A multi-species indirect ELISA for detection group-specific antibodies against VP7 protein of bluetongue virus

The core of BTV is organized into three concentric structures of which VP7 protein forms the major core protein. The subcore consists of VP3 protein and the innermost part of the core is made of three minor proteins: VP1, VP4, and VP6. Earlier it was reported that core-like particles (CLPs) composed of viral VP7 and VP3 proteins were produced in order to study role of VP7 protein in intermolecular interactions in the BTV assembly process. Site specific mutational studies revealed that substitution of the single lysine residue of VP7 (Lys-255) by leucine abrogated CLP formation, indicating a critical role for this lysine. In the present study, homology modeling, mutagenesis, and docking studies were carried out in order to design potent leads in modulation of VP7 protein in abrogating CLP formation.

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Synthesis of Bluetongue Virus Chimeric VP3 Molecules and Their Interactions with VP7 Protein to Assemble into Virus Core-like Particles

Virology ◽

10.1006/viro.1995.0070 ◽

1995 ◽

Vol 214 (2) ◽

pp. 593-601 ◽

Cited By ~ 7

Author(s):

SEIICHI TANAKA ◽

MICHAEL MIKHAILOV ◽

POLLY ROY

Keyword(s):

Bluetongue Virus ◽

Virus Core ◽

Vp7 Protein

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Inter-laboratory evaluation of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies

Journal of Virological Methods ◽

10.1016/j.jviromet.2015.12.001 ◽

2016 ◽

Vol 228 ◽

pp. 140-150 ◽

Cited By ~ 2

Author(s):

Jean-Baptiste Hanon ◽

Valerie Vandenberge ◽

Matthias Deruelle ◽

Ilse De Leeuw ◽

Kris De Clercq ◽

...

Keyword(s):

Bluetongue Virus ◽

Lateral Flow ◽

Laboratory Evaluation ◽

Performance Parameters ◽

Test Device ◽

Specific Antibodies ◽

Flow Test ◽

Lateral Flow Test

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Immunogenicity of Streptococcus equi subsp. equi recombinant SeM protein and bacterin in mice

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-6910 ◽

2021 ◽

Vol 41 ◽

Author(s):

Matheus C. Rosa ◽

Neida Lucia Conrad ◽

Carina M. Moraes ◽

Fábio P.L. Leite

Keyword(s):

Indirect Elisa ◽

Vaccine Development ◽

Control Group ◽

High Morbidity ◽

Specific Antibodies ◽

Whole Cells ◽

Immunodominant Antigen ◽

Promising Target ◽

Streptococcus Equi ◽

Rapid Spread

ABSTRACT: The infection caused by Streptococcus equi, known as strangles, affects the respiratory system of horses, causing high morbidity and rapid spread among the herd. Bacterin vaccines, composed of inactivated whole cells of S. equi, have variable efficacy and duration. Infected animals produce specific antibodies against SeM, the immunodominant antigen of S. equi. This makes it a promising target for vaccine development. In this context, the objective of this work was to evaluate a vaccine combining S. equi bacterin and recombinant SeM protein. Mice were vaccinated with bacterin (S. equi ~1.2 × 108CFU/ml); rSeM protein (20μg); bacterin-rSeM combination; or PBS (Control Group) and challenged with a suspension of S. equi, containing 10 × LD50. All vaccinated mice survived the challenge and produced anti-rSeM and anti-S. equi antibodies, which were assessed by indirect ELISA. The Control Group reached endpoint criteria 96 h after infection. These results demonstrate that a vaccine combining the S. equi bacterin with rSeM protein protects mice against strangles. This combination vaccine could potentially protect horses and overcome the limitations of currently available strangle vaccines.

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