Modulation of the humoral and cellular immune response in Aβ immunotherapy by the adjuvants monophosphoryl lipid A (MPL), cholera toxin B subunit (CTB) and E. coli enterotoxin LT(R192G)

Vaccine ◽  
2005 ◽  
Vol 23 (44) ◽  
pp. 5149-5159 ◽  
Author(s):  
Marcel Maier ◽  
Timothy J. Seabrook ◽  
Cynthia A. Lemere
Keyword(s):  
Immune Response ◽  
Lipid A ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
E Coli ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Monophosphoryl Lipid A ◽  
Monophosphoryl Lipid ◽  
Cellular Immune

Circulating cellular immune response to oral immunization of humans with cholera toxin B-subunit

Vaccine ◽  
1993 ◽  
Vol 11 (2) ◽  
pp. 119-121 ◽  
Author(s):  
David J.M. Lewis ◽  
Luiz R.R. Castello-Branco ◽  
Pavel Novotny ◽  
Gordon Dougan ◽  
Terence A. Poulton ◽  
...  
Keyword(s):  
Immune Response ◽  
Cholera Toxin ◽  
Cellular Immune Response ◽  
Oral Immunization ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Cellular Immune

scholarly journals The B subunits of cholera and Escherichia coli heat-labile toxins enhance the immune responses in mice orally immunised with a recombinant live P-fimbrial vaccine for avian pathogenic E. coli

2014 ◽  
Vol 62 (3) ◽  
pp. 293-303
Author(s):  
In-Gyeong Oh ◽  
Chetan Jawale ◽  
John Lee
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Cholera Toxin B Subunit ◽  
Adjuvant Effect ◽  
Toxin B ◽  
E Coli ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Heat Labile ◽  
Enterotoxin B

This study aimed to investigate the adjuvant effect of recombinant attenuatedSalmonellaexpressing cholera toxin B subunit (CTB) andEscherichia coliheat-labile enterotoxin B subunit (LTB) for the P-fimbriae subunit-based vaccine of avian pathogenicE. coli(APEC) in a murine model. The PapA-specific sIgA and IgG responses were significantly enhanced after immunisation with theSalmonella-PapA vaccine in the presence of CTB or LTB. The group immunised with theSalmonella-LTB strain promoted Th1-type immunity, whereas that immunised with theSalmonella-CTB strain produced Th2-type immunity. We concluded that bothSalmonella-CTB and -LTB strains can enhance the immune response to PapA, and that the LTB strain may be a more effective adjuvant for APEC vaccination, which requires higher Th1-type immunity for protection. Thus, our findings provide evidence that immunisation with an adjuvant, LTB, is one of the strategies of developing effective vaccines against P-fimbriated APEC.

scholarly journals Detection of toxigenic Vibrio cholerae by PCR

2011 ◽  
Vol 5 (1) ◽  
pp. 18-21
Author(s):  
Majeed Arsheed Sabbah ◽  
Bilal Kamil Sulaiman ◽  
Kifah, A. Jasim ◽  
Mohammod M. Farhan
Keyword(s):  
Cholera Toxin B Subunit ◽  
Specific Primers ◽  
Toxin B ◽  
E Coli ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Conventional Culture ◽  
Major Virulence Factor ◽  
Ctxb Gene ◽  
Direct Use

holera toxin (CT) is a major virulence factor of V. cholerae causing water diarrhea. The detection of CT-producing V. cholerae using conventional culture-, biochemical- and immunological-based assays is time-consuming, laborious, and requiring more than three days perform. In this work a specific primers for ctxB gene were used for detection of V. cholera in water samples. Few colonies of V. cholera were suspended in water and used as a template in PCR reaction for the detection of ctxB gene. The 391-bp sequence of a gene that codes for the cholera toxin B subunit was amplified by PCR. Direct use of V. cholerae pure culture for PCR replaces the need for DNA extraction or boiling. Increase the concentration of MgCl2 enhances the efficiency of amplification. The specificity of the assay was determined to be specific for V. cholerae but not for, vibrio related bacteria, E. coli, Non-Agglutinable (NAG) V. cholerae, and Aeromonas sp.

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