Polypropylene mesh seeded with fibroblasts: A new approach for the repair of abdominal wall defects in rats

2017 ◽  
Vol 49 (3) ◽  
pp. 383-392 ◽  
Author(s):  
A. Mohsina ◽  
Naveen Kumar ◽  
A.K. Sharma ◽  
Sameer Shrivastava ◽  
Dayamon D. Mathew ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Abdominal Wall Defects ◽  
New Approach

How safe is the use of prosthetic materials in the repair of abdominal-wall defects in malnourished subjects?

Open Medicine ◽  
2009 ◽  
Vol 4 (3) ◽  
pp. 331-336 ◽  
Author(s):  
Mehmet Karahan ◽  
Hakan Kulacoglu ◽  
Duray Seker ◽  
Zafer Ergul ◽  
Aysel Kiziltay ◽  
...  
Keyword(s):  
Wound Healing ◽  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Histopathological Examination ◽  
Abdominal Wall Defect ◽  
Infectious Complications ◽  
Abdominal Wall Defects ◽  
Tissue Samples ◽  
Significant Difference ◽  
Histopathological Studies

AbstractIncisional hernias and abdominal-wall defects consume large amounts of healthcare resources. Use of mesh is effective in treatment of these disorders and can decrease the rate of recurrence. This experimental study focused on the safety of mesh use in the setting of malnutrition, a condition that impairs wound healing. Rats were divided into two groups: normally fed and food-restricted. An abdominal-wall defect, 2 by 2 cm, was covered with polypropylene mesh, 2.5 by 2.5 cm. After sacrifice of the rats at the 21st and 60th days, tissue samples were sent for tensiometric and histopathological studies. No significant difference in infectious complications was observed between the two groups. Tensiometry revealed no significant differences between the groups. On histopathological examination, the only difference noted was in the vascularization scores of normally fed rats. For malnourished subjects that survived after surgery, the use of polypropylene mesh appeared safe in the closure of abdominal-wall defects, with no increase in infection rate and satisfactory wound healing.

Collagen foil (TissuFoil E) reduces the formation of adhesions when using polypropylene mesh for the repair of experimental abdominal wall defects

2006 ◽  
Vol 21 (8) ◽  
pp. 840-846 ◽  
Author(s):  
Frank Schönleben ◽  
Thomas Reck ◽  
Andrea Tannapfel ◽  
Werner Hohenberger ◽  
Ignaz Schneider
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Abdominal Wall Defects

scholarly journals Comparative analysis of autodermal graft and polypropylene mesh use in large incisional hernia defects reconstruction

2013 ◽  
Vol 70 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Danilo Stojiljkovic ◽  
Predrag Kovacevic ◽  
Milan Visnjic ◽  
Irena Jankovic ◽  
Goran Stevanovic ◽  
...  
Keyword(s):  
Incisional Hernia ◽  
Hospital Stay ◽  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Synthetic Mesh ◽  
Abdominal Wall Defects ◽  
Tension Free ◽  
Large Incisional Hernia ◽  
Significant Difference ◽  
Duration Of Surgery

Background. Large defects of the abdominal wall caused by incisional hernia still represent a challenging problem in plastic, reconstructive, and abdominal surgery. For their successful tension-free repair a proper selection of reconstructive material is essential. In the last decades, the use of synthetic meshes was dominant while biological autodermal grafts were rarely used. The aim of the study was to comparatively analyse efficacy and safety of autodermal graft and polypropylene mesh in surgical treatment of large abdominal wall defects. Methods. This prospective comparative clinical study enroled 40 patients surgically treated for large incisional hernia repair in a 10-year period. The patients were divided into two equal groups consisting of 20 subjects and treated either by biological autodermal graft or by synthetic polypropylene mesh. The surgical techniques of reconstruction, duration of surgery, the occurrence of early, minor, and major (severe) and delyed complications and hospital stay were analyzed. The average follow-up took 2 years. Results. Statistically significant differences in demographic characteristics of patients and in size of defects were not found. The surgical technique of reconstruction with an autodermal graft was more complicated. The duration of surgery in patients treated with autodermal grafts was significantly longer. There was no statistically significant difference regarding occurrence of early, minor postoperative complications and hospital stay in our study. Two severe complications were registered in the synthetic mesh group: intestinal obstruction and enterocutaneous fistula. The recurrence rate was 10% in the autodermal graft group and 15% in the group with a synthetic mesh. Conclusion. Tension-free repair of large incisional hernia with autodermal grafts was unjustly neglected despite the fact that it is safe and effective. It can be applied in all cases where synthetic mesh are not indicated (presence of infection, immunodeficient patients, after radiotherapy). They are especially important in war surgery and in lack of funds when commercial grafts cannot be purchased.

scholarly journals Expanded polytetrafluoroethylene and polypropylene in the repairing of abdominal wall defects in Wistar rats: comparative study

2006 ◽  
Vol 21 (6) ◽  
pp. 409-415 ◽  
Author(s):  
Armando José d'Acampora ◽  
Fabrícia Slomski Joli ◽  
Ricardo Tramonte
Keyword(s):  
Connective Tissue ◽  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Wistar Rats ◽  
Fibrous Tissue ◽  
Microscopic Analysis ◽  
Surrounding Tissue ◽  
Control Group ◽  
Abdominal Wall Defects ◽  
Surgical Wound

PURPOSE: To compare the use of polypropylene and PTFE meshes in the treatment of incisional hernias made experimentally on Wistar rats. METHODS: The experiment used 24 Wistar rats divided into three cohorts: C-A (ressection of a segment of abdominal wall), E-A (ressection and placement of PTFEe mesh), E-B (resection and placement of polypropylene mesh). After 28 days, the mesh and the surrounding tissue were removed and submitted to macroscopic analysis (assessment of the abdominal wall for presence of abscess in the surgical wound and/or skin necrosis, and adhesions) as well as microscopic analysis (presence of fibrosis, necrosis and abscess, counting of macrophages, mononuclears and polymorphonuclears). RESULTS: Adhesions and abscesses in the surgical wound were observed more commonly associated to the group treated with PTFEe. The size of the fibrous tissue was greater in the group treated with polypropylene. Cohort E-A showed PTFEe mesh enveloped by organized fine connective tissue. No groups presented necrosis on the site of the insertion. The highest mononuclear inflammatory reaction occurred in association to PTFEe when compared to the control group, but the findings for the polypropylene mesh were also significant when compared to the control group. In the analysis of the results obtained for cohorts E-A and E-B, a minimal occurrence of polymorphonuclears was noticed in both groups, which indicates low tissue reactivity to both materials used in the present experiment. CONCLUSION: Even with epithelization and proliferation of connective tissue, anchorage between PTFEe and abdominal wall is insufficient, which can result in reherniations.

scholarly journals Nonwoven polypropylene prosthesis in large abdominal wall defects in rats

2012 ◽  
Vol 27 (10) ◽  
pp. 671-680 ◽  
Author(s):  
Patrick dos Santos Barros dos Reis ◽  
Vera Lucia Antunes Chagas ◽  
Jéssica Marquet Silva ◽  
Paulo Cesar Silva ◽  
Nelson Jamel ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Abdominal Wall Defect ◽  
Therapeutic Option ◽  
Abdominal Wall Defects ◽  
Scanning Calorimetry ◽  
Wall Defect ◽  
Simulation Group ◽  
Hematoxylin Eosin ◽  
Group 1

PURPOSE: To evaluate, in large abdominal wall defects surgically shaped in rats, if a synthetic polypropylene nonwoven prosthesis could be used as a therapeutic option to conventional polypropylene mesh. METHODS: Twenty four (24) Wistar rats were enrolled into three groups. Group 1 (Simulation group) with an abdominal wall defect of 3 X 3 left untreated and Groups 2 and 3, respectively treated with a conventional polypropylene mesh and a polypropylene nonwoven (NWV) prosthesis to cover the breach. At the 45th postoperatively day, adhesion (area and strength) and vascularization of Groups 2 and 3 were evaluated. The histological preparations with Hematoxylin-Eosin, Tricromium of Masson, Pricrosirius red and polarization with birefringence, and also the structural analysis of the prostheses carried on by Thermogravimetry and Differential Scanning Calorimetry were also assessed. RESULTS: There were no significant differences between the Groups 2 and 3. CONCLUSION: In rats, the polypropylene nonwoven prosthesis showed to be safe and has to be considered as an alternative to conventional mesh manufactured by weaving in the treatment of great defects of the abdominal wall.

scholarly journals Plasma Derived Products for Polypropylene Mesh Integration in Abdominal Wall Defects: Procedure Description and Partial Results

Chirurgia ◽  
2021 ◽  
Vol 116 (5) ◽  
pp. 599
Author(s):  
Valentin Popescu ◽  
Traian Patrascu ◽  
Dan Andras ◽  
Marius-Septimiu Petrutescu ◽  
Sergiu Cecoltan ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Abdominal Wall Defects

Platelet Rich Fibrin Augmented Versus Non-Augmented Glycerolized Bovine Pericardium and Polypropylene Mesh for Repairing of Large Abdominal Wall Defects

2020 ◽  
Vol 3 (2) ◽  
pp. 7
Author(s):  
M. H. El-Husseiny ◽  
El-Maghraby H. M ◽  
Alakraa A. M
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Autologous Blood ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Bovine Pericardium ◽  
Abdominal Wall Defects ◽  
Gray Scale ◽  
Platelet Rich Fibrin ◽  
Post Implantation

This study aimed to evaluate the use of glycerolized bovine pericardium (GBP) compared to polypropylene mesh (PPM) in repairing of large abdominal wall defects in animal model, and to investigate the role of platelet rich fibrin (PRF) in promoting this repair. Fresh bovine pericardium collected from local abattoir were processed and preserved in 99.5 percent glycerol. PRF matrix was harvested from fresh autologous blood (10 ml) after centrifugation. Full-thickness, mid-ventral abdominal wall defects (6 × 10 cm) were surgically created in 36 healthy goats (9 goats/group) and were repaired with an equal size of GBP, PPM, GBP-PRF, or PPM-PRF. Qualitative and gray scale quantitative ultrasonography were adopted at day 1, 1, 2, 3, 4, 8 and 12 weeks post-implantation. Three goats per group were slaughtered at 4, 8 and 12 weeks post-implantation for further gross, histopathological and tensiometric (tensile strength, load at failure and strain percent) evaluations. Ultrasonography revealed significant (P˂ 0.05) improvement of implant gray scale, low subcutaneous edema and reduction of skin implant distance in PRF-augmented groups. Besides, a substantial improvement of connective tissue covering, implant incorporation, new blood vessels formation, and reduction of the inflammatory cells infiltrations were observed. Tensiometric parameters were improved in GBP-PRF group compared to the other groups. In conclusion, the obtained results not only proved the superiority of GBP over PPM, but also the advantage of PRF-augmented over non-augmented implants in treatment of large abdominal wall defects. Ultrasonographic analysis provided a satisfactory tool to evaluate the healing process of the abdominal wall defects.

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