Immunological features and the ability of inhibitory effects on enzymatic activity of an epitope vaccine composed of cholera toxin B subunit and B cell epitope from Helicobacter pylori urease A subunit

2011 ◽  
Vol 93 (5) ◽  
pp. 1937-1945 ◽  
Author(s):  
Le Guo ◽  
Xiaokang Li ◽  
Feng Tang ◽  
Yunmian He ◽  
Yingying Xing ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Cell Epitope ◽  
Cholera Toxin B Subunit ◽  
Inhibitory Effects ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
B Cell Epitope ◽  
A Subunit ◽  
Urease A Subunit

Oral Vaccination Against Helicobacter pylori with Recombinant Cholera Toxin B-Subunit

Helicobacter ◽  
2005 ◽  
Vol 10 (4) ◽  
pp. 345-352 ◽  
Author(s):  
Eiji Kubota ◽  
Takashi Joh ◽  
Satoshi Tanida ◽  
Makoto Sasaki ◽  
Hiromi Kataoka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Cholera Toxin ◽  
Cholera Toxin B Subunit ◽  
Oral Vaccination ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit

scholarly journals Spray-Dried Formulation of Epicertin, a Recombinant Cholera Toxin B Subunit Variant That Induces Mucosal Healing

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 576
Author(s):  
Micaela A. Reeves ◽  
Joshua M. Royal ◽  
David A. Morris ◽  
Jessica M. Jurkiewicz ◽  
Nobuyuki Matoba ◽  
...  
Keyword(s):  
Gastric Acid ◽  
Cholera Toxin ◽  
Oral Formulation ◽  
Size Exclusion ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Enteric Coated ◽  
Spray Dried

Epicertin (EPT) is a recombinant variant of the cholera toxin B subunit, modified with a C-terminal KDEL endoplasmic reticulum retention motif. EPT has therapeutic potential for ulcerative colitis treatment. Previously, orally administered EPT demonstrated colon epithelial repair activity in dextran sodium sulfate (DSS)-induced acute and chronic colitis in mice. However, the oral dosing requires cumbersome pretreatment with sodium bicarbonate to conserve the acid-labile drug substance while transit through the stomach, hampering its facile application in chronic disease treatment. Here, we developed a solid oral formulation of EPT that circumvents degradation in gastric acid. EPT was spray-dried and packed into enteric-coated capsules to allow for pH-dependent release in the colon. A GM1-capture KDEL-detection ELISA and size-exclusion HPLC indicated that EPT powder maintains activity and structural stability for up to 9 months. Capsule disintegration tests showed that EPT remained encapsulated at pH 1 but was released over 180 min at pH 6.8, the approximate pH of the proximal colon. An acute DSS colitis study confirmed the therapeutic efficacy of encapsulated EPT in C57BL/6 mice upon oral administration without gastric acid neutralization pretreatment compared to vehicle-treated mice (p < 0.05). These results provide a foundation for an enteric-coated oral formulation of spray-dried EPT.

Cholera toxin B subunit promotes the induction of regulatory T cells by preventing human dendritic cell maturation

2008 ◽  
Vol 84 (3) ◽  
pp. 661-668 ◽  
Author(s):  
Antonella D'Ambrosio ◽  
Manuela Colucci ◽  
Orsola Pugliese ◽  
Francesca Quintieri ◽  
Monica Boirivant
Keyword(s):  
T Cells ◽  
Dendritic Cell ◽  
Regulatory T Cells ◽  
Dendritic Cell Maturation ◽  
Cell Maturation ◽  
Cholera Toxin B Subunit ◽  
Human Dendritic Cell ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit
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