DNA metabarcoding improves the detection of multiple stressor responses of stream invertebrates to increased salinity, fine sediment deposition and reduced flow velocity

2021 ◽  
Vol 750 ◽  
pp. 141969 ◽  
Author(s):  
Arne J. Beermann ◽  
Marie-Thérése Werner ◽  
Vasco Elbrecht ◽  
Vera M.A. Zizka ◽  
Florian Leese
Keyword(s):  
Flow Velocity ◽  
Fine Sediment ◽  
Sediment Deposition ◽  
Stream Invertebrates ◽  
Multiple Stressor ◽  
Dna Metabarcoding ◽  
Reduced Flow

scholarly journals Analyzing multiple stressor effects on EPT taxa in a mesocosm experiment with DNA metabarcoding

2021 ◽  
Vol 4 ◽  
Author(s):  
Marie-Thérése Werner ◽  
Arne Beermann ◽  
Vasco Elbrecht ◽  
Vera Zizka ◽  
Florian Leese
Keyword(s):  
Water Quality ◽  
Flow Velocity ◽  
Fine Sediment ◽  
Sediment Deposition ◽  
Species Level ◽  
Taxonomic Resolution ◽  
Response Patterns ◽  
Multiple Stressor ◽  
Dna Metabarcoding ◽  
Ept Taxa

Multiple stressors diversely and often adversely affect stream ecosystems around the globe. Therefore, understanding multiple stressor effects on different organisms is essential for a better ecosystem understanding, an accurate water quality assessment and improved ecosystem management. However, while multiple stressor effects should be assessed at species level this taxonomic resolution is often not achieved e.g. for stream macroinvertebrates. Due to their high abundance and diversity, species-level identification is often not feasible with morphology‑based approaches. DNA metabarcoding represents an alternative approach for studying multiple stressor interactions at species level. In an outdoor experiment over 10,000 specimens from the insect orders Ephemeroptera, Plecoptera and Trichoptera (EPT), which are routinely used as bioindicators, and their responses to stressors were studied. In the experiment salinity, fine sediment deposition and flow velocity were manipulated in a full‑factorial design in 64 mesocosms with two microhabitats each (streambed and leaf litter), resulting in eight replicates per treatment. DNA metabarcoding revealed 122 EPT Operational Taxonomic Units (OTUs), from which the most abundant 27 alone showed 14 different response patterns to the applied stressors. The high taxonomic resolution achieved by DNA metabarcoding revealed species specific stressor responses that were hidden at a lower taxonomic resolution. As a prominent example, Rhithrogena semicolorata responded negatively to fine sediment deposition and flow velocity reduction, while Ecdyonurus torrentis (both Heptageniidae, Ephemeroptera) was insensitive to experimental manipulation, highlighting different stressor responses among species within the same family (Fig. 1, Beermann et al. 2020). Even for well-studied organisms such as EPT taxa, this study shows that DNA metabarcoding has the potential to depict response patterns at species or OTU level despite high specimen abundance. Consequently, DNA metabarcoding promises to be a rewarding method when investigating and assessing multiple stressor effects on stream water quality.

Multiple-stressor effects on stream invertebrates: a mesocosm experiment manipulating nutrients, fine sediment and flow velocity

2016 ◽  
Vol 61 (4) ◽  
pp. 362-375 ◽  
Author(s):  
Vasco Elbrecht ◽  
Arne J. Beermann ◽  
Gunnar Goessler ◽  
Janis Neumann ◽  
Ralph Tollrian ◽  
...  
Keyword(s):  
Flow Velocity ◽  
Fine Sediment ◽  
Mesocosm Experiment ◽  
Stream Invertebrates ◽  
Multiple Stressor

Multiple-stressor effects on stream macroinvertebrate communities: A mesocosm experiment manipulating salinity, fine sediment and flow velocity

2018 ◽  
Vol 610-611 ◽  
pp. 961-971 ◽  
Author(s):  
Arne J. Beermann ◽  
Vasco Elbrecht ◽  
Svenja Karnatz ◽  
Li Ma ◽  
Christoph D. Matthaei ◽  
...  
Keyword(s):  
Flow Velocity ◽  
Fine Sediment ◽  
Mesocosm Experiment ◽  
Macroinvertebrate Communities ◽  
Multiple Stressor ◽  
Stream Macroinvertebrate

Is fine sediment deposition a main driver for the composition of benthic macroinvertebrate assemblages?

2013 ◽  
Vol 24 ◽  
pp. 589-598 ◽  
Author(s):  
Marta Gerta Von Bertrab ◽  
Andreas Krein ◽  
Sonja Stendera ◽  
Frankie Thielen ◽  
Daniel Hering
Keyword(s):  
Fine Sediment ◽  
Sediment Deposition ◽  
Benthic Macroinvertebrate ◽  
Macroinvertebrate Assemblages ◽  
Main Driver

scholarly journals Fine sediment deposition affects biodiversity and density of benthic macroinvertebrates: A case study in the freshwater pearl mussel river Waldaist (Upper Austria)

Limnologica ◽  
2015 ◽  
Vol 50 ◽  
pp. 54-57 ◽  
Author(s):  
P. Leitner ◽  
C. Hauer ◽  
T. Ofenböck ◽  
F. Pletterbauer ◽  
A. Schmidt-Kloiber ◽  
...  
Keyword(s):  
Benthic Macroinvertebrates ◽  
Fine Sediment ◽  
Sediment Deposition ◽  
Pearl Mussel ◽  
Freshwater Pearl Mussel ◽  
Upper Austria

Subsidy-stress and multiple-stressor effects along gradients of deposited fine sediment and dissolved nutrients in a regional set of streams and rivers

2011 ◽  
Vol 56 (9) ◽  
pp. 1916-1936 ◽  
Author(s):  
ANNIKA WAGENHOFF ◽  
COLIN R. TOWNSEND ◽  
NGAIRE PHILLIPS ◽  
CHRISTOPH D. MATTHAEI
Keyword(s):  
Fine Sediment ◽  
Dissolved Nutrients ◽  
Streams And Rivers ◽  
Multiple Stressor
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