histopathological studies
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Author(s):  
Lilian de Paula Gonçalves Reis ◽  
Antonio Jesús Lora-Benítez ◽  
Ana Mª Molina-López ◽  
Rafael Mora-Medina ◽  
Nahúm Ayala-Soldado ◽  
...  

Bisphenol A (BPA) is a chemical substance commonly used in the manufacture of plastic products. Its inhalation or ingestion from particles in suspension, water, and/or polluted foods can trigger toxic effects related to endocrine disruption, resulting in hormonal, reproduction, and immunological alterations in humans and animals. The zebrafish (Danio rerio) is an ideal experimental model frequently used in toxicity studies. In order to assess the toxic effects of BPA on reproduction and embryonic development in one generation after parental exposure to it, a total of 80 zebrafish, males and females, divided into four groups in duplicate (n = 20) were exposed to BPA concentrations of 500, 50, and 5 µg L−1, along with a control group. The fish were kept in reproduction aquariums for 21 days. The embryos obtained in the crosses were incubated in a BPA-free medium and observed for signs of embryotoxicity. A histopathological study (under optical and electron microscopes) was performed of adult fish gonads. The embryos of reproducers exposed to BPA were those most frequently presenting signs of embryotoxicity, such as mortality and cardiac and musculoskeletal malformations. In the histopathological studies of adult individuals, alterations were found in ovocyte maturation and in spermatazoid formation in the groups exposed to the chemical. Those alterations were directly related to BPA action, affecting fertility in both sexes, as well as the viability of their offspring, proportionally to the BPA levels to which they were exposed, so that our results provide more information by associating toxic effects on the offspring and on the next generation.


2022 ◽  
Vol 12 (2) ◽  
pp. 76-81
Author(s):  
Rafiul Karim Khan ◽  
Md Mustafizur Rahman ◽  
Nadim Ahmed ◽  
Rajib Dey Sarker

Background: Undoubtedly the port site infections have remarkably enhanced to be a stigma as post laparoscopic morbidity and is still a field of debate and further exploration to surgeons. These port site infections (PSI) are infrequent surgical site infection, complicating the recovery of patients undergoing laparoscopic cholecystectomy. Hence, the necessity of further evaluation of the regarded facts was intended to be explored. Aim: This study was aimed to evaluate the factors that created or provoked PSI after laparoscopic removals of gall-bladder and at analysing which of these factors can be traced and modified to combat PSI in a trail to conquer these complications and to achieve maximum advantages from laparoscopic surgeries. Methods: A prospective descriptive qualitative study conducted on patients who underwent laparoscopic cholecystectomies in our hospital as well as at other facilities remote from our work stations. Factors as gender, site of infected port, types of microorganism, acuteness versus chronicity of the disease, types of infection (superficial or deep infection) and intraoperative spillage of stones, bile or pus were analysed in our study. Swabs were taken for culture and sensitivity tests in all patients who developed infections. Explorations were done under GA for some patients who had deep surgical site infections and wound debridement was done, excisional biopsies were taken for histopathological studies, and tissue samples for Gene-Xpert analysis for detection of Mycobacterium tuberculosis was done. All patients were followed up for six months postoperatively at our surgery unit rooms. Results: Port site infection rate was recorded in 40 cases amongst 340 procedures from the July 2018 to June 2020 (11.76%). A higher rate was observed in female patients 32 cases (9.41%) and 13 cases (3.82%) of acute cholecystitis. Larger number of cases of the PSI were superficial infections (77.5%) with non-specific microorganism in 34 cases (80.0%). Conclusion: We reconciled a significant association of PSI with spillage of bile or stones during the procedure and with acute cholecystitis. Most of the infections are superficial and more common in males. Precautions and protocols should be taken in measure to avoid unnecessary hurry and faulty procedure of sterilization during and prior to the whole surgery to combat PSI effectively. J Shaheed Suhrawardy Med Coll 2020; 12(2): 76-81


INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (11) ◽  
pp. 60-65
Author(s):  
Rajkumar S. Bagali ◽  
Sunil S. Jalalpure ◽  
Sachin D. Shinde ◽  
Ganesh R. Pawar ◽  
Gajanan S. Sanap ◽  
...  

Plant derived herbal formulations and remedies provide an effective means for the treatment of various types of disease that are dogmatic and incurable in other types of systems of medicines, but it is essential to explore and establish the scientific basis for therapeutic action of herbal plant medicines. Bauhinia variegata root ethanolic extract was studied for the hepatoprotective activity against CCl4 induced liver injury in rats. For estimation of hepatoprotective role of B. variegata, total bilirubin count, serum enzymes level and finally histopathological study of liver were performed. This extract’s DPPH radical scavenging potential was also studied. The ethanolic extract of B. variegata root administered orally to animal showed significant depletion in CCL4 induced increased level of SGPT, SGOT, ALP and total bilirubin concentration. Significantly (p<0.05), hepatotoxicity is reversed by treatment with Liv 52 syrup also. For initiation of biochemical analysis, the histopathological studies are provided supportive evidence. This extract also showed better activity in quenching DPPH radical. The ethanolic extract of B. variegata root shows antioxidant property by preventing the formation of trichloromethyl peroxy radicals, and thus reduce tissue damage, which is examined and confirmed by the histopathological studies. Therefore, the hepatoprotective activity of ethanolic extract of B. variegata root may be due to its antioxidant potential. Previously studies have reported that plants containing flavonoids possess antioxidant properties. The antioxidant and hepatoprotective properties of the test plant may be attributed to the presence flavonoids. B. variegata root ethanolic extract is shown to have hepatoprotective and antioxidant action.


Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 47
Author(s):  
Natalia Pachura ◽  
Robert Kupczyński ◽  
Jordan Sycz ◽  
Agata Kuklińska ◽  
Anna Zwyrzykowska-Wodzińska ◽  
...  

Plants of the genus Ilex are widespread throughout the world, with its best-known representative being Ilex paraguraiensis from South America. The European species Ilex aquifolium shows similarities in its terpenoid, sugar and phenolic acid profiles. Using aqueous extracts of Ilex aquifolium as a supplement in Wistar rats showed that, despite the lack of caffeine, it had strong hypocholesterolemic effects. In addition, a reduction in oxidative lipid degradation and a decrease in hepatic steatosis in histopathological studies were observed. The results of this study suggest that extracts from the European species Ilex aquifolium may have potential as an alternative treatment for hyperlipidemia.


2021 ◽  
Vol 11 (4) ◽  
pp. 634-641
Author(s):  
Marissa Divia Dayanti ◽  
I Ketut Berata ◽  
I Ketut Puja

The high population of feral cats in Bali, Indonesia, could be a problem associated with cat welfare and the risk of zoonotic diseases. Gonadectomy or male castration is an option for population control; however, there would be more economical ways to reach this goal. Sterilization using chemicals is an alternative to the surgical method of castration as it is reasonably priced, activated in a short time, and does not affect animal activity after the chemical administration. The present study aimed to evaluate intratesticular injection of Sodium chloride as an agent of chemosterilants in male cats. In the current study, 16 healthy male cats aged nearly one year old were randomly selected. The male cats were allocated into four groups containing four cats in each. Group 1 (control) received bilateral intratesticular injection with 0.25 mL normal saline solution containing 2% Lidocaine. The cats in groups 2, 3, and 4 received bilateral intratesticular injection of 0.25 mL Sodium chloride solution containing 2% Lidocaine with a concentration of 15%, 20%, and 25%, respectively. At the end of the study (after 30 days of injection), the cats were castrated and their testes were removed for sperm quality and histopathological evaluation. The results of the present study indicated that intratesticular injection of Sodium chloride significantly decreased the motility and viability rates, and increased the abnormalities of the sperms. Histopathological studies revealed marked depletion of spermatozoa in the testes and seminiferous tubular degeneration, seminiferous tubular atrophy, and epithelial cell vacuolation. In conclusion, the injection of 20-25% solution of Sodium chloride in testes had the potential as a chemosterilant for chemical sterilization in cats.


2021 ◽  
Vol 2021 ◽  
pp. 1-4
Author(s):  
Ali Mehri ◽  
Aida Ayati afin ◽  
Masoumeh Gharib ◽  
Mohammad Etezadpour

Introduction. Echinococcosis is a zoonotic infection caused by Echinococcus species. Iran is endemic for Echinococcus granulosus. Here, we present a case of hydatidosis in an 85-year-old man, presented with acute, dull, constant, and generalized abdominal pain. A computed tomography scan (CT scan) showed an intact hydatid cyst on the bladder dome and several hydatid cysts in the liver. Open surgery revealed a cyst with hepatic origin, confirmed with histopathological studies. Conclusion. Although a primary abdominal hydatid cyst is very rare, it can be transferred to the abdominal cavity without any rupture as secondary ones. In this case, rupture of the liver wall was the reason for this transfer. As a result, there should be a suspicion of hydatidosis in a patient with a similar presentation.


Author(s):  
Reema B. Gotmare ◽  
R. S. Kushwaha ◽  
Neeraj K. Sharma

Tapentadol Hydrochloride is a Tapentadol is a centrally acting analgesic. It has 33% bioavailability due to its first pass effect and hence possesses problems in the development of oral sustained release formulations. Mucoadhesive thermo reversible in-situ nasal gel of Tapentadol HCl was designed and developed to sustain its release due to the increased nasal residence time of the formulation. Poloxamer 407 (PF 127) was selected as it has excellent thermo sensitive gelling properties. HPMCK4M was added to impart mucoadhesive to the formulation, and PEG 400 was used to enhance the drug release. 32 Factorial designs were employed to assess the effect of concentration of HPMCK4M and PEG 400 on the performance of in-situ nasal gel systematically and to optimize the formulation. An optimized in-situ nasal gel was evaluated for appearance, pH, drug content, gelation temperature, mucoadhesive force, viscosity and ex-vivo permeability of drug through nasal mucosa of a goat. Additionally, this formulation was proved to be safe as histopathological studies revealed no deleterious effect on nasal mucosa of a goat after prolonged exposure of 21 days to the optimized formulation. Thus the release of Tapentadol Hydrochloride can be sustained if formulated in an in-situ nasal gel containing poloxamer 407 to achieve its prolonged action.


Author(s):  
Oyedeji K.O ◽  
Momoh R.O ◽  
Oderinde Gbenga

This study was designed to investigate the effect of penicillin on reproductive function in female Wistar rats. Fifteen female rats (120 – 160 g) were used for the estrous cycle and histopathological studies. Penicillin (17.14 mg/kg) was administered orally on daily basis for 21 and 50 days respectively for the estrous cycle and histological studies. Estrous cycle was carried out using the technique of Marcondes et al., histologies of the ovaries and uteri were also carried out. Data were analysed using descriptive statistics and student’s t-test at p=0.05. Treatment of rats for 21 days with penicillin (17.14 mg/kg) produced significant (p<0.05) increments in the estrous and metestrous phases as well as a significant (p<0.05) reduction in the proestrous phase of the estrous cycle relative to their respective controls. The histopathological study presented with a moderate endometrial congestion. It can therefore be concluded that penicillin probably has a pro-fertility effect with a moderate deleterious effect on the uteri at histological level in female Wistar rats.


Author(s):  
Oyedeji K.O

This study was designed to investigate the effect of artesunate on reproductive function in female Wistar rats. Fifteen female rats (120 – 160 g) were used for the estrous cycle and histopathological studies. Artesunate (1.43 mg/kg) was administered orally on daily basis for 21 and 50 days respectively for the estrous cycle and histological studies. Estrous cycle was carried out using the technique of Marcondes et al., histologies of the ovaries and uteri were also carried out. Data were analysed using descriptive statistics and student’s t-test at p=0.05. Treatment of rats for 21 days with artesunate (1.43 mg/kg) produced significant (p<0.05) increments in the estrous and metestrous phases as well as a significant (p<0.05) reduction in the proestrous phase of the estrous cycle relative to their respective controls. The histopathological study presented with ovarian medullar that is severely congested (hemorrhagic) including expanded lumen of the endometrial glands. It can therefore be concluded that artesunate probably has pro-fertility effect with deleterious effect on the ovaries at histological level in female Wistar rats.


Author(s):  
Oyedeji K.O ◽  
Nwume B. E ◽  
Zachariah Richard

This study was designed to investigate the effect of methotrexate on reproductive function in female Wistar rats. Eighteen female rats (120 – 160 g) were used for the estrous cycle and histopathological studies. Methotrexate (0.071 mg/kg) was administered orally on daily basis for 21 and 50 days respectively for the estrous cycle and histological studies. Estrous cycle was carried out using the technique of Marcondes et al., histologies of the ovaries and uteri were also carried out. Data were analysed using descriptive statistics and student’s t-test at p=0.05. Treatment of rats for 21 days with methotrexate (0.071 mg/kg) produced significant (p<0.05) reductions in the proestrous and estrous phase of the estrous cycle as well as significant (p<0.05) increments in the metestrous and diestrous phases of the estrous cycle relative to their respective controls. The histopathological study revealed that treatment of rats with methotrexate (0.071 mg/kg) for 50 days presented with ovarian medullary part that is congested and oedematous as well as with multiple foci of hemorrhage within the endometria. It can therefore be concluded that that methotrexate probably has dominant pro-fertility effect, but also probably induced deleterious effects on the ovaries and uteri in female Wistar rats.


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