In conventional earthquake resistance design approach (the ductility-design philosophy), the energy dissipation mechanism is based on plastic deformations at scattered locations in the structure. However, this can produce permanent damage to the joints as well as the larger interstory displacements. In recently years, the base isolation technology has been adopted as a feasible and attractive way in improving seismic resistance of structures. It can shift the natural periods of structures away from the rich periods contents of earthquake motions, but also provide considerable supplemental damping to dissipate seismic energy transmitted into structures during earthquakes. In this paper, uniaxial, biaxial, and triaxial shaking table tests are performed to study the seismic behavior of a 0.4-scale three-story isolated steel structure in the National Center for Research on Earthquake Engineering in Taiwan. Experimental results demonstrate that structures with hybrid rubber bearings can actually decrease the seismic responses of the superstructure. It has been proved through the shaking table tests that the rubber bearing is a very promising tool to enhance the seismic resistibility of structures. Moreover, it is illustrated that the proposed analytical model and finite element formulation in this paper can well predict the mechanical behavior of rubber bearings and seismic responses of the base-isolated structures.