A fluorescence polarization assay for nucleic acid based on the amplification of hybridization chain reaction and nanoparticles

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A hybridization chain reaction (HCR) lightened by DNA-stabilized silver nanoclusters (AgNCs) as a label-free and turn on fluorescence platform for nucleic acid assays.


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Hybridization chain reaction is a very popular isothermal nucleic acid amplification technology. A single-stranded DNA initiator triggers an alternate hybridization event between two hairpins forming a double helix polymer. Due...


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