se W Park
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Zygotic Embryo ◽  
Mature Embryo ◽  
Sem Analysis ◽  
Induction Medium ◽  
Ms Medium ◽  
Embryogenic Callus Induction

ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus induction. Scanning electron micrograph (SEM) analysis of embryogenic callus showed all stages of somatic embryos such as globular, scuetellar and coleoptilar. Plant regeneration was significantly affected by the composition of embryogenic callus induction medium. The greatest frequency of somatic embryo conversion was obtained from embryogenic callus developed in MS medium with 2,4-D (70.1%) followed by picloram (38.9%) and 2,4-D + picloram (34.5%). The germinated plantlets were further developed on the half-strength MS medium containing 3% sucrose and were acclimatized in the culture room with 98% survival rate.

Callus Induction and Plant Regeneration ofRosa hybrida

2014 ◽  
Vol 49 (5) ◽  
pp. 595 ◽  
Author(s):  
Feng Huan ◽  
Yi Shuli ◽  
Xie Jiaheng ◽  
Lei Mengqi ◽  
Huang Xuan
Keyword(s):  
Plant Regeneration ◽  
Callus Induction

Seedling‐derived leaf and root tip as alternative explants for callus induction and plant regeneration in maize

2021 ◽  
Author(s):  
Chenchen Wang ◽  
Haizhen Ma ◽  
Weiwei Zhu ◽  
Jiedao Zhang ◽  
Xiangyu Zhao ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Root Tip
Sign in / Sign up

Export Citation Format

Share Document