A highly sensitive immuno-polymerase chain reaction assay for Clostridium botulinum neurotoxin type A

Toxicon ◽  
2004 ◽  
Vol 43 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Hai-Yuan Chao ◽  
Yeau-Ching Wang ◽  
Shiao-Shek Tang ◽  
Hwan-Wun Liu
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Clostridium Botulinum ◽  
Botulinum Neurotoxin ◽  
Type A ◽  
Chain Reaction ◽  
Botulinum Neurotoxin Type A ◽  
Highly Sensitive ◽  
Polymerase Chain

Polymerase Chain Reaction for Detection of Type A Clostridium botulinum in Foods

1993 ◽  
Vol 56 (1) ◽  
pp. 18-20 ◽  
Author(s):  
JOSEPH L. FERREIRA ◽  
BARBARA R. BAUMSTARK ◽  
MOSTAFA K. HAMDY ◽  
STEVEN G. MCCAY
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Botulinum ◽  
Brain Heart Infusion ◽  
Type A ◽  
Brain Heart Infusion Broth ◽  
Broth Culture ◽  
Specific Gene ◽  
Gene Probe ◽  
Chain Reaction ◽  
Polymerase Chain

A DNA fragment of the type A Clostridium botulinum neurotoxin gene was demonstrated in canned food products with the polymerase chain reaction (PCR). The fragment, 1340-bp in size, was amplified from green peas, whole kernel corn, green beans, lima beans, black-eyed peas, and turnip greens previously inoculated with type A C. botulinum. The PCR products were identified by agarose gel electrophoresis and also confirmed by dot blot DNA hybridization with a type A specific gene probe. Some inoculated foods were PCR negative but became PCR positive after subculture and overnight incubation in brain heart infusion broth. No PCR amplification products were obtained from uninoculated foods. The procedure was highly sensitive and detected as few as 100 vegetative cells per ml brain heart infusion broth culture.

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