Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from Kabylie, Algeria

Background: Tick-borne bacteria, Anaplasma platys and Ehrlichia canis are well recognized as the etiology of anemia and thrombocytopenia in dogs. The clinical signs of anaplasmosis and ehrlichiosis range from asymptomatic to severe symptoms. There are insufficient studies about epidemiological surveys of these blood parasites, also the association of infections with the hematological study. Aim: This study aimed to screen A. platys and E. canis in naturally infected dogs and the effects of the infection on the levels of packed cell volume (PCV) and platelet count. Materials and Methods: A total of 68 blood samples were collected from free-roaming dogs at Nong Kung Sri district, Kalasin Province, Thailand, and examined for A. platys and E. canis infection by polymerase chain reaction (PCR) and measured PCV levels and platelet count. Results: Using nested PCR, 42.65% of dogs were infected with one or two pathogens. The molecular detection of anaplasmosis and ehrlichiosis in this population was 29.4% (95% confidence interval [CI]: 18.98-41.71) and 25% (95% CI: 14.4-35.3), respectively. Coinfection occurred at 11.8% (95% CI: 5.22-21.87). Infection with E. canis and coinfection showed significant association with PCV levels (p<0.05) while A. platys infection showed no statistical relationship. Infection with A. platys, E. canis, and coinfection had a non-significant correlation with platelet count (p>0.05). Conclusion: This study provides data of anaplasmosis and ehrlichiosis in free-roaming dogs which indicated that these zoonotic diseases are widespread and require for disease frequency determination, especially in Kalasin Province of Thailand where data of tick-borne infections in dogs have not been reported.

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Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from the North of Portugal

The Veterinary Journal ◽

10.1016/j.tvjl.2008.10.009 ◽

2010 ◽

Vol 183 (2) ◽

pp. 232-233 ◽

Cited By ~ 26

Author(s):

Luís Cardoso ◽

Joana Tuna ◽

Lisete Vieira ◽

Yael Yisaschar-Mekuzas ◽

Gad Baneth

Keyword(s):

Molecular Detection ◽

Ehrlichia Canis ◽

Anaplasma Platys ◽

The North

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Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys and Ehrlichia canis in dogs on Costa Rica

Acta Parasitologica ◽

10.1515/ap-2015-0003 ◽

2014 ◽

Vol 60 (1) ◽

Cited By ~ 2

Author(s):

Lanjing Wei ◽

Patrick Kelly ◽

Kate Ackerson ◽

Heba S. El-Mahallawy ◽

Bernhard Kaltenboeck ◽

...

Keyword(s):

Costa Rica ◽

Dirofilaria Immitis ◽

Molecular Detection ◽

Ehrlichia Canis ◽

Hepatozoon Canis ◽

Anaplasma Platys ◽

Vector Borne Diseases ◽

Tropical Areas ◽

Vector Borne ◽

Babesia Spp

AbstractAlthough vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas, there is little information on these conditions in Costa Rica. In PCRs of blood from dogs in Costa Rica, we did not detect DNAs of Rickettsia (R.) felis and Coxiella (C.) burnetii but we did find evidence of infection with Dirofilaria (D.) immitis (9/40, 22.5%), Hepatozoon (H.) canis (15/40, 37.5%), Babesia spp. (10/40, 25%; 2 with B. gibsoni and 8 with B. vogeli), Anaplasma (A.) platys (3/40, 7.5%) and Ehrlichia (E.) canis (20/40, 50%). Nine dogs (22.5%) were free of any vector-borne pathogens while 14 (35%) were infected with a single pathogen, 11 (27.5%) with two, 4 (10%) with three, 1 (2.5%) with four, and 1 (2.5%) with five pathogens. Dogs in Costa Rica are commonly infected with vector-borne agents

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