Lipid profile of extracellular vesicles and their relationship with bovine oocyte developmental competence: New players in intra follicular cell communication

2021 ◽  
Author(s):  
Juliano Coelho da Silveira ◽  
Gabriella Mamede Andrade ◽  
Rosineide Costa Simas ◽  
Helio Alves Martins-Júnior ◽  
Marcos Nogueira Eberlin ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Extracellular Vesicles ◽  
Cell Communication ◽  
Developmental Competence ◽  
Follicular Cell ◽  
Bovine Oocyte ◽  
Oocyte Developmental Competence

327 OOCYTE-SECRETED FACTORS DIRECTLY AFFECT OOCYTE DEVELOPMENTAL COMPETENCE DURING IN VITRO MATURATION OF THE BOVINE CUMULUS - OOCYTE COMPLEX

2006 ◽  
Vol 18 (2) ◽  
pp. 271 ◽  
Author(s):  
T. S. Hussein ◽  
R. B. Gilchrist ◽  
J. G. Thompson
Keyword(s):  
In Vitro Maturation ◽  
Cumulus Cell ◽  
Blastocyst Stage ◽  
Developmental Competence ◽  
Bovine Oocyte ◽  
Paracrine Factors ◽  
Cell Functions ◽  
Oocyte Developmental Competence ◽  
Secreted Factors

Paracrine factors secreted by the oocyte (oocyte-secreted factors, OSFs) regulate a broad range of cumulus cell functions including proliferation, differentiation, and apoptosis. The capacity of oocytes to regulate their own microenvironment by OSFs may in turn contribute to oocyte developmental competence. The aim of this study was to determine if OSFs have a direct influence on bovine oocyte developmental competence during in vitro maturation (IVM). Cumulus-oocyte complexes (COCs) were obtained by aspiration of >3-mm follicles from abattoir-derived ovaries. IVM was conducted in Bovine VitroMat (Cook Australia, Eight Mile Plains, Brisbane, Australia) supplemented with 0.1 IU/mL rhFSH for 24 h under 6% CO2 in air at 38.5�C. In the first experiment, COCs were co-cultured with denuded oocytes (DOs, 5/COC in 10 �L) beginning at either 0 or 9-h of IVM. To generate the 9-h DO group, COCs were first cultured intact for 9-h and then denuded. In the second experiment, specific OSFs, recombinant bone morphogenetic protein-15 (BMP-15) and growth differentiation factor 9 (GDF-9), were prepared as partially purified supernatants of transfected 293H cells, and used as 10% v/v supplements in Bovine VitroMat. Treatments were: (1) control (no supplement), (2) BMP-15, (3) GDF-9, (4) BMP-15 and GDF-9, and (5) untransfected 293H control. Following maturation, in vitro production of embryos was performed using the Bovine Vitro system (Cook Australia) and blastocysts were examined on Day 8 for development. Developmental data were arcsine-transformed and analyzed by ANOVA, followed by Tukey's test. Cell numbers were analyzed by ANOVA. Co-culturing intact COCs with DOs from 0 or 9 h did not affect cleavage rate, but increased (P < 0.001) the proportion of cleaved embryos that reached the blastocyst stage post-insemination (50.6 � 1.9 and 61.3 � 1.9%, respectively), compared to COCs cultured alone (40.7 � 1.4%). Therefore, paracrine factors secreted by DOs increased the developmental competence of oocytes matured as COCs. OSFs also improved embryo quality, as co-culture of COCs with DOs (0 or 9 h) significantly increased total cell (156.1 � 1.3 and 159.1 � 1.3, respectively) and trophectoderm (105.7 � 1.3 and 109.8 � 0.4, respectively) numbers, compared to control COCs (total = 148 � 1.2, trophectoderm = 98.2 � 0.8, P < 0.001). BMP-15 alone or with GDF-9 also significantly (P < 0.001) increased the proportion of oocytes that reached the blastocyst stage post insemination (57.5 � 2.4% and 55.1 � 4.5%, respectively), compared to control (41.0 � 0.9%) and 293H-treated (27.1 � 3.1%) COCs. GDF-9 also increased blastocyst yield (49.5 � 3.9%) but not significantly. These results are the first to demonstrate that OSFs, and particularly BMP-15 and GDF-9, directly affect bovine oocyte developmental competence. These results have far-reaching implications for improving the efficiency of IVM in domestic species and human infertility treatment, and support the role of OSF production by oocytes as a diagnostic marker for developmental competence.

scholarly journals Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e78505 ◽  
Author(s):  
Md. Mahmodul Hasan Sohel ◽  
Michael Hoelker ◽  
Sina Seifi Noferesti ◽  
Dessie Salilew-Wondim ◽  
Ernst Tholen ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Developmental Competence ◽  
Bovine Oocyte ◽  
Oocyte Developmental Competence

The Effect of Granulosa Cells Apoptosis on the Cumulus Expansion and the Developmental Competence of Bovine Oocytes

2014 ◽  
Vol 997 ◽  
pp. 251-254 ◽  
Author(s):  
Wei Guo Feng ◽  
Zhi Fang Pan
Keyword(s):  
Granulosa Cells ◽  
Culture System ◽  
Developmental Competence ◽  
Bovine Oocyte ◽  
Cumulus Expansion ◽  
Oocyte Developmental Competence ◽  
Bovine Oocytes

In this study, we used the well in the well (WIW) culture system to study the effect of granulosa cells apoptosis on the cumulus expansion and the developmental competence of bovine oocytes. Results: The apoptosis of granulosa cells affect the cumulus expansion of bovine oocytes significantly. Especially when the percentage of granulosa cells apoptosis exceed 40%, the cumulus expansion was worse. The cumulus expansion affect the oocyte developmental competence of bovine oocytes significantly. The developmental competence of bovine oocyte increases with the increasing of cumulus expansion.

scholarly journals Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress

2009 ◽  
Vol 26 (11-12) ◽  
pp. 605-611 ◽  
Author(s):  
Mahdi Zhandi ◽  
Armin Towhidi ◽  
Mohammad Hossein Nasr-Esfahani ◽  
Poopak Eftekhari-Yazdi ◽  
Ahmad Zare-Shahneh
Keyword(s):  
Heat Stress ◽  
Growth Factor ◽  
Detrimental Effect ◽  
Developmental Competence ◽  
Insulin Like Growth Factor ◽  
Bovine Oocyte ◽  
Oocyte Developmental Competence

scholarly journals The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality

2015 ◽  
Vol 30 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Sara D.M. Valckx ◽  
Jessie De Bie ◽  
Ellen D. Michiels ◽  
Ilse G. Goovaerts ◽  
Usha Punjabi ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Embryo Quality ◽  
Developmental Competence ◽  
Bovine Oocyte ◽  
Human Follicular Fluid ◽  
Oocyte Developmental Competence
Sign in / Sign up

Export Citation Format

Share Document