Genotoxicity assessment by micronucleus assay in peripheral blood lymphocytes and buccal epithelial cells of children with chronic kidney disease

2010 ◽  
Vol 196 ◽  
pp. S168 ◽  
Author(s):  
G. Cakmak Demircigil ◽  
B. Aykanat ◽  
K. Fidan ◽  
U.S. Bayrakci ◽  
B. Buyukkaragoz ◽  
...  
2020 ◽  
Vol 99 (8) ◽  
pp. 792-802
Author(s):  
Natal'ya V. Eremina ◽  
Aliy K. Zhanataev ◽  
Andrey D. Durnev

Introduction. A systematic review and analysis of literature on genotoxic examinations of individuals occupationally exposed to formaldehyde vapors (FAV) when working in pathomorphological laboratories of medical institutions has been performed. Formaldehyde is classified by the WHO International Agency for Research on Cancer as a class I carcinogen. Many studies have been published concerning testification of the genotoxic damage of pathomorphological laboratory personnel working with formaldehyde, identification using various biomonitoring cytogenetic methods, in particular, the micronucleus test in peripheral blood lymphocytes and buccal epithelial cells, a chromosomal aberrations test, and the DNA comet assay.Material and methods. Literature was searched until December 2019 using the MedLine / PubMed database of scientific literature (https://www.ncbi.nlm.nih.gov/PubMed). Key search terms included formaldehyde laboratory micronuclei, formaldehyde laboratory chromosomal aberration, or formaldehyde laboratory DNA comet. Full-text articles published in English in journals with assigned DOIs were considered.Results. All studies reported the presence of FAV in the workplace, while in only half of the cases the level of formaldehyde was not higher than the maximum permissible values. The average exposure to formaldehyde over an 8-hour working day was 0.79 ± 0.43 mg/m3. All studies reported the presence of an increased level of the studied cytogenetic biomarkers compared to controls. A total analysis of the data showed more than a 2.5-fold excess in the level of micronuclei in the peripheral blood lymphocytes of laboratory workers compared with the control groups (8.15 ± 2.57 ‰ vs. 3.56 ± 1.15 ‰; p < 0.05), and more than a 5-fold excess in case of the level of micronuclei in buccal epithelial cells (0.83 ± 0.09 ‰ vs. 0.16 ± 0.01 ‰; p < 0.05).Conclusion. Thus, pathomorphological laboratory personnel exposed to FAV is at potential risk to life and health from the long-term impact of genotoxic eff


Mutagenesis ◽  
2011 ◽  
Vol 26 (5) ◽  
pp. 643-650 ◽  
Author(s):  
G. Cakmak Demircigil ◽  
B. Aykanat ◽  
K. Fidan ◽  
K. Gulleroglu ◽  
U. S. Bayrakci ◽  
...  

Author(s):  
Parisa moeinian ◽  
Rasoul Alizadeh ◽  
Mitra Hakim Shooshtari ◽  
Hossein Mozdarani ◽  
Fatemeh Yousefi Laksari ◽  
...  

2019 ◽  
Vol 56 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Mohammad SHOKRZADEH ◽  
Abbas MOHAMMADPOUR ◽  
Mona MODANLOO ◽  
Melika HASSANI ◽  
Nasrin Ghassemi BARGHI ◽  
...  

ABSTRACT BACKGROUND: Gastric cancer is known as the fourth most common cancer. Current treatments for cancer have damaged the sensitive tissues of the healthy body, and in many cases, cancer will be recurrent. Therefore, need for treatments that are more effective is well felt. Researchers have recently shifted their attention towards antipsychotic dopamine antagonists to treat cancer. The anticancer activities of aripiprazole remain unknown. OBJECTIVE: This study aimed to evaluate the efficacy and safety of aripiprazole on gastric cancer and normal cell lines. METHODS: In this regard, the cytotoxicity and genotoxicity of aripiprazole were investigated in MKN45 and NIH3T3 cell lines by methyl tetrazolium assay and on peripheral blood lymphocytes by micronucleus assay. For this purpose, cells were cultured in 96 wells plate. Stock solutions of aripiprazole and cisplatin were prepared. After cell incubation with different concentrations of aripiprazole (1, 10, 25, 50, 100 and 200 μL), methyl tetrazolium solution was added. For micronucleus assay fresh blood was added to RPMI culture medium 1640 supplemented, and different concentrations of aripiprazole (50, 100 and 200 μL) were added. RESULTS: The finding of present study showed that the IC50 of aripiprazole in the cancer cell line (21.36 μg/mL) was lower than that in the normal cell line (54.17 μg/mL). Moreover, the micronucleus assay showed that the frequency of micronuclei of aripiprazole at concentrations below 200 μM was much less than cisplatin. CONCLUSION: Aripiprazole can be a good cytotoxic compound and good candidate for further studies of cancer therapy.


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