scholarly journals Sequence-specific electrochemical detection of enzymatic amplification products of Salmonella genome on ITO electrodes improves pathogen detection to the single copy level

2018 ◽  
Vol 268 ◽  
pp. 438-445 ◽  
Author(s):  
Susana Barreda-García ◽  
Rebeca Miranda-Castro ◽  
Noemí de-los-Santos-Álvarez ◽  
María Jesús Lobo-Castañón
Keyword(s):  
Electrochemical Detection ◽  
Pathogen Detection ◽  
Single Copy ◽  
Enzymatic Amplification ◽  
Salmonella Genome

High-sensitive electrochemical detection of point mutation based on polymerization-induced enzymatic amplification

2011 ◽  
Vol 26 (7) ◽  
pp. 3187-3191 ◽  
Author(s):  
Kejun Feng ◽  
Jingjin Zhao ◽  
Zai-Sheng Wu ◽  
Jianhui Jiang ◽  
Guoli Shen ◽  
...  
Keyword(s):  
Point Mutation ◽  
Electrochemical Detection ◽  
Enzymatic Amplification

scholarly journals Proof of Concept for Recombinant Cellular Controls in Quantitative Molecular Pathogen Detection

2011 ◽  
Vol 77 (7) ◽  
pp. 2531-2533 ◽  
Author(s):  
Peter Rossmanith ◽  
Patrick Mester ◽  
Karin Frühwirth ◽  
Sabine Fuchs ◽  
Martin Wagner
Keyword(s):  
Listeria Monocytogenes ◽  
Sample Preparation ◽  
Quantitative Pcr ◽  
Pathogen Detection ◽  
Single Copy ◽  
Deletion Strain ◽  
Proof Of Concept ◽  
Targeted Deletion ◽  
Process Controls

ABSTRACTIn this study, we present the concept of internal sample process controls (ISPCs) to monitor the efficiency of an analytical chain using sample preparation and quantitative PCR (qPCR). A recombinantListeria monocytogenesΔprfA(targeted deletion) strain containing a competitive artificial single-copy genomic target was applied to naturally contaminated samples to demonstrate its analytical suitability as an ISPC.

Microfabricated Disposable DNA Sensors Based on Enzymatic Amplification Electrochemical Detection

2001 ◽  
Vol 13 (10) ◽  
pp. 882-887 ◽  
Author(s):  
Danke Xu ◽  
Kai Huang ◽  
Zhihong Liu ◽  
Yaoqing Liu ◽  
Liren Ma
Keyword(s):  
Electrochemical Detection ◽  
Dna Sensors ◽  
Enzymatic Amplification

Electrochemical detection and discrimination of single copy gene target DNA in non-amplified genomic DNA

2011 ◽  
Vol 56 (5) ◽  
pp. 1925-1931 ◽  
Author(s):  
Esmaeel Alipour ◽  
Mohammad Hossein Pournaghi-Azar ◽  
Mojtaba Parvizi ◽  
Sayed Mahdi Golabi ◽  
Mohammad Saeid Hejazi
Keyword(s):  
Electrochemical Detection ◽  
Genomic Dna ◽  
Single Copy ◽  
Single Copy Gene ◽  
Gene Target ◽  
Target Dna ◽  
Copy Gene
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