scholarly journals Micronucleus frequency in buccal mucosa cells of patients with neurodegenerative diseases

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hauke Reimann ◽  
Helga Stopper ◽  
Thomas Polak ◽  
Martin Lauer ◽  
Martin J. Herrmann ◽  
...  
Keyword(s):  
Dna Damage ◽  
Neurodegenerative Diseases ◽  
Buccal Mucosa ◽  
Lifestyle Factors ◽  
Direct Link ◽  
Buccal Cells ◽  
Micronucleus Frequency ◽  
Binucleated Cells ◽  
Buccal Mucosa Cells ◽  
Healthy Participants

AbstractNeurodegenerative diseases show an increase in prevalence and incidence, with the most prominent example being Alzheimer’s disease. DNA damage has been suggested to play a role in the pathogenesis, but the exact mechanisms remain elusive. We enrolled 425 participants with and without neurodegenerative diseases and analyzed DNA damage in the form of micronuclei in buccal mucosa samples. In addition, other parameters such as binucleated cells, karyolytic cells, and karyorrhectic cells were quantified. No relevant differences in DNA damage and cytotoxicity markers were observed in patients compared to healthy participants. Furthermore, other parameters such as lifestyle factors and diseases were also investigated. Overall, this study could not identify a direct link between changes in buccal cells and neurogenerative diseases, but highlights the influence of lifestyle factors and diseases on the human buccal cytome.

DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy

2008 ◽  
Vol 8 (2) ◽  
pp. 79-85 ◽  
Author(s):  
E. M. Minicucci ◽  
D. A. Ribeiro ◽  
B. de Camargo ◽  
M. C. Costa ◽  
L. R. Ribeiro ◽  
...  
Keyword(s):  
Dna Damage ◽  
Buccal Mucosa ◽  
Malignant Tumours ◽  
Buccal Mucosa Cells

P60b DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumors undergoing chemotherapy

2007 ◽  
Vol 2 (1) ◽  
pp. 150
Author(s):  
E. Minicucci ◽  
B. Camargo ◽  
M. Costa ◽  
D. Ribeiro ◽  
D. Salvadori
Keyword(s):  
Dna Damage ◽  
Buccal Mucosa ◽  
Malignant Tumors ◽  
Buccal Mucosa Cells

scholarly journals DNA Damage in Buccal Mucosa Cells of Pre-School Children Exposed to High Levels of Urban Air Pollutants

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96524 ◽  
Author(s):  
Elisabetta Ceretti ◽  
Donatella Feretti ◽  
Gaia C V. Viola ◽  
Ilaria Zerbini ◽  
Rosa M. Limina ◽  
...  
Keyword(s):  
Dna Damage ◽  
School Children ◽  
Buccal Mucosa ◽  
Air Pollutants ◽  
Urban Air ◽  
Buccal Mucosa Cells

scholarly journals Micronuclei and Other Nuclear Anomalies in Exfoliated Buccal Mucosa Cells in Breast Cancer

2021 ◽  
Vol 5 (3) ◽  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Genomic Instability ◽  
Buccal Mucosa ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Buccal Cells ◽  
Healthy Women ◽  
Buccal Mucosa Cells ◽  
Cytome Assay

Objectives: This study designed to assess the genomic instability between healthy women and women with breast cancer by means of buccal cells micronucleus (MN) cytome assay. Methods: The current study comprised 25 healthy women and 30 breast cancer patients. The exfoliated cells of buccal mucosa were taken after each participants rinse their mouths with tap water. The micronucleated cell and nuclear anomalies were analyzed under a total magnification of X1000, 2000 cells per subjects ( patient and control group) were scored and the frequencies of nuclear anomalies including MN, binucleates (BN), Pycnotic cell, karyolysis (KL) and karyorrhexis (KR) were evaluated in exfoliated buccal mucosa cells of women with primary BC and healthy women. Results: The frequencies of micronuclei and all nuclear anomalies in buccal cells of BC patients were significantly increased compared with the controls. (For Binucleates cells only, p<0.001; in all other cases, P < 0.0001). The mean scores of micronuclei and all nuclear anomalies for the breast cancer patients were (10.66±0.3845, 6.20±0.26, 8.40±0. 22, 18.40±0. 34, 19.13 ±0.40) were significantly higher than that of healthy women). Conclusion: Elevated frequency of micronucleated cells and all nuclear anomalies in the buccal mucosa of breast cancer patients reveal the genomic instability. These findings propose that the buccal MN-cytome assay can be used to measure both genotoxic and cytotoxic effects in primary cancer patients.

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