DNA damage kinetics (micronucleus and comet assay) in buccal mucosa cells of subjects after metal fixed appliances

Panoramic X-ray is well known to cause DNA damage and induces cellular death. The aim of the present study was to evaluate the cytotoxicity of radiation exposure from panoramic radiography on human buccal mucosa cells by assessing the cell viability using the simple-trypan blue exclusion test. The genotoxicity effect was evaluated by assessing comet assay score. This research included a total of 20 healthy patients who had panoramic radiography for a routine dental examination. Buccal mucosa cells were collected from all participants before X-ray exposure and at 30 min or 24 h after exposure in Groups 1 and 2, respectively, and subjected to a comet assay and trypan blue exclusion test to assess cell viability and DNA damage. Cell viability was calculated as the ratio of live (translucent) to total counted cells. Comet assay output images were analysed using OpenComet software and a visual score by measuring the percentages of tail DNA and summing the visual score, respectively. A statistically significant (p < 0.05) reduce in cell viability was observed at 30 min after exposure, furthermore there is no more reduction after 24 h. Both comet assay measurements showed a significant (p < 0.05) increase in the percentage of tail DNA and visual score at 30 min after exposure, then tend to decrease after 24 h of exposure, although it was not significant (p > 0.05). The results showed that panoramic radiography interfered cell viability and induced DNA damage in buccal mucosa cells within 30 min after exposure, but these effects were ceased after 24 h.

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Comments on the paper by E. M. Minicucci et al. “DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy”

Clinical and Experimental Medicine ◽

10.1007/s10238-008-0018-9 ◽

2008 ◽

Vol 9 (1) ◽

pp. 77-78 ◽

Cited By ~ 2

Author(s):

Armen Nersesyan

Keyword(s):

Dna Damage ◽

Buccal Mucosa ◽

Malignant Tumours ◽

Buccal Mucosa Cells

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DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy

Clinical and Experimental Medicine ◽

10.1007/s10238-008-0161-3 ◽

2008 ◽

Vol 8 (2) ◽

pp. 79-85 ◽

Cited By ~ 24

Author(s):

E. M. Minicucci ◽

D. A. Ribeiro ◽

B. de Camargo ◽

M. C. Costa ◽

L. R. Ribeiro ◽

...

Keyword(s):

Dna Damage ◽

Buccal Mucosa ◽

Malignant Tumours ◽

Buccal Mucosa Cells

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P60b DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumors undergoing chemotherapy

Oral Oncology Supplement ◽

10.1016/s1744-7895(07)70339-5 ◽

2007 ◽

Vol 2 (1) ◽

pp. 150

Author(s):

E. Minicucci ◽

B. Camargo ◽

M. Costa ◽

D. Ribeiro ◽

D. Salvadori

Keyword(s):

Dna Damage ◽

Buccal Mucosa ◽

Malignant Tumors ◽

Buccal Mucosa Cells

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DNA Damage in Buccal Mucosa Cells of Pre-School Children Exposed to High Levels of Urban Air Pollutants

PLoS ONE ◽

10.1371/journal.pone.0096524 ◽

2014 ◽

Vol 9 (5) ◽

pp. e96524 ◽

Cited By ~ 29

Author(s):

Elisabetta Ceretti ◽

Donatella Feretti ◽

Gaia C V. Viola ◽

Ilaria Zerbini ◽

Rosa M. Limina ◽

...

Keyword(s):

Dna Damage ◽

School Children ◽

Buccal Mucosa ◽

Air Pollutants ◽

Urban Air ◽

Buccal Mucosa Cells

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Micronucleus frequency in buccal mucosa cells of patients with neurodegenerative diseases

Scientific Reports ◽

10.1038/s41598-020-78832-y ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Hauke Reimann ◽

Helga Stopper ◽

Thomas Polak ◽

Martin Lauer ◽

Martin J. Herrmann ◽

...

Keyword(s):

Dna Damage ◽

Neurodegenerative Diseases ◽

Buccal Mucosa ◽

Lifestyle Factors ◽

Direct Link ◽

Buccal Cells ◽

Micronucleus Frequency ◽

Binucleated Cells ◽

Buccal Mucosa Cells ◽

Healthy Participants

AbstractNeurodegenerative diseases show an increase in prevalence and incidence, with the most prominent example being Alzheimer’s disease. DNA damage has been suggested to play a role in the pathogenesis, but the exact mechanisms remain elusive. We enrolled 425 participants with and without neurodegenerative diseases and analyzed DNA damage in the form of micronuclei in buccal mucosa samples. In addition, other parameters such as binucleated cells, karyolytic cells, and karyorrhectic cells were quantified. No relevant differences in DNA damage and cytotoxicity markers were observed in patients compared to healthy participants. Furthermore, other parameters such as lifestyle factors and diseases were also investigated. Overall, this study could not identify a direct link between changes in buccal cells and neurogenerative diseases, but highlights the influence of lifestyle factors and diseases on the human buccal cytome.

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