Calcium-dependent disorder-to-order transitions are central to the secretion and folding of the CyaA toxin of Bordetella pertussis, the causative agent of whooping cough

AbstractBordetella pertussis is the causative agent of whooping cough, commonly referred to as pertussis. Although the incidence of pertussis was reduced through vaccination, during the last thirty years it has returned to high levels in a number of countries. This resurgence has been linked to the switch from the use of whole-cell to acellular vaccines. Protection afforded by acellular vaccines appears to be short-lived compared to that afforded by whole cell vaccines. In order to inform future vaccine improvement by identifying immune correlates of protection, a human challenge model of B. pertussis colonisation has been developed. Accurate measurement of colonisation status in this model has required development of a qPCR-based assay to enumerate B. pertussis in samples that distinguishes between viable and dead bacteria. Here we report the development of this assay and its performance in the quantification of B. pertussis from human challenge model samples. This assay has future utility in diagnostic labs and in research where a quantitative measure of both B. pertussis number and viability is required.

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CLINICAL-DIAGNOSTIC VALUE OF BORDETELLA PERTUSSIS GENETIC MARKERS IN CONTACT PERSONS IN FAMILIAL FOCI

Journal Infectology ◽

10.22625/2072-6732-2019-11-1-17-24 ◽

2019 ◽

Vol 11 (1) ◽

pp. 17-24 ◽

Cited By ~ 2

Author(s):

Yu. V. Nesterova ◽

A. Yu. Medkova ◽

I. V. Babachenko ◽

E. G. Semin ◽

E. L. Kalisnikova ◽

...

Keyword(s):

Genetic Markers ◽

Causative Agent ◽

Bordetella Pertussis ◽

Whooping Cough ◽

Bacterial Load ◽

Clinical Signs ◽

Diagnostic Value ◽

Genetic Mutation ◽

Test System ◽

Atypical Form

Goal. Evaluation of duration and frequency of Bordetella pertussis DNA detection in contact persons in family foci of whooping-cough.Materials and methods. 116 persons from 59 family foci of pertussis were examined in contact with sick young children. The DNA of B. pertussis bacteria in nasopharyngeal swabs was detected by real-time PCR (PCR-RV) using a test system developed at Gamaleya Research Institute of Epidemiology and Microbiology (Moscow). The bacterial load and the duration of the release of genomic equivalents (GE) of B. pertussis DNA were determined in dynamics at 1, 3 and 6 months.Results. Among the contact persons in family foci, adults accounted for 59,48%, adolescents and schoolchildren – 10,35% and 12,07% respectively. Cough was absent in 35,34% of contact persons, 20,69% had a rare dry cough, 24,14% had a dry compulsive cough and 19,83% had a typical cough. None of the contact family members were diagnosed with whooping cough, although 64.66% of the patients had clinical signs of the disease, mainly its atypical form (44.83%). Among the carriers of B. pertussis adults accounted for 82.92%, among patients with atypical forms of whooping cough – 51.92%. In the study of nasopharyngeal swabs using the PCR-RV method, it was found that 86.10% of the contact persons detected DNA of B. pertussis. After 3 months in 90% of the contacts, the DNA of pertussis causative agent was detected in a minimum amount of 101-102GE/ml in the sample. After 6 months, B. pertussis was sanitized in 50% of the examined patients. 12.5% of the samples identified avirulent forms of the causative agent of pertussis, formed as a result of movement of IS481 in operon bvgAS.The conclusion. In 86.1% of contact persons in family foci for a long time (from 3 to 6 months), detection of genetic markers of the causative agent of pertussis from the nasopharynx was noted, including 35.34% of those examined in the absence of cough. This, along with the reported genetic mutation in operon bvgAS in 12.5% of cases, can characterize the presence of persistence of B. pertussis, explaining its preservation in circulation in the conditions of mass vaccine prevention.

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Calcium Tightly Regulates Disorder-To-Order Transitions Involved in the Secretion, Folding and Functions of the CyaA Toxin of Bordetella Pertussis, the Causative Agent of Whooping Cough

Biophysical Journal ◽

10.1016/j.bpj.2016.11.2828 ◽

2017 ◽

Vol 112 (3) ◽

pp. 523a ◽

Cited By ~ 1

Author(s):

Darragh P. O’Brien ◽

Sara E. Cannella ◽

Dominique Durand ◽

Véronique Y. Ntsogo Enguéné ◽

Belen Hernandez ◽

...

Keyword(s):

Causative Agent ◽

Bordetella Pertussis ◽

Whooping Cough

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Congenerics

Pertussis ◽

10.1093/oso/9780198811879.003.0011 ◽

2018 ◽

pp. 182-192

Author(s):

Iain MacArthur ◽

Andrew Preston

Keyword(s):

Causative Agent ◽

Bordetella Pertussis ◽

Comparative Studies ◽

Copy Number ◽

Insertion Sequence ◽

Genome Rearrangement ◽

Gene Loss ◽

Whooping Cough ◽

Sequence Element ◽

Insertion Sequence Element

The evolution of Bordetella pertussis from B. bronchiseptica (or a B. bronchiseptica-like ancestor) occurred primarily through gene loss and genome rearrangement, mediated largely through the expansion in the copy number of insertion sequence element repeats in the B. pertussis genome. B. pertussis is attributed as the main causative agent of whooping cough. However, B. parapertussis and B. holmesii also cause disease that is very similar to that caused by B. pertussis (here termed pertussis-like disease). The evolution of B. parapertussis and B. holmesii displays striking similarities to that of B. pertussis and thus this chapter explores what might be gained from comparative studies of B. pertussis, B. parapertussis, and B. holmesii with regard to the understanding of whooping cough.

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Whooping Cough: Bordetella Pertussis is the Causative Agent

Bacterial Diseases ◽

10.2174/9789811473760120010027 ◽

2020 ◽

pp. 108-111

Author(s):

Muhammad Imran Qadir ◽

Ramsha Shahzad

Keyword(s):

Causative Agent ◽

Bordetella Pertussis ◽

Whooping Cough

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Calcium tightly regulates disorder-to-order transitions involved in the secretion, folding and functions of the cyaA toxin of Bordetella pertussis, the causative agent of whooping cough

Toxicon ◽

10.1016/j.toxicon.2017.12.022 ◽

2018 ◽

Vol 149 ◽

pp. 92

Author(s):

Darragh P. O'brien ◽

Sara E. Cannella ◽

Dominique Durand ◽

Véronique Y. Ntsogo Eenguene ◽

Belen Hernandez ◽

...

Keyword(s):

Causative Agent ◽

Bordetella Pertussis ◽

Whooping Cough

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Diphtheria-tetanus-pertussis vaccine combined with Bordetella parapertussis vaccine

Journal of Hygiene ◽

10.1017/s0022172400020398 ◽

1962 ◽

Vol 60 (3) ◽

pp. 289-293 ◽

Cited By ~ 1

Author(s):

Neda Köhler-Kubelka

Keyword(s):

Clinical Examination ◽

Pertussis Vaccine ◽

Bordetella Pertussis ◽

Whooping Cough ◽

Production Test ◽

Post Vaccination ◽

Bordetella Parapertussis ◽

Cross Reactions ◽

Combined Vaccine

Investigations carried out to ascertain the ability of various strains of Bordetella pertussis and B. parapertussis to produce agglutinins have shown that the agglutinin response is considerably greater with B. parapertussis.Children inoculated with a combined vaccine in which the parapertussis element contained B. parapertussis in only one-twelfth of the concentration of B. pertussis in the pertussis element showed agglutinins in their sera in titres well above 1:300 for both organisms. There were no cross-reactions and the serological responses were specific throughout. The vaccine used was the standard diphtheria-tetanus-pertussis (DTP) prophylactic to which had been added a vaccine prepared from recently isolated strains of B. parapertussis.Agglutinin titres of both whooping cough components with the combined vaccine were somewhat lower in mice than was the case when monovalent vaccines were used, but they were considered to be satisfactory.It is suggested that the agglutination production test in mice could be used for the assessment of protective power of B. parapertussis vaccines against infection.I wish to thank Dr Ikić, director of the Institute of Immunology, Zagreb, who enabled me to perform all these examinations, further to Dr B. Mravunac and Dr Z. Radanov for having carried out vaccination in children and for the clinical examination of post vaccination reactions.

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Bordetella pertussis Lipid A Glucosamine Modification Confers Resistance to Cationic Antimicrobial Peptides and Increases Resistance to Outer Membrane Perturbation

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02590-14 ◽

2014 ◽

Vol 58 (8) ◽

pp. 4931-4934 ◽

Cited By ~ 19

Author(s):

Nita R. Shah ◽

Robert E. W. Hancock ◽

Rachel C. Fernandez

Keyword(s):

Immune System ◽

Antimicrobial Peptides ◽

Outer Membrane ◽

Bordetella Pertussis ◽

Lipid A ◽

Whooping Cough ◽

Human Immune System ◽

Cationic Antimicrobial Peptides ◽

Content Type ◽

Membrane Perturbation

ABSTRACTBordetella pertussis, the causative agent of whooping cough, has many strategies for evading the human immune system. Lipopolysaccharide (LPS) is an important Gram-negative bacterial surface structure that activates the immune system via Toll-like receptor 4 and enables susceptibility to cationic antimicrobial peptides (CAMPs). We show modification of the lipid A region of LPS with glucosamine increased resistance to numerous CAMPs, including LL-37. Furthermore, we demonstrate that this glucosamine modification increased resistance to outer membrane perturbation.

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Multiple-Locus Variable-Number Tandem Repeat Analysis of Dutch Bordetella pertussis Strains Reveals Rapid Genetic Changes with Clonal Expansion during the Late 1990s

Journal of Bacteriology ◽

10.1128/jb.186.16.5496-5505.2004 ◽

2004 ◽

Vol 186 (16) ◽

pp. 5496-5505 ◽

Cited By ~ 99

Author(s):

Leo M. Schouls ◽

Han G. J. van der Heide ◽

Luc Vauterin ◽

Paul Vauterin ◽

Frits R. Mooi

Keyword(s):

The Netherlands ◽

Tandem Repeat ◽

Bordetella Pertussis ◽

Minimum Spanning Tree ◽

Whooping Cough ◽

Variable Number Tandem Repeat ◽

Clonal Expansion ◽

Variable Number ◽

Multiple Locus ◽

Repeat Analysis

ABSTRACT Bordetella pertussis, the causative agent of whooping cough, has remained endemic in The Netherlands despite extensive nationwide vaccination since 1953. In the 1990s, several epidemic periods have resulted in many cases of pertussis. We have proposed that strain variation has played a major role in the upsurges of this disease in The Netherlands. Therefore, molecular characterization of strains is important in identifying the causes of pertussis epidemiology. For this reason, we have developed a multiple-locus variable-number tandem repeat analysis (MLVA) typing system for B. pertussis. By combining the MLVA profile with the allelic profile based on multiple-antigen sequence typing, we were able to further differentiate strains. The relationships between the various genotypes were visualized by constructing a minimum spanning tree. MLVA of Dutch strains of B. pertussis revealed that the genotypes of the strains isolated in the prevaccination period were diverse and clearly distinct from the strains isolated in the 1990s. Furthermore, there was a decrease in diversity in the strains from the late 1990s, with a remarkable clonal expansion that coincided with the epidemic periods. Using this genotyping, we have been able to show that B. pertussis is much more dynamic than expected.

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