An Efficient Biosensor Based on the Synergistic Catalysis of Helicobacter pylori Urease B Subunit and Nanoplatinum for Urease Inhibitors Screening and Antagonistic Mechanism Analyzing

2021 ◽  
pp. 131284
Author(s):  
Sa Xiao ◽  
Keshuai Shang ◽  
Wenjuan Li ◽  
Xia Wang
Keyword(s):  
Helicobacter Pylori ◽  
Urease Inhibitors ◽  
Synergistic Catalysis ◽  
B Subunit ◽  
Urease B Subunit

Identification of H-2d restricted Th epitopes in Urease B subunit of Helicobacter pylori

Vaccine ◽  
2007 ◽  
Vol 25 (14) ◽  
pp. 2583-2590 ◽  
Author(s):  
Yun Shi ◽  
Chao Wu ◽  
Wei-Ying Zhou ◽  
Xu-Hu Mao ◽  
Gang Guo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
B Subunit ◽  
Urease B Subunit

Oral immunization with Helicobacter pylori urease B subunit as a treatment against Helicobacter infection in mice

1995 ◽  
Vol 109 (1) ◽  
pp. 115-121 ◽  
Author(s):  
Irène Corthésy-Theulaz ◽  
Nadine Porta ◽  
Micheline Glauser ◽  
Emilia Saraga ◽  
Anne-Catherine Vaney ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Immunization ◽  
B Subunit ◽  
Urease B Subunit

Identification of B-cell epitopes in urease B subunit of Helicobacter pylori bound by neutralizing antibodies

Vaccine ◽  
2010 ◽  
Vol 28 (32) ◽  
pp. 5220-5227 ◽  
Author(s):  
Yan Qiu ◽  
Yan-Chun Wang ◽  
Hao-Xia Tao ◽  
De-Wen Zhan ◽  
Sheng-Ling Yuan ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
B Cell ◽  
Neutralizing Antibodies ◽  
B Cell Epitopes ◽  
B Subunit ◽  
Urease B Subunit

scholarly journals The Helicobacter pylori Urease B Subunit Binds to CD74 on Gastric Epithelial Cells and Induces NF-κB Activation and Interleukin-8 Production

2006 ◽  
Vol 74 (2) ◽  
pp. 1148-1155 ◽  
Author(s):  
Ellen J. Beswick ◽  
Irina V. Pinchuk ◽  
Kyle Minch ◽  
Giovanni Suarez ◽  
Johanna C. Sierra ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Fibroblast Cell ◽  
Surface Proteins ◽  
Interleukin 8 ◽  
Gastric Epithelial Cells ◽  
Bacterial Surface ◽  
B Subunit ◽  
H Pylori ◽  
Urease B Subunit

ABSTRACT The pathogenesis associated with Helicobacter pylori infection is the result of both bacterial factors and the host response. We have previously shown that H. pylori binds to CD74 on gastric epithelial cells. In this study, we sought to identify the bacterial protein responsible for this interaction. H. pylori urease from a pool of bacterial surface proteins was found to coprecipitate with CD74. To determine how urease binds to CD74, we used recombinant urease A and B subunits. Recombinant urease B was found to bind directly to CD74 in immunoprecipitation and flow cytometry studies. By utilizing both recombinant urease subunits and urease B knockout bacteria, the urease B-CD74 interaction was shown to induce NF-κB activation and interleukin-8 (IL-8) production. This response was decreased by blocking CD74 with monoclonal antibodies. Further confirmation of the interaction of urease B with CD74 was obtained using a fibroblast cell line transfected with CD74 that also responded with NF-κB activation and IL-8 production. The binding of the H. pylori urease B subunit to CD74 expressed on gastric epithelial cells presents a novel insight into a previously unrecognized H. pylori interaction that may contribute to the proinflammatory immune response seen during infection.

scholarly journals Identification of novel bacterial urease inhibitors through molecular shape and structure based virtual screening approaches

RSC Advances ◽  
2020 ◽  
Vol 10 (27) ◽  
pp. 16061-16070
Author(s):  
Muhammad Imran ◽  
Saba Waqar ◽  
Koji Ogata ◽  
Mahmood Ahmed ◽  
Zobia Noreen ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Virtual Screening ◽  
Acid Resistance ◽  
Molecular Shape ◽  
Urease Inhibitors ◽  
H Pylori ◽  
Screening Approaches

The enzyme urease is an essential colonizing factor of the notorious carcinogenic pathogen Helicobacter pylori (H. pylori), conferring acid resistance to the bacterium.

Marked Reduction of Helicobacter pylori-Induced Gastritis by Urease Inhibitors, Acetohydroxamic Acid and Flurofamide, in Mongolian Gerbils

2001 ◽  
Vol 285 (3) ◽  
pp. 728-733 ◽  
Author(s):  
Toshihisa Ohta ◽  
Hideyuki Shibata ◽  
Toshihiko Kawamori ◽  
Masaki Iimuro ◽  
Takashi Sugimura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Marked Reduction ◽  
Acetohydroxamic Acid ◽  
Urease Inhibitors ◽  
Mongolian Gerbils
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