Ochratoxin A and fumonisin B1 exhibit synergistic cytotoxic effects by inducing apoptosis on rat liver cells

Toxicon ◽  
2020 ◽  
Vol 181 ◽  
pp. 19-27
Author(s):  
Haiyue Wang ◽  
Yujia Wei ◽  
Ying Xie ◽  
Chao Yan ◽  
Hongzhen Du ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rat Liver ◽  
Fumonisin B1 ◽  
Liver Cells ◽  
Cytotoxic Effects ◽  
Rat Liver Cells

Individual and combined cytotoxic effects of aflatoxin B1, zearalenone, deoxynivalenol and fumonisin B1 on BRL 3A rat liver cells

Toxicon ◽  
2015 ◽  
Vol 95 ◽  
pp. 6-12 ◽  
Author(s):  
Lv-Hui Sun ◽  
Ming-yan Lei ◽  
Ni-Ya Zhang ◽  
Xin Gao ◽  
Chong Li ◽  
...  
Keyword(s):  
Rat Liver ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Liver Cells ◽  
Cytotoxic Effects ◽  
Rat Liver Cells

scholarly journals Comparison of the effects of difenacoum and brodifacoum on the ultrastructure of rat liver cells

2016 ◽  
Vol 67 (3) ◽  
pp. 204-209 ◽  
Author(s):  
Nursel Gül ◽  
Nuri Yiğit ◽  
Fulya Saygılı ◽  
Ebru Demirel ◽  
Ceren Geniş
Keyword(s):  
Rat Liver ◽  
Lipid Droplets ◽  
Liver Cells ◽  
Mitochondrial Morphology ◽  
Cytotoxic Effects ◽  
Cytoplasmic Material ◽  
Transmission Electron ◽  
Biochemical Assays ◽  
Rat Liver Cells ◽  
Contrast Exposure

Abstract We used transmission electron microscopy to examine the cytotoxic effects of the second-generation anticoagulant rodenticides difenacoum and brodifacoum on rat liver. A single dose of difenacoum or brodifacoum was administered to rats by gastric gavage and liver samples were taken after 24 h, four days or seven days. In the livers of rats treated with difenacoum for 24 h, hepatocytes typically showed increased numbers of lysosomes, as well as enlargement of both the perinuclear space and the cisternae of the rough endoplasmic reticulum (RER), while sinusoids were irregularly shaped and contained Kupffer cells. Similar irregularities occurred in brodifacoum-treated rats at the same time point, but additionally increased numbers of vacuoles, damaged mitochondrial cristae, and clumping of chromatin were observed in hepatocytes, and hemolysed erythrocytes were noted in the sinusoids. Comparable findings were made in each group of rats after four days. After seven days of difenacoum treatment, hepatocytes suffered loss of cytoplasmic material and mitochondrial shrinkage, while RER cisternae became discontinuous. In contrast, exposure to brodifacoum for seven days caused the formation of numerous vacuoles and lipid droplets, disordered mitochondrial morphology, chromatin clumping and invagination of the nuclear envelope in hepatocytes. Sinusoids in the livers of rodenticide-treated rats contained an accumulation of dense material, lipid droplets, cells with pycnotic nuclei and hemolysed erythrocytes. Overall, our results show that brodifacoum causes more severe effects in liver cells than difenacoum. Thus our microscopic data along with additional biochemical assays point to a severe effect of rodenticide on vertebrates.

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

Heterogeneity of carboxylesterases in rat liver cells

1992 ◽  
Vol 44 (4) ◽  
pp. 827-829 ◽  
Author(s):  
Rolf Gaustad ◽  
Trond Berg ◽  
Frode Fonnum
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells
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